Sinensetin, eupatorin, 3′-hydroxy-5, 6, 7, 4′-tetramethoxyflavone and rosmarinic acid contents and antioxidative effect of Orthosiphon stamineus from Malaysia

Akowuah, Gabriel Akyirem; Ismail, Zhari; Norhayati, I.; Sadikun, Amirin; Khamsah, S.M

doi:10.1016/j.foodchem.2004.01.008

Cited by 133 publications

(137 citation statements)

References 25 publications

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“…Ginger has been reported to exhibit antibacterial (M. N. J. Gulve & N. D. G. Gulve, 2010;Mohamed et al, 2016), antioxidant (Bellik, 2014), antibiofilm (Kim et al, 2013), anti-gastrointestinal cancer (Prasad & Tyagi, 2015) and antifungal properties (Ficker et al, 2003;Atai et al, 2009). As for O. stamineus, studies have reported on the antioxidant, anti-inflammatory (Akowuah et al, 2004;Yam et al, 2008), antifungal (Hossain et al, 2006) and antibacterial properties of this plant (Ho et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

In vitro Antibacterial Activity of Zingiber officinale and Orthosiphon stamineus on Enterococcus faecalis

Abidin¹,

Abdul-Wahab²,

Ghazi-Ahmad³

et al. 2017

JAS

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This study evaluates the antibacterial effects of Zingiber officinale essential oil and Orthosiphon stamineus water extract against Enterococcus faecalis. The herbs were prepared in various concentrations to determine their minimum inhibitory concentrations (MIC) and growth inhibitory effect. Anti-adhesion activities of the herbs were determined by co-incubation with E. faecalis cultures for 6 and 24 h. Biofilm disruption activities were determined by adding the studied herbs into preformed E. faecalis biofilm. The effects on the morphology of E. faecalis grown as biofilm were studied using scanning electron microscopy (SEM). The MICs of ginger oil and O. stamineus extract were 0.31 and 25 mg/mL, respectively. Between the tested herbs, ginger exhibited greater inhibitory effects on the growth of E. faecalis grown in suspension mode. Both herbs generally showed anti-adhesion activities in inverse concentration-dependent manner. No significant biofilm disruption activities by both herbs were observed. SEM analyses showed E. faecalis cell surface changes in the treated biofilm. The studied herbs may have compromised the integrity of the bacterial cell membrane. These findings suggest that the studied herbs may have better antibacterial activities against E. faecalis in suspension mode compared to biofilm mode, with ginger oil showed greater antibacterial activity compared to O. stamineus extract.

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Section: Discussionmentioning

confidence: 99%

In vitro Antibacterial Activity of Zingiber officinale and Orthosiphon stamineus on Enterococcus faecalis

Abidin¹,

Abdul-Wahab²,

Ghazi-Ahmad³

et al. 2017

JAS

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“…Surprisingly, the FRSA for 100% methanol extract was slightly affected and not significantly different at 4 and 8 hours extraction. Based on previous studies, they were also indicated that sustained time extraction would enhance the amount of oxidation in extracting phenolic compound extract [6]. …”

Section: Free Radical Scavenging Activity (Frsa)mentioning

confidence: 98%

“…The core components of O.stamineus plant are the pharmacologically active polyphenols (the polymethoxylated flavonoids and caffeic acid derivatives) [1]. These elements have been reported to be actual in reducing oxidative stress by inhibiting the formation of lipid peroxidation products in biological systems [2].…”

Section: Introductionmentioning

confidence: 99%

Effect of Differences Methanol Concentration and Extraction Time on the Antioxidant Capacity, Phenolics Content and Bioactive Constituents of Orthosiphon Stamineus Extracts

et al. 2016

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Bioactive compounds of Orthosiphon stamineus have been known to have the beneficial effects on health. Efficient extraction of these beneficial compounds is very important. The study was conducted to determine the efficiency of methanol as an extraction solvent for bioactive compound extraction of O. stamineus raw materials. Leaf sample was extracted in 25, 50 and 100% methanol and distilled water for 2, 4 and 8 hours at 40 0 C. The free radical scavenging activity (FRSA) method was used to determine antioxidant capacity and Follin-Ciocalteu method was used to determine samples total phenolic compounds. The quantification of bioactive constituents by using the high performance liquid chromatography (HPLC) and the standard markers was used for this analysis such as rosmarinic acid (RA), sinensetin (SEN) and 3-hydroxy-5,6,7,4-tetramethoxyflavone (TMF). The current research shows that the yield of RA concentration was high in 50% methanol extracts at 2, 4 and 8 h of extraction. For the FRSA result showed that methanol at 50% and 100% was the most effective concentration levels inhibition between 45-55% at 8 hours' time extraction. For the total phenolic compound, the 50% methanol concentration in O. stamineus leaf had the highest value compared with 0%, 25% and 100% methanol concentration. But, it was observed that time extraction did not influence the TPC values for different concentrations of methanolic extracts. These indicated that time extraction had affected the total capacities of antioxidant and 50% methanolic extracts was the best concentration for indicated the TPC in the O. Storminess leaf.

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“…The TFC of O. stamineus extract was slightly higher than P. niruri extract with 21.6 ± 0.03 and 14.8 ± 0.07 mg QE/g dry extract, respectively (Table 3). O. stamineus contains several chemically active constituents, but one of the most important classes of compounds is the phenolic group [70]. A study of 50% ethanol extract of O. stamineus leaves for its anti-tumor potential showed an ability to inhibit colon tumor in mice could be attributed mainly to its antioxidant-rich polyphenolic contents, the caffeic acid derivatives, polymethoxylated flavonoids and terpenes; particularly rosmarinic acid, eupatorin, sinensetin, 3′-hydroxy-5,6,7,4′-tetramethoxyflavone, and betulinic acid [71].…”

Section: Tpc and Tfc Of O Stamineus And P Niruri Extractsmentioning

confidence: 99%

Anti-obesity Potential of Selected Tropical Plants via Pancreatic Lipase Inhibition

Alias¹,

Leow²,

Ali³

et al. 2017

AOWMC

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Natural products are a vast source of potential compounds that can be developed as anti-obesity agent. One of the mechanisms of anti-obesity agents is inhibition of pancreatic lipase. Assay of 24 crude extracts for their in vitro activity against porcine pancreatic lipase (PPL) detected four extracts demonstrating high (>70%) inhibition, seven extracts had medium (30-70%) inhibition and the remaining 13 extracts exhibited low (<30%) inhibition when incubated with PPL at a concentration of 500 µg/ml for 10 min at 37°C. Phyllanthus niruri extract displayed the most potent PPL inhibitor, followed by Orthosiphon stamineus, Murraya paniculata and Averrhoa bilimbi with the IC50 value of 27.7<34.7< 41.5<55.2 µg/ml, respectively. P. niruri & O. stamineus (the best two extracts) showed noncompetitive and uncompetitive inhibition, respectively. P. niruri & O. stamineus displayed total phenolic content of 431.0 ± 0.01 and 103.0 ± 0.01 mg GAE/g dry extract, while total flavonoid content of 14.8 ± 0.07 and 21.6 ± 0.03 mg QE/g dry extract, respectively. Both P. niruri & O. stamineus extracts showed high antioxidant activity, with EC50 values of 8.4 and 26.3 µg/ml, respectively. The results suggest that P. niruri & O. stamineus may be beneficial for obesity treatment via pancreatic lipase inhibition action.

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Sinensetin, eupatorin, 3′-hydroxy-5, 6, 7, 4′-tetramethoxyflavone and rosmarinic acid contents and antioxidative effect of Orthosiphon stamineus from Malaysia

Cited by 133 publications

References 25 publications

In vitro Antibacterial Activity of Zingiber officinale and Orthosiphon stamineus on Enterococcus faecalis

In vitro Antibacterial Activity of Zingiber officinale and Orthosiphon stamineus on Enterococcus faecalis

Effect of Differences Methanol Concentration and Extraction Time on the Antioxidant Capacity, Phenolics Content and Bioactive Constituents of Orthosiphon Stamineus Extracts

Anti-obesity Potential of Selected Tropical Plants via Pancreatic Lipase Inhibition

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