Single administration of di(n-butyl) phthalate delays spermatogenesis in prepubertal rats

Alam, Mohammad Shah; Andrina, Bibin Bintang; Tay, Tat Wei; Tsunekawa, Naoki; Kanai‐Azuma, Masami; Kurohmaru, Masamichi

doi:10.1016/j.tice.2010.02.004

Cited by 21 publications

(15 citation statements)

References 31 publications

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“…However, the majority of published studies exclusively focused on EDCs exposure during gestation, as conditions associated with testicular dysgenesis syndrome are considered to initiate from aberrancies during fetal development. Other studies revealed that prepubertal rats exposed to phthalates at 0.4–2.2 g/kg/day exhibited altered seminiferous epithelium and delayed spermatogenesis ( 41 , 42 ); however no changes in AGD were reported in the previous reports, even with the highest dose of DEHP and its combination with GEN. In the current study, there was no significant alternation in AGD and adjusted AGD among the different treatment groups; a potential explanation is that the effects of DEHP, single exposure or in combination with GEN, were age-specific in this species.…”

Section: Discussionmentioning

confidence: 80%

Genistein attenuates di‑(2‑ethylhexyl) phthalate-induced testicular injuries via activation of Nrf2/HO‑1 following prepubertal exposure

Zhang

Gao

et al. 2018

Int J Mol Med

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Di-(2-ethylhexyl) phthalate (DEHP) and genistein (GEN) are of the most common endocrine disrupting chemicals (EDCs) present in the environment or the diet. However, investigation of the effects of acute exposure to these two EDCs during prepuberty has been lacking. In this study, DEHP and GEN were administrated to prepubertal male Sprague-Dawley rats by gavage from PND22 to PND35 with vehicle control, GEN 50 mg/kg body weight (bw)/day, DEHP50, 150 and 450 mg/kg bw/day, and combined treatment. Reproductive parameters including testis weight, anogenital distance and organ coefficient were evaluated on PND36. Enzyme activity involved in the regulation of testicular redox state as well as expression of genes and proteins related to anti-oxidative ability and apoptosis were also investigated. The results revealed that by PND36, DEHP treatment had significantly decreased the testis weight, organ coefficient, testicular anti-oxidative enzyme activities and caused tubular vacuolation; however, co-administration of GEN partially alleviated DEHP-induced testicular injuries and enhanced testicular anti-oxidative enzyme activities and upregulated the expression of NF-E2 related factor 2 and heme oxygenase-1, which indicated that GEN partially attenuated DEHP-induced male reproductive system damage through anti-oxidative action following acute prepubertal exposure to DEHP. Thus, GEN may have use in attenuating the damaging effects of other EDCs that lead to reproductive disorders.

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Section: Discussionmentioning

confidence: 80%

Genistein attenuates di‑(2‑ethylhexyl) phthalate-induced testicular injuries via activation of Nrf2/HO‑1 following prepubertal exposure

Zhang

Gao

et al. 2018

Int J Mol Med

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“…In one study of six girls, no effect of phthalate exposure during infancy on puberty was reported (Rais-Bahrami et al, 2004). However, as exposure was remote to the time of puberty assessment and animal studies have shown that the effects of phthalates are reversible (Alam et al, 2010; Boekelheide et al, 2009; Oishi 1985; Saffarini et al, 2012), any potential effect of phthalate exposure may have been missed. Other studies of puberty in girls yielded conflicting results.…”

Section: Epidemiological Literaturementioning

confidence: 99%

Reproductive and developmental effects of phthalate diesters in females

Kay

Chambers

Foster

2013

Critical Reviews in Toxicology

281

176

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Phthalate diesters, widely used in flexible plastics and consumer products, have become prevalent contaminants in the environment. Human exposure is ubiquitous and higher phthalate metabolite concentrations documented in patients using medications with phthalate-containing slow release capsules raises concerns for potential health effects. Furthermore, animal studies suggest that phthalate exposure can modulate circulating hormone concentrations and thus may be able to adversely affect reproductive physiology and the development of estrogen sensitive target tissues. Therefore, we conducted a systematic review of the epidemiological and experimental animal literature examining the relationship between phthalate exposure and adverse female reproductive health outcomes. The epidemiological literature is sparse for most outcomes studied and plagued by small sample size, methodological weaknesses, and thus fails to support a conclusion of an adverse effect of phthalate exposure. Despite a paucity of experimental animal studies for several phthalates, we conclude that there is sufficient evidence to suggest that phthalates are reproductive toxicants. However, we note that the concentrations needed to induce adverse health effects are high compared to the concentrations measured in contemporary human biomonitoring studies. We propose that the current patchwork of studies, potential for additive effects and evidence of adverse effects of phthalate exposure in subsequent generations and at lower concentrations than in the parental generation support the need for further study.

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“…Vacuolations (appearance of empty spaces in the seminiferous tubules), that appear in our testis section are smiler to vacuolation singe that found in the studies of Ahmed et al (25) and where evaluate the effect of nanoparticles on testes of adult albino rats and they indicate of presence intracellular vacuolations in Sertoli and Leydig cells which are a spermatogenic cells., Vacuolation are considered as the first morphological sign of testicular injury. The presence of vacuoles in the cytoplasm of the Sertoli cell denoted direct damage to this cell and reflected its early response to injury as indicated by Alam et al (26) , Nashwa et al (27) . This vacuolation was explained as a result of the autophagosomes created during the phagocytosis of necrotic germ cells by Sertoli cells.…”

Section: Discussionmentioning

confidence: 89%

Histological Effect of Green Synthesized Silver Nanoparticles Agnps on Albino Rat Testis

Jasem

Abas²

2023

The Egyptian Journal of Hospital Medicine

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Background: According to the data, AgNPs are being utilized more frequently for industrial, consumer, medical, food, and health care reasons as well as for other purposes due to their unique physical and chemical characteristics. Despite NPs' benefits, a variety of issues have increased the risk of toxicity in both people and animals. Objective: main objective of this study is to evaluate the effects of AgNPs produced using a green technique from plant extract (Borago officinalis) on the reproductive system (testis). Methods: Borago officinalis flower extracts are used as reducing agents and stabilizing agents in the production of silver nanoparticles, which is an environmentally beneficial process. 40 adult male rats are separated into 4 equally sized groups. The groups were given distilled water treatment, oral plant extract (5 mg/kg), and intraperitoneal injections of AgNPs 30, 50 mg/kg After 35 days, we evaluated the changes in the tissue of the testis. How were the four groups divided? Results: The results showed that AgNPs significantly increased MDA levels in animal groups treated with various AgNP concentrations as compared to control groups treated only with Borage extract. Additionally, Testosterone levels in animals treated with Borage extract decreased non-significantly as compared to the control group, which experienced a significant decline in testosterone when treated with various AgNP concentrations. Conclusion: despite being made from plant extract, AgNPs have a detrimental effect on the male reproductive system by changing the testicular functions, particularly the production of testosterone, in contrast to the benign nature of the crude Borage flower extract. Keywords silver nanoparticles (AgNPs), green synthesis, tissue testis, TEM.

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Single administration of di(n-butyl) phthalate delays spermatogenesis in prepubertal rats

Cited by 21 publications

References 31 publications

Genistein attenuates di‑(2‑ethylhexyl) phthalate-induced testicular injuries via activation of Nrf2/HO‑1 following prepubertal exposure

Genistein attenuates di‑(2‑ethylhexyl) phthalate-induced testicular injuries via activation of Nrf2/HO‑1 following prepubertal exposure

Reproductive and developmental effects of phthalate diesters in females

Histological Effect of Green Synthesized Silver Nanoparticles Agnps on Albino Rat Testis

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