Single- and mixed-species biofilm formation by Escherichia coli O157:H7 and Salmonella, and their sensitivity to levulinic acid plus sodium dodecyl sulfate

Chen, Dong; Zhao, Tong; Doyle, Michael P.

doi:10.1016/j.foodcont.2015.04.006

Cited by 56 publications

(27 citation statements)

References 41 publications

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“…Formation of biofilms by DEC was evaluated using a method described by Chen et al with slight modifications [25]. About 200 µL E. coli broth culture was prepared with glucose.…”

Section: Biofilm Formationmentioning

confidence: 99%

The Frequency of Fluoroquinolone Resistant Enterotoxigenic Escherichia coli from Stools of Diarrheic Children in Ebonyi State, Nigeria

David

Yameen

Igwenyi

et al. 2019

Preprint

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Enterotoxigenic Escherichia coli (ETEC) is an important cause of acute childhood diarrhea. The evaluation of ETEC in children is important for therapeutic and economic purposes. Hence, this study aimed to evaluate the frequency of ETEC among diarrheic children, their multidrug and fluoroquinolone resistant pattern. A total of twenty diarrheagenic E. coli (DEC) isolates were gotten from hundred diarrheal samples using biochemical and molecular methods. Multiplex PCR was used to detect the presence of four different pathological types of DEC. Disk diffusion method was used to determine the antibiotic susceptibility of the organisms. Biofilm formation was detected by thiazoylblue tetrazolium bromide dye in a 96-well plate. Results showed that ETEC represented 30% of the DEC, of which 80% were multidrug and fluoroquinolone resistant. The biofilm production abilities of all the ETEC were found to exist within weak, moderate and strong biofilm producers. We observed a high ETEC frequency and rapid emergence of multidrug/fluoroquinolone resistance, suggesting that it is one of the most important causes of frequent drug resistant diarrhea in children in this region.

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“…Formation of biofilms by DEC was evaluated using a method described by Chen et al with slight modifications [25]. About 200 µL E. coli broth culture was prepared with glucose.…”

Section: Biofilm Formationmentioning

confidence: 99%

The Frequency of Fluoroquinolone Resistant Enterotoxigenic Escherichia coli from Stools of Diarrheic Children in Ebonyi State, Nigeria

David

Yameen

Igwenyi

et al. 2019

Preprint

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“…A food-grade and non-chlorine-based microbicide, containing just two chemicals, levulinic acid and sodium dodecyl sulfate, SDS was developed and thoroughly evaluated in our lab and other labs for effectively killing foodborne pathogens in poultry [12], meat [13], produce [12], and seeds; especially for removal of biofilms in processing plants [6]. This microbicide can also remove dental biofilm in vitro and is 10-fold better than Listerine and when applied in animal mucus for either short term or long term there were no pathological change when compared with water only [9].…”

Section: Stecmentioning

confidence: 99%

“…Epidemiological investigation and animal model studies have revealed that cattle are major reservoir for E. coli O157:H7 and other STEC [3][4][5][6][7][8]10,11]. Recent data indicated that isolation rate of E. coli O157:H7 in individual cattle were ranged from 5 to 20%, with enumeration rate in feces at <100 to >10 4 CFU/g [3,8].…”

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confidence: 99%

Approaches for Reduction of Shiga Toxin-Producing Escherichia coli and Salmonella on Hide of Cattle

Zhao¹

2016

J Food Microbiol Saf Hyg

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“…Enteritidis within a pH range of 5.0–7.2 compared with a control group (pH 7.2); however, no difference was observed in planktonic cell numbers. Several studies have focused on the resistance of biofilm formation to acid stress at different time points, such as short-term or long-term acid stress (Kubota et al, 2008 ; Chen et al, 2015 ; Wang et al, 2016 ). However, the molecule mechanisms underlying the gene expression networks that function during biofilm formation remain unclear, in particular the small non-coding RNAs (sRNAs).…”

Section: Introductionmentioning

confidence: 99%

Preliminary Transcriptome Analysis of Mature Biofilm and Planktonic Cells of Salmonella Enteritidis Exposure to Acid Stress

et al. 2017

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Salmonella has emerged as a well-recognized food-borne pathogen, with many strains able to form biofilms and thus cause cross-contamination in food processing environments where acid-based disinfectants are widely encountered. In the present study, RNA sequencing was employed to establish complete transcriptome profiles of Salmonella Enteritidis in the forms of planktonic and biofilm-associated cells cultured in Tryptic Soytone Broth (TSB) and acidic TSB (aTSB). The gene expression patterns of S. Enteritidis significantly differed between biofilm-associated and planktonic cells cultivated under the same conditions. The assembled transcriptome of S. Enteritidis in this study contained 5,442 assembled transcripts, including 3,877 differentially expressed genes (DEGs) identified in biofilm and planktonic cells. These DEGs were enriched in terms such as regulation of biological process, metabolic process, macromolecular complex, binding and transferase activity, which may play crucial roles in the biofilm formation of S. Enteritidis cultivated in aTSB. Three significant pathways were observed to be enriched under acidic conditions: bacterial chemotaxis, porphyrin-chlorophyll metabolism and sulfur metabolism. In addition, 15 differentially expressed novel non-coding small RNAs (sRNAs) were identified, and only one was found to be up-regulated in mature biofilms. This preliminary study of the S. Enteritidis transcriptome serves as a basis for future investigations examining the complex network systems that regulate Salmonella biofilm in acidic environments, which provide information on biofilm formation and acid stress interaction that may facilitate the development of novel disinfection procedures in the food processing industry.

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Single- and mixed-species biofilm formation by Escherichia coli O157:H7 and Salmonella, and their sensitivity to levulinic acid plus sodium dodecyl sulfate

Cited by 56 publications

References 41 publications

The Frequency of Fluoroquinolone Resistant Enterotoxigenic Escherichia coli from Stools of Diarrheic Children in Ebonyi State, Nigeria

The Frequency of Fluoroquinolone Resistant Enterotoxigenic Escherichia coli from Stools of Diarrheic Children in Ebonyi State, Nigeria

Approaches for Reduction of Shiga Toxin-Producing Escherichia coli and Salmonella on Hide of Cattle

Preliminary Transcriptome Analysis of Mature Biofilm and Planktonic Cells of Salmonella Enteritidis Exposure to Acid Stress

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