Single and mixture effects of aquatic micropollutants studied in precision-cut liver slices of Atlantic cod (Gadus morhua)

Bizarro, Cristina; Eide, Marta; Hitchcock, Daniel J.; Goksøyr, Anders; Ortiz-Zarragoitia, Maren

doi:10.1016/j.aquatox.2016.06.013

Cited by 14 publications

(7 citation statements)

References 64 publications

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“…In mice, basal levels of Cyp3a11 expression was elevated approximately 4-fold in the livers of PXR knockouts relative to their wild-type counterparts 54 , suggesting that PXR also has a repressing transcriptional role. We recently found a significant decrease in cod cyp3a expression in PCLS following a 24-hour exposure to 10 μM ethynylestradiol 55 . This is in line with previous studies that indicate that xenoestrogens affect the expression and activity of Cyp3a and Cyp1a in Atlantic cod and Atlantic salmon ( Salmo salar ) 8 , 56 , 57 , and suggests that the estrogen receptor (Er) signaling pathway also is involved in controlling cyp3a expression in these species.…”

Section: Discussionmentioning

confidence: 95%

Independent losses of a xenobiotic receptor across teleost evolution

Eide

Rydbeck

Tørresen

et al. 2018

Sci Rep

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Sensitivity to environmental stressors largely depend on the genetic complement of the organism. Recent sequencing and assembly of teleost fish genomes enable us to trace the evolution of defense genes in the largest and most diverse group of vertebrates. Through genomic searches and in-depth analysis of gene loci in 76 teleost genomes, we show here that the xenosensor pregnane X receptor (Pxr, Nr1i2) is absent in more than half of these species. Notably, out of the 27 genome assemblies that belong to the Gadiformes order, the pxr gene was only retained in the Merluccidae family (hakes) and Pelagic cod (Melanonus zugmayeri). As an important receptor for a wide range of drugs and environmental pollutants, vertebrate PXR regulate the transcription of a number of genes involved in the biotransformation of xenobiotics, including cytochrome P450 enzymes (CYP). In the absence of Pxr, we suggest that the aryl hydrocarbon receptor (Ahr) have evolved an extended regulatory role by governing the expression of certain Pxr target genes, such as cyp3a, in Atlantic cod (Gadus morhua). However, as several independent losses of pxr have occurred during teleost evolution, other lineages and species may have adapted alternative compensating mechanisms for controlling crucial cellular defense mechanisms.

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Section: Discussionmentioning

confidence: 95%

Independent losses of a xenobiotic receptor across teleost evolution

Eide

Rydbeck

Tørresen

et al. 2018

Sci Rep

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“…Increased research efforts are directed towards Atlantic cod ( Gadus morhua ) and its role as a key species in environmental investigations focusing on ecosystem sustainability and human food safety [ 1 , 2 , 3 , 4 , 5 ]. One focus area is the environmental risks stemming from the constant flux of oil-related polycyclic aromatic hydrocarbons (PAHs) into the habitats and spawning grounds of Atlantic cod [ 2 , 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…It is therefore of high significance to ensure continuous evaluation of the extent of PAH contamination in the marine environment. Presently, large efforts are being made to decode the Atlantic cod toxicology systems to find novel tools applicable for environmental monitoring and risk assessment [ 3 , 4 , 5 ]. A sensitive proteomic marker for PAH exposure is the detection of PAHs covalently bound to albumin (i.e.…”

Section: Introductionmentioning

confidence: 99%

Identification of an albumin-like protein in plasma of Atlantic cod ( Gadus morhua ) and its biomarker potential for PAH contamination

Enerstvedt

Sydnes

Pampanin

2017

Heliyon

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Increased research efforts are currently focusing on Atlantic cod (Gadus morhua) and its significance for monitoring the contaminant situation in marine environments. Polycyclic aromatic hydrocarbons (PAHs) are well known toxic and carcinogenic compounds, thus continuous monitoring is required to ensure ecosystem sustainability and human food safety. A sensitive biomarker of PAH exposure in humans is the detection of PAH metabolites bound to albumin in blood. The potential of a similar PAH-albumin biomarker in Atlantic cod was therefore investigated by a desktop bioinformatic study followed by liquid chromatography mass spectrometry/mass spectrometry analysis of plasma from 16 fish. For the first time, an albumin-like protein in plasma of Atlantic cod is described, and the biomarker potential based on PAH-albumin adduct detection is discussed. Due to the detected low abundance of the albumin-like protein, it was found unlikely to be applicable as a new biomarker tool for evaluation of PAH exposure.

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“…Atlantic cod is an ecologically and economically important species in the North Atlantic Ocean, and has commonly been used as a bioindicator species in environmental monitoring programs [16][17][18] . The genome of Atlantic cod was published in 2011 19 , which has facilitated its increased use as model in toxicological studies [20][21][22][23][24] . Although these fish are all benthopelagic species, their natural habitats range from freshwater to marine environments, from tropical to temperate conditions, and reach sizes ranging from less than five cm (zebrafish and medaka) and 15 cm (killifish and stickleback) to 200 cm (Atlantic cod).…”

Section: Introductionmentioning

confidence: 99%

The Chemical Defensome of Fish: Conservation and Divergence of Genes Involved in Sensing and Responding to Pollutants Among Five Model Teleosts

Eide

Zhang

Karlsen

et al. 2021

Preprint

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How an organism copes with chemicals is largely determined by the genes and proteins that collectively function to defend against, detoxify and eliminate chemical stressors. This integrative network includes receptors and transcription factors, biotransformation enzymes, transporters, antioxidants, and metal- and heat-responsive genes, and is collectively known as the chemical defensome. Although the types of defensome genes are generally conserved in animals, there are important differences in the complement and function of specific genes between species. Teleost fish is the largest group of vertebrate species and can provide valuable insights into the evolution and functional diversity of defensome genes.In this study, we compared the genes comprising the chemical defensome of five fish species that span the teleosteii evolutionary branch often used as model species in toxicological studies and environmental monitoring programs: zebrafish (Danio rerio), Atlantic cod (Gadus morhua), medaka (Oryzias latipes), Atlantic killifish (Fundulus heteroclitus) and three-spined stickleback (Gasterosteus aculeatus). Genome miningrevealed evolved differences in the number and composition of defensome genes that can have implication for how these species sense and respond to environmental pollutants. The results indicate that knowledge regarding the diversity and function of the defensome will be important for toxicological testing and risk assessment studies.

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Single and mixture effects of aquatic micropollutants studied in precision-cut liver slices of Atlantic cod (Gadus morhua)

Cited by 14 publications

References 64 publications

Independent losses of a xenobiotic receptor across teleost evolution

Independent losses of a xenobiotic receptor across teleost evolution

Identification of an albumin-like protein in plasma of Atlantic cod ( Gadus morhua ) and its biomarker potential for PAH contamination

The Chemical Defensome of Fish: Conservation and Divergence of Genes Involved in Sensing and Responding to Pollutants Among Five Model Teleosts

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