Single ascospore detection for the forecasting of <i>Sclerotinia</i> stem rot of canola

Duarte, Pedro Augusto Silva; Menze, Lukas; Abdelrasoul, Gaser N.; Yosinski, Shari; Kobos, Zak; Stuermer, Riley; Reed, Mark A.; Yang, Jian; Li, Xiujie S.; Chen, Jie

doi:10.1039/d0lc00426j

Cited by 17 publications

(18 citation statements)

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“…Different types of spores have different dielectric properties, which in turn allows for their differentiation. 20 Thus, the frequency of the applied DEP signal could be easily tuned to allow for the selective capture of a specific type of spore within a mixed sample flowing through the device. Besides the selectivity based on the different dielectric properties, the microwells in our current device also provide a physical restriction to the size of particles that can be captured.…”

Section: Resultsmentioning

confidence: 99%

“…When compared to other approaches, 20 some key issues have been improved. Our current quantification method is based on static rather than dynamic impedance measurements, which increases the sensitivity and reduces the need for instruments with an ultrafast response time.…”

Section: Resultsmentioning

confidence: 99%

“…Nano- and micromachined devices emerge as an alternative to provide such a platform. 20 , 24 In this study, we designed a microfluidic device based on a nanoelectrode-activated microwell array for the capture and quantification of S. sclerotiorum spores. Microwell arrays have been commonly used for high-throughput cell sequencing 25 − 29 and cell pairing, 30 , 31 as well as for cancer cell identification and characterization.…”

Section: Introductionmentioning

confidence: 99%

“…Another popular strategy to assess the risk of SSR is through petal infestation measurements. The identification of spores in plant petals using the agar test is the most common approach. − The percentage of petals infested is then correlated to the risk of disease development . A major limitation of this method is the incubation time delay, which can be significant and especially harmful when weather conditions are favorable for SSR development .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Highly Efficient Capture and Quantification of the Airborne Fungal Pathogen Sclerotinia sclerotiorum Employing a Nanoelectrode-Activated Microwell Array

et al. 2021

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In this study, we present a microdevice for the capture and quantification of Sclerotinia sclerotiorum spores, pathogenic agents of one of the most harmful infectious diseases of crops, Sclerotinia stem rot. The early prognosis of an outbreak is critical to avoid severe economic losses and can be achieved by the detection of a small number of airborne spores. However, the current lack of simple and effective methods to quantify fungal airborne pathogens has hindered the development of an accurate early warning system. We developed a device that remedies these limitations based on a microfluidic design that contains a nanothick aluminum electrode structure integrated with a picoliter well array for dielectrophoresis-driven capture of spores and on-chip quantitative detection employing impedimetric sensing. Based on experimental results, we demonstrated a highly efficient spore trapping rate of more than 90% with an effective impedimetric sensing method that allowed the spore quantification of each column in the array and achieved a sensitivity of 2%/spore at 5 kHz and 1.6%/spore at 20 kHz, enabling single spore detection. We envision that our device will contribute to the development of a low-cost microfluidic platform that could be integrated into an infectious plant disease forecasting tool for crop protection.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Highly Efficient Capture and Quantification of the Airborne Fungal Pathogen Sclerotinia sclerotiorum Employing a Nanoelectrode-Activated Microwell Array

et al. 2021

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“…Another limitation these designs face is their inability to readily provide information about the number of sorted cells. Additional cell counting structures such as microfluidic Coulter counters, which have been shown to reliably detect single cells, − may be employed to count the number of cells flowing through an outlet channel at the cost of an increased overall system complexity. While combining cell capture and cell counting platforms have provided reliable results, each device is designed to solely perform its respective task.…”

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confidence: 99%

Selective Single-Cell Sorting Using a Multisectorial Electroactive Nanowell Platform

et al. 2021

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Current approaches in targeted patient treatments often require the rapid isolation of specific rare target cells. Stream-based dielectrophoresis (DEP) based cell sorters have the limitation that the maximum number of sortable cell types is equivalent to the number of output channels, which makes upscaling to a higher number of different cell types technically challenging. Here, we present a microfluidic platform for selective single-cell sorting that bypasses this limitation. The platform consists of 10 000 nanoliter wells which are placed on top of interdigitated electrodes (IDEs) that facilitate dielectrophoresis-driven capture of cells. By use of a multisectorial design formed by 10 individually addressable IDE structures, our platform can capture a large number of different cell types. The sectorial approach allows for fast and straightforward modification to sort complex samples as different cell types are captured in different sectors and therefore removes the need for individual output channels per cell type. Experimental results obtained with a mixed sample of benign (MCF-10A) and malignant (MDA-MB-231) breast cells showed a target to nontarget sorting accuracy of over 95%. We envision that the high accuracy of our platform, in addition to its versatility and simplicity, will aid clinical environments where reliable sorting of varying complex samples is essential.

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Low-cost sheath-less microfluidic impedance cytometry for point-of-care applications

Hosseini,

Bahrami,

Zolghadr

et al. 2023

Microfluid Nanofluid

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Single ascospore detection for the forecasting of Sclerotinia stem rot of canola

Abstract: Smart-agriculture technologies comprise a set of management systems designed to sustainably increase the efficiency and productivity of farming. In this paper, we present a lab-on-a-chip device that can be employed...

Cited by 17 publications

References 45 publications

Highly Efficient Capture and Quantification of the Airborne Fungal Pathogen Sclerotinia sclerotiorum Employing a Nanoelectrode-Activated Microwell Array

Highly Efficient Capture and Quantification of the Airborne Fungal Pathogen Sclerotinia sclerotiorum Employing a Nanoelectrode-Activated Microwell Array

Selective Single-Cell Sorting Using a Multisectorial Electroactive Nanowell Platform

Low-cost sheath-less microfluidic impedance cytometry for point-of-care applications

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