Single-Atom Cobalt-Based Electrochemical Biomimetic Uric Acid Sensor with Wide Linear Range and Ultralow Detection Limit

Abstract

Uric acid (UA) detection is essential in diagnosis of arthritis, preeclampsia, renal disorder, and cardiovascular diseases, but it is very challenging to realize the required wide detection range and low detection limit. We present here a single-atom catalyst consisting of Co(II) atoms coordinated by an average of 3.4 N atoms on an N-doped graphene matrix (A–Co–NG) to build an electrochemical biomimetic sensor for UA detection. The A–Co–NG sensor achieves a wide detection range over 0.4–41,950 μM and an extremely low detection limit of 33.3 ± 0.024 nM, which are much better than previously reported sensors based on various nanostructured materials. Besides, the A–Co–NG sensor also demonstrates its accurate serum diagnosis for UA for its practical application. Combination of experimental and theoretical calculation discovers that the catalytic process of the A–Co–NG toward UA starts from the oxidation of Co species to form a Co3+–OH–UA*, followed by the generation of Co3+–OH + *UA_H, eventually leading to N–H bond dissociation for the formation of oxidized UA molecule and reduction of oxidized Co3+ to Co2+ for the regenerated A–Co–NG. This work provides a promising material to realize UA detection with wide detection range and low detection limit to meet the practical diagnosis requirements, and the proposed sensing mechanism sheds light on fundamental insights for guiding exploration of other biosensing processes.

Graphene and its Derivatives for Bone Tissue Engineering: In Vitro and In Vivo Evaluation of Graphene-Based Scaffolds, Membranes and Coatings

“…Research on graphene-based nanomaterials has boomed in biomaterial applications over the past few years. With outstanding physical, chemical and biological properties, graphene is considered to be revolutionary material and shows great potential for applications in tissue regeneration, drug delivery and other biomedical areas (Hu et al, 2020;Fu et al, 2021;Unal et al, 2021). Therefore, the purpose of this review is to highlight the scientific progress over the years and further summarize the physical and chemical properties, family members and applications in bone tissue engineering of this graphene-based nanomaterial.…”

Single‐Atom Nanozymes for Biomedical Applications: Recent Advances and Challenges

“…Finally, in terms of application, a sensitive detection method for H 2 O 2 , glucose, and AA was developed with the optimized Cu-NC-700 SAzymes. Hongye FeÀ SSN [14] Fe [15] RuÀ AlaÀ C 3 N 4 [16] AÀ CoÀ NG [17] FeÀ NÀ C [18] Apt/FeÀ NÀ C [19] FeÀ NÀ C [20] Pt/NiCo-LDH [21] FeÀ NÀ C [22] CoÀ MoS 2 [23] FeÀ N/C [24] CoÀ NÀ C [25] Biomedical therapy Cancer…”

“…Although several nanomaterials have been reported for the electrochemical detection of UA, such as Prussian blue (PB)/nitrogen-doped carbon nanotubes (CNTs) [80] and gold nanoparticles loaded on graphene oxide (GO), [81] the defect of the low active site leads to their poor sensing performance to achieve high sensitivity, which is solved by the highly dispersed active sites of SAzymes. Fang Xinyu et al [17] reported a uric acid bionanosensing detection system (AÀ CoÀ NG) designed with cobalt-based SAzymes, whose principle reaction is: oxidation of Co generates Co 3 + À OH À . The redox reaction provides the conditions for electrochemical sensing, and the cobalt atoms of this experimental design are densely distributed in the The SAzymes system (AÀ CoÀ NG) with N-doped graphene body that has high enzyme-like electron sensing activity.…”

Folic Acid Self-Assembly Enabling Manganese Single-Atom Electrocatalyst for Selective Nitrogen Reduction to Ammonia

“…However, the thermodynamically unstable nature of single metal atoms poses challenges for preparing stable SACs. To successfully engineer SACs, suitable precursors (including metal and supporting materials), effective synthetic strategies and intriguing metalsupport interactions are three important considerations, which are also intimately correlated with the exotic geometric and electronic structures of SACs [22,24,25]. As a representative, Mn single-atom catalysts (Mn SACs) with Mn δ+ -N x sites have been developed and proved to be highly active for CO 2 reduction and oxygen reduction; however, they have seldom been explored for NRR [26][27][28][29][30].…”