Single-atom cobalt catalysts as highly efficient oxidase mimics for time-based visualization monitoring the TAC of skin care products

Natural Enzyme-Inspired Design of the Single-Atom Cu Nanozyme as Dual-Enzyme Mimics for Distinguishing Total Antioxidant Capacity and the Ascorbic Acid Level

“…In the inhibition of TMB oxidation caused by AA, the reactive oxygen generated by the catalyst was more likely to react with AA, leading to the delay of TMB oxidation. Nevertheless, GSH and Cys exhibited different inhibition mechanisms for TMB oxidation with AA (Figure b–d), and sulfhydryl groups on GSH and Cys could poison the active site, leading to an irreversible decrease in activity …”

“…Nevertheless, GSH and Cys exhibited different inhibition mechanisms for TMB oxidation with AA (Figure 5b−d), and sulfhydryl groups on GSH and Cys could poison the active site, leading to an irreversible decrease in activity. 51 Then, the POD mimic activity of Cu-N/C was used to detect TAC in fruits, beverages, and vitamin C tablets and compared with the standard DPPH method (Figure S21). 52−54 The results obtained by the two methods were close to each other, indicating that this method had high credibility (Figure 5e).…”

Enzyme Mimetic Activity of Intercalated Pd–H Nanosheets for Bioanalysis

“…Macromolecular biocatalysts, named enzymes found in living organisms and mainly composed of proteins, are responsible for regulating the possible chemical reactions in living organisms . Owing to their high substrate specificity and catalytic activity, natural enzymes have been extensively used in biological fields, industry, medicines, etc. − However, their low stability, high cost, and complex production process increase the demand for artificial enzymes, which are proven to be promising candidates in the fields of nanotechnology, , biotechnology, , and nanomaterials science. , Enzyme-like activities of nanozymes, such as oxidase-like, − peroxidase-like, − and catalase-like, , have been explored through various studies. Furthermore, nanozymes have been explored for the antibacterial activity at physiological pH in the presence of adenosine triphosphate (ATP) .…”

Catalytic Activation of Molecular Oxygen Toward Producing Hydroxyl Radicals Controllably for Highly Selective Oxidation of Hydroxyl Compounds under Mild Conditions

“…16 By density functional theory (DFT) calculation and activity analysis experiments, the oxidase (OXD)-like activity has been demonstrated to depend largely on the local N coordination environment around the Co sites such as Co−N 4 . 17 Even more interestingly, when some peroxidase-like nanomaterials combine with natural oxidases, a cascade catalysis may be created to enable the generation of OH • via promoting the interconversion among ROS. For example, H 2 O 2 could be initially produced from O 2 by using glucose oxidase, and subsequently, H 2 O 2 is decomposed into OH • catalyzed with the CuO-based artificial enzyme.…”

“…Recently, the great advancement achieved with certain nanomaterial-based artificial enzymes has encouraged the development in biomimetic activation of O 2 into various ROS. , It is noteworthy that inspired by the metal coordination structure of natural enzymes, some biomimetic single-atom nanomaterials such as the ZIF-8-derived Zn–N–C can mimic the activity of peroxidase (POD) to facilely yield OH • from H 2 O 2 decomposition at 25 °C, overcoming the disadvantages of native enzymes such as structural vulnerability to damage, narrow pH value scale, etc . By density functional theory (DFT) calculation and activity analysis experiments, the oxidase (OXD)-like activity has been demonstrated to depend largely on the local N coordination environment around the Co sites such as Co–N 4 . Even more interestingly, when some peroxidase-like nanomaterials combine with natural oxidases, a cascade catalysis may be created to enable the generation of OH • via promoting the interconversion among ROS.…”