2021
DOI: 10.1016/j.immuni.2021.02.018
|View full text |Cite
|
Sign up to set email alerts
|

Single-cell analysis by mass cytometry reveals metabolic states of early-activated CD8+ T cells during the primary immune response

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

7
86
0

Year Published

2021
2021
2024
2024

Publication Types

Select...
6
1

Relationship

1
6

Authors

Journals

citations
Cited by 88 publications
(93 citation statements)
references
References 79 publications
7
86
0
Order By: Relevance
“…Importantly, aside from capturing the known changes in these pathways between the naïve and activated endpoint states, our data further provide a dynamic description of these changes ( Figure 2B ). In line with this, our results recapitulate recent observations from in vivo 13 C-labeling (Ma et al, 2019) and in vivo single-cell mass-cytometry data (Levine et al, 2021) demonstrating that, contrary to the widespread notion, both glycolysis and OXPHOS are important in activated CD8 + T cells in vivo , with OXPHOS also being rapidly upregulated during early activation (Levine et al, 2021) ( Figure 2B ), and glycolysis taking over only at later activation stages (but not at the expense of OXPHOS activity) ( Figure 2B ). In summary, our in vitro pathway analysis strategy is able to identify known aspects of the in vivo metabolic rewiring undergone by CD8 + T cells during activation, while further providing a novel dynamic description of this rewiring.…”
Section: Resultssupporting
confidence: 91%
See 3 more Smart Citations
“…Importantly, aside from capturing the known changes in these pathways between the naïve and activated endpoint states, our data further provide a dynamic description of these changes ( Figure 2B ). In line with this, our results recapitulate recent observations from in vivo 13 C-labeling (Ma et al, 2019) and in vivo single-cell mass-cytometry data (Levine et al, 2021) demonstrating that, contrary to the widespread notion, both glycolysis and OXPHOS are important in activated CD8 + T cells in vivo , with OXPHOS also being rapidly upregulated during early activation (Levine et al, 2021) ( Figure 2B ), and glycolysis taking over only at later activation stages (but not at the expense of OXPHOS activity) ( Figure 2B ). In summary, our in vitro pathway analysis strategy is able to identify known aspects of the in vivo metabolic rewiring undergone by CD8 + T cells during activation, while further providing a novel dynamic description of this rewiring.…”
Section: Resultssupporting
confidence: 91%
“…Our data further captured the known higher reliance on OXPHOS of Tcm vs. Tem cells (van der Windt et al, 2012), and supported recent observations showing that long-lived Tcm cells rely on intrinsic FAS to fuel their FAO (Cui et al, 2015; O’Sullivan et al, 2014) ( Figure S2A ). Notably, our data also suggest that, much like during activation both glycolysis and OXPHOS are upregulated, with glycolysis taking over only upon full activation (Levine et al, 2021), the downregulation of glycolysis in the transition to a memory phenotype seems to be concomitant with a downregulation in OXPHOS, with OXPHOS only becoming dominant for fully differentiated ( Tcm/Tem ) memory cells ( Figure S2A ). In this sense, the sustained glycolytic activity observed in the memory precursors of our effector-polarized sample ( Tcm p /Tem p ) ( Figure S2A ) may partly be due to the presence of IL-2 stimulation, in agreement with the known role of the latter in promoting glycolysis (Bauer et al, 2004).…”
Section: Resultsmentioning
confidence: 53%
See 2 more Smart Citations
“…Besides transcriptional characterization, cellular metabolomics at the single-cell level defined the rare early activated CD8+T-cells in a primary immune response [96]. A mass cytometry approach revealed that early activated T-cells exhibited simultaneous peaks in both glycolysis and OXPHOS, which are distinct from naive and committed T-cells.…”
Section: Potential Biomarkers Of Clinical Response In Icimentioning
confidence: 99%