Single-cell analysis: Understanding infected cell heterogeneity

Alberdi, Lucrecia; Méresse, Stéphane

doi:10.1080/21505594.2016.1253659

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…Though, gene expression analysis of intracellular bacteria is usually measured at the whole population level, which may mask potential cell-to-cell heterogeneity. 59,60 Furthermore, a number of data exist from the comparison of the bacteria growing in LB broth, or a rich media, with those obtained from the intracellular environment, this may also affect the relative conclusions obtained from the analysis. 61 In this work we used fluorescence reporter fusions to analyze the relevance of different metabolic pathways used by Salmonella during infections in RAW 264.7 macrophages.…”

Section: Discussionmentioning

confidence: 99%

The infectious intracellular lifestyle ofSalmonella entericarelies on the adaptation to nutritional conditions within theSalmonella-containing vacuole

et al. 2016

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Salmonella enterica serovar Typhimurium (S. Typhimurium) is a Gram-negative pathogen that causes various host-specific diseases. During their life cycle, Salmonellae survive frequent exposures to a variety of environmental stresses, e.g. carbon-source starvation. The virulence of this pathogen relies on its ability to establish a replicative niche, named Salmonella-containing vacuole, inside host cells. However, the microenvironment of the SCV and the bacterial metabolic pathways required during infection are largely undefined. In this work we developed different biological probes whose expression is modulated by the environment and the physiological state of the bacterium. We constructed transcriptional reporters by fusing promoter regions to the gfpmut3a gene to monitor the expression profile of genes involved in glucose utilization and lipid catabolism. The induction of these probes by a specific metabolic change was first tested in vitro, and then during different conditions of infection in macrophages. We were able to determine that Entner-Doudoroff is the main metabolic pathway utilized by Salmonella during infection in mouse macrophages. Furthermore, we found sub-populations of bacteria expressing genes involved in pathways for the utilization of different sources of carbon. These populations are modified in presence of different metabolizable substrates, suggesting the coexistence of Salmonella with diverse metabolic states during the infection.

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Section: Discussionmentioning

confidence: 99%