Single-Cell Approach Reveals Intercellular Heterogeneity in Phage-Producing Capacities

Kannoly, Sherin; Oken, Gabriella; Shadan, Jonathan; Musheyev, David; Singh, Kevin; Singh, Abhyudai; Dennehy, John J.

doi:10.1128/spectrum.02663-21

Cited by 9 publications

(8 citation statements)

References 51 publications

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“…In order to find a robust feature that is less dependent on the specific choice of the parameters, we focused on the features robustly observed for a large range of f τ and f β . However, in reality, f τ and f β should positively correlate [5,13].…”

Section: Discussionmentioning

confidence: 95%

“…1. In reality, a positive correlation between the increase of the latency time and the increase of the burst size has been observed [5,13].…”

Section: M1 -Simple Model Of Linmentioning

confidence: 98%

“…1. In reality, a positive correlation between the increase of the latency time and the increase of the burst size has been observed [5, 13]. However, in the present analysis, we control the two parameters independently to allow the model to explore the pros and cons of LIN fully.…”

Section: Modelsmentioning

confidence: 99%

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Competitive advantages of T-even phage lysis inhibition in response to secondary infection

Hvid,

Mitarai

2024

Preprint

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T-even bacteriophages are known to employ lysis inhibition (LIN), where the lysis of an infected host is delayed in response to secondary adsorptions. Upon the eventual burst of the host, significantly more phage progenies are released. Here, we analysed the competitive advantage of LIN using a mathematical model. In batch culture, LIN provides a bigger phage yield at the end of the growth and, in addition, gives a competitive advantage against LIN mutants with rapid lysis by letting them adsorb to already infected hosts in the LIN state. By simulating plaque formation in a spatially structured environment, we show that, while LIN phages will produce a smaller zone of clearance, the area over which the phages spread is actually comparable to those without LIN. The analysis suggests that LIN induced by secondary adsorption is favourable in terms of competition, both in spatially homogeneous and inhomogeneous environments.

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Section: Discussionmentioning

confidence: 95%

“…1. In reality, a positive correlation between the increase of the latency time and the increase of the burst size has been observed [5,13].…”

Section: M1 -Simple Model Of Linmentioning

confidence: 98%

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Competitive advantages of T-even phage lysis inhibition in response to secondary infection

Hvid,

Mitarai

2024

Preprint

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“…However, more work is needed to identify the mechanisms underlying variability, including those that impact other viral traits. For example, while correlations between latent period and burst size have been observed, with phage strains with longer latent periods having larger burst sizes [13], recent studies suggest that variation in (induced) lysis time does not contribute to observed burst size variability [46,47]. Caution in the interpretation of multi-cycle response curves is required, as viral traits often depend on host growth rate which can vary in experimental timescales [12].…”

Section: Discussionmentioning

confidence: 99%

Accounting for Cellular-Level Variation in Lysis: Implications for Virus-Host Dynamics

Dominguez-Mirazo,

Harris,

Demory

et al. 2024

Preprint

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Viral impacts on microbial populations depend on interaction phenotypes - including viral traits spanning adsorption rate, latent period, and burst size. The latent period is a key viral trait in lytic infections. Defined as the time from viral adsorption to viral progeny release, the latent period of bacteriophage is conventionally inferred via one-step growth curves in which the accumulation of free virus is measured over time in a population of infected cells. Developed more than 80 years ago, one-step growth curves do not account for cellular-level variability in the timing of lysis, potentially biasing inference of viral traits. Here, we use nonlinear dynamical models to understand how individual-level variation of the latent period impacts virus-host dynamics. Our modeling approach shows that inference of latent period via one-step growth curves is systematically biased - generating estimates of shorter latent periods than the underlying population-level mean. The bias arises because variability in lysis timing at the cellular level leads to a fraction of early burst events which are interpreted, artefactually, as an earlier mean time of viral release. We develop a computational framework to estimate latent period variability from joint measurements of host and free virus populations. Our computational framework recovers both the mean and variance of the latent period within simulated infections including realistic measurement noise. This work suggests that reframing the latent period as a distribution to account for variability in the population will improve the study of viral traits and their role in shaping microbial populations.

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“…The study found a positive correlation between lytic activity and burst size, while a negative correlation was observed between lytic activity and both the latent phase and the rising phase. The magnitude of the burst size holds significant importance as it directly correlates with the quantity of viral particles generated, hence indicating the possibility for further cellular infections (Kannoly et al, 2023). After conducting the single-step experiment, it was observed that the lytic Psa phages had a growth curve with an Sshape, which was distinguished by a substantial burst size and a brief latency period.…”

Section: Lytic Activitymentioning

confidence: 99%

Harnessing the bacteriophages to deal with canker infection of kiwi fruit: challenges and future perspective

Raza,

Hassan,

Juniad

et al. 2024

Adv. Virology

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The worldwide kiwifruit industry has suffered significant economic losses in recent decades as a result of the destructive bacterial plant disease Pseudomonas syringae pv. actinidiae (Psa). Existing control approaches, which depend on the use of copper bactericides and antibiotics, are facing growing challenges due to the rise of resistance to antibiotics and ecological issues. Although biocontrol techniques show promise in laboratory settings, their efficacy in real-world field situations remains unclear. In order to tackle this issue, the emergence of a phage-based biocontrol method becomes a vital alternative, considering the precise targeting of bacteriophages (phages) towards the particular bacteria and their ecologically benign characteristics. This thorough assessment commences by delineating the repercussions of Psa-induced kiwifruit canker, underscoring the need for pioneering management strategies. The text explores the many types and strengths of Psa strains, and then shifts its attention to recent progress in the identification and description of Psa phages. The main topics discussed are the physical structure of phages, the spectrum of organisms they may infect, their ability to destroy bacterial cells, the study of their genetic material, and the process by which they break down bacterial cells. The review examines biocontrol tactics and their possible obstacles in kiwifruit orchards, specifically focusing on abiotic variables such as elevated temperature, UV irradiation and severe pH. The manuscript highlights the crucial importance of phages in efficiently controlling Psa infections, providing a sustainable and focused approach for safeguarding plants.

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Single-Cell Approach Reveals Intercellular Heterogeneity in Phage-Producing Capacities

Abstract: Phages produce offspring by hijacking a cell's replicative machinery. Previously, it was noted that the variation in the number of phages produced by single infected cells far exceeded cell size variation.

Cited by 9 publications

References 51 publications

Competitive advantages of T-even phage lysis inhibition in response to secondary infection

Competitive advantages of T-even phage lysis inhibition in response to secondary infection

Accounting for Cellular-Level Variation in Lysis: Implications for Virus-Host Dynamics

Harnessing the bacteriophages to deal with canker infection of kiwi fruit: challenges and future perspective

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