Single cell-asymmetrical flow field-flow fractionation/ICP-time of flight-mass spectrometry (sc-AF4/ICP-ToF-MS): an efficient alternative for the cleaning and multielemental analysis of individual cells

Chronakis, Michail Ioannis; Au, Marcus von der; Meermann, Björn

doi:10.1039/d2ja00264g

Cited by 6 publications

(3 citation statements)

References 59 publications

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“…This can for example be achieved by coupling asymmetric flow-field flow fractionation multiangle laser light scattering (AF 4 -MALS) to SP ICP-ToF-MS which can provide additional and complementary perspectives on particle composition and size. Previously, AF 4 -MALS-SP ICP-ToF-MS has been applied to investigate individual cells 14 and engineered metallic nanoparticles 15 and has the capability to provide new insights into the Hg metabolism of cetaceans.…”

Section: Introductionmentioning

confidence: 99%

AF₄-MALS-SP ICP-ToF-MS analysis gives insight into nature of HgSe nanoparticles formed by cetaceans

Paton,

Moro,

Lockwood

et al. 2024

Environ. Sci.: Nano

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Section: Introductionmentioning

confidence: 99%

AF₄-MALS-SP ICP-ToF-MS analysis gives insight into nature of HgSe nanoparticles formed by cetaceans

Paton,

Moro,

Lockwood

et al. 2024

Environ. Sci.: Nano

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“…This can for example be achieved by coupling asymmetric flow-field flow fractionation multiangle laser light scattering (AF4-MALS) to SP ICP-ToF-MS which can provide additional and complementary perspectives on particle composition and size. Previously, AF4-MALS-SP ICP-ToF-MS has been applied to investigate individual cells [14] and engineered metallic nanoparticles [15] and has the capability to provide new insights into the Hg metabolism of cetaceans.…”

Section: Introductionmentioning

confidence: 99%

AF4-MALS-SP ICP-ToF-MS analysis gives insight into nature of HgSe nanoparticles formed by cetaceans.

Paton,

Thaíse Moro,

Lockwood

et al. 2024

Preprint

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Cetaceans are known to accumulate very high levels of mercury (Hg) and unlike fish species, most of the mercury is not present as methylmercury (MeHg) but instead as inorganic Hg. It has been suggested this is a result of a detoxification process which involves the sequestering of Hg by Se-containing proteins, concluding with the formation of HgSe nanoparticles. However, their elemental compositions besides Hg and Se, as well as their genesis are unknown. We applied asymmetric flow-field flow fractionation - multiangle laser light scattering (AF4 -MALS) coupled to single particle inductively coupled plasma - time of flight - mass spectrometry (SP ICP-ToF-MS) for the size-dependant separation, counting and further elemental characterisation of of Hg/Se-containing nanoparticles in the liver and cerebellum of a sperm whale. Using a proteinase K digestion method, we isolated and identified particles which had size-independent masses of Hg and as well as a continuous molar ratio of Hg to Se. We also identified Cd and Sn on particles alongside Hg and Se, which indicated the detoxification process resulting in Hg/Se nanoparticles may not be specific to MeHg. This data indicates nanoclusters of toxic elements, bound to selenium, make up a nanoparticle core which is surrounded by a larger non-metal(loid) corona.

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“…Emerging ICP-time of flight (TOF)-MS can address these challenges by simultaneously monitoring the full mass spectra of individual cells, as demonstrated in a few proof-of-principle studies using simple unicellular organisms as model analytes. [19][20][21][22] Nevertheless, for animal cells represented by human sperm cells with poorly understood compositions, small sample amounts, and complex biological matrices, the quantitative analytical procedure and data analysis method for their single-cell multi-element analysis still remain underdeveloped. In this study, we firstly established a pretreatment procedure for single-cell multi-element analysis of human sperm cells (Text S1-S3, Fig.…”

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confidence: 99%

Single-cell multi-element analysis reveals element distribution pattern in human sperm

Tian

Liu³

et al. 2023

Chem. Commun.

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Single cell-asymmetrical flow field-flow fractionation/ICP-time of flight-mass spectrometry (sc-AF4/ICP-ToF-MS): an efficient alternative for the cleaning and multielemental analysis of individual cells

Abstract: One of the greatest challenges in the analysis of single cells, quite often is the severe ionic background their samples exhibit. With that in mind, Asymmetrical Flow Field-Flow Fractionation (AF4),...

Cited by 6 publications

References 59 publications

AF₄-MALS-SP ICP-ToF-MS analysis gives insight into nature of HgSe nanoparticles formed by cetaceans

AF₄-MALS-SP ICP-ToF-MS analysis gives insight into nature of HgSe nanoparticles formed by cetaceans

AF4-MALS-SP ICP-ToF-MS analysis gives insight into nature of HgSe nanoparticles formed by cetaceans.

Single-cell multi-element analysis reveals element distribution pattern in human sperm

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