Single-Cell Electric Lysis on an Electroosmotic-Driven Microfluidic Chip with Arrays of Microwells

Abstract

Accurate analysis at the single-cell level has become a highly attractive tool for investigating cellular content. An electroosmotic-driven microfluidic chip with arrays of 30-μm-diameter microwells was developed for single-cell electric lysis in the present study. The cellular occupancy in the microwells when the applied voltage was 5 V (82.4%) was slightly higher than that at an applied voltage of 10 V (81.8%). When the applied voltage was increased to 15 V, the cellular occupancy in the microwells dropped to 64.3%. More than 50% of the occupied microwells contain individual cells. The results of electric lysis experiments at the single-cell level indicate that the cells were gradually lysed as the DC voltage of 30 V was applied; the cell was fully lysed after 25 s. Single-cell electric lysis was demonstrated in the proposed microfluidic chip, which is suitable for high-throughput cell lysis.

Microfluidic Sample Preparation for Single Cell Analysis

“…Similarly, another group designed a microfluidic chip to load single cells into 30 µm diameter wells via electroosmotic driven flow and then lyse the cells under a 30 V potential. 211 …”

Evaluation of Mechanical Tearing based Cell Disruption Capability to Shape Nanostructures formed on Nanoporous Alumina Filter

“…화학 적 세포파쇄 방법은 일반적으로 세포 용해액을 통해 세포 외피 및 세포핵을 화학적으로 분해시켜 세포를 파쇄 하지만 과정이 복잡하 며, 용해액 이외의 잔여물이 발생하고, 용해 과정 중 DNA와 단백 질 등이 오염될 수 있는 단점을 가지고 있다 [2,3] . 전기적 세포파쇄 방법으로는 전기장을 이용하여 세포를 파쇄 하는 방법이 있으며, 세포파쇄에 필요한 1 kV/cm 이상의 높은 전기장을 얻기 위한 외 부 전기장치가 필요하고, LOC(lap on a chip)을 위한 소규모 파쇄 에 적합하다 [1,4] . 기계적 세포파쇄 방법으로는 마이크로 비드에 의 한 충격이나 블레이드 회전에 의한 전단력으로 세포를 파쇄 [6,7] .…”

Electrical lysis of cells for detergent-free droplet assays

“…Lysis of cells can be achieved using chemical (e.g., detergents), 9,10 optical (e.g., pulsed laser), 23 mechanical (e.g., nanoknives), 24 acoustic (e.g., sonication), 25 or electrical 26 techniques. In droplet microfluidics, robust lysis is most commonly achieved using proteases and detergents that digest proteins and solubilize cellular lipids 9,10 .…”