Single Cell Fluorescence Imaging to Investigate Calcium Signaling in Primary Cultured Neurones

Pocock, Jennifer M.; Evans, Gareth J.

doi:10.1007/978-94-010-0688-0_41

Cited by 1 publication

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“…We imaged the responses of microglia to albumin and ATP in co‐culture with CGC neurones, which do not respond to either albumin or ATP but do show a large increase in [Ca 2+ ] c following KCl stimulation. The KCl‐induced Ca 2+ response in CGC neurones occurs via the opening of voltage‐dependent calcium channels (Pocock and Evans 2000) and thus, serves as a positive control for Mn 2+ entry via Ca 2+ ‐permeant channels. Neurones were identified by their morphology, low basal [Ca 2+ ] c and responses to KCl, and microglia by subsequent staining with isolectin B4.…”

Section: Effect Of Albumin or Fraction V On Intracellular Free Calciumentioning

confidence: 99%

Pure albumin is a potent trigger of calcium signalling and proliferation in microglia but not macrophages or astrocytes

Hooper

Taylor

Pocock

2005

Journal of Neurochemistry

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Microglial activation is implicated in the neurotoxicity of neurodegenerative diseases. Raised intracerebral levels of albumin are associated with the pathology of Alzheimer's disease, multiple sclerosis, and stroke where blood-brain barrier damage is evident. We report here that treatment of primary cultured microglia and the N9 microglial cell line with pure albumin, or albumin in which fatty acids and immunoglobulins remain attached (fraction V), induced a rise in intracellular calcium. This rise in intracellular calcium was mediated via Src tyrosine kinase and phospholipase C. The albumininduced calcium response was coupled to microglial proliferation, which was prevented by BAPTA, U73122 or PP2 but not mimicked by thapsigargin. In contrast, peritoneal macrophages were resistant to albumin-or fraction V-induced calcium responses and proliferation, whilst primary cultured astrocytes or the TSA-3 astrocyte cell line were responsive to fraction V albumin but not pure albumin. Furthermore, cerebellar granule neurones did not respond to albumin. These data suggest that albumin may play a role in microglial activation in pathological situations involving blood-brain barrier impairment, and that the specific responses of microglia to albumin allow a distinction to be made between the signalling responses of microglia, blood-borne macrophages, astrocytes and neurones.

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Section: Effect Of Albumin or Fraction V On Intracellular Free Calciumentioning

confidence: 99%

Pure albumin is a potent trigger of calcium signalling and proliferation in microglia but not macrophages or astrocytes

Hooper

Taylor

Pocock

2005

Journal of Neurochemistry

Self Cite

View full text Add to dashboard Cite

show abstract

Single Cell Fluorescence Imaging to Investigate Calcium Signaling in Primary Cultured Neurones

Cited by 1 publication

References 32 publications

Pure albumin is a potent trigger of calcium signalling and proliferation in microglia but not macrophages or astrocytes

Pure albumin is a potent trigger of calcium signalling and proliferation in microglia but not macrophages or astrocytes

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