Single-cell force spectroscopy as a technique to quantify human red blood cell adhesion to subendothelial laminin

Maciaszek, Jamie L.; Partola, Kostyantyn; Zhang, Jing; Andemariam, Biree; Lykotrafitis, George

doi:10.1016/j.jbiomech.2014.10.016

Cited by 19 publications

(15 citation statements)

References 45 publications

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“…When an AFM indenter approaches a cell, the molecules (atoms) of the two surfaces interact mainly by Van Der Waals forces and hence the adhesion force is inevitable in cell-indenter interaction. This adhesion force has been studied by many researchers (Zhang and Zhang, 2008, Sirghi et al, 2008, Maciaszek et al, 2014. Therefore, it is desirable to take cell adhesion into consideration for the determination of its mechanical properties in cell AFM indentation.…”

Section: Theoretical Modelmentioning

confidence: 99%

“…As a result, adhesion forces are inevitably associated with performance in atomic force microscopy (AFM) indentation which in turn provides a method to quantitatively study cell adhesion (Alessandrini and Facci, 2005;Maciaszek et al, 2014). The work of adhesion (regarded as magnitude of energy needed per unit area for new surface) has been used to characterize cell adhesion (Sirghi et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of work of adhesion of biological cell under AFM bead indentation

Zhu

Siamantouras

Liu

et al. 2016

Journal of the Mechanical Behavior of Biomedical Materials

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Section: Theoretical Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Determination of work of adhesion of biological cell under AFM bead indentation

Zhu

Siamantouras

Liu

et al. 2016

Journal of the Mechanical Behavior of Biomedical Materials

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“…Applying lateral forces to characterize cell detachment forces by AFM has been investigated in numerous studies. They used the back of cantilevers to detach cells and characterize detachment forces and completely ignored the tip of probe [14,16].…”

Section: Introductionmentioning

confidence: 99%

Cellular Shear Adhesion Force Measurement and Simultaneous Imaging by Atomic Force Microscope

Hou

Wang

et al. 2017

J. Med. Biol. Eng.

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This paper presents a sensitive and fast cellular shear adhesion force measurement method using an atomic force microscope (AFM). In the work, the AFM was used both as a tool for the imaging of cells on the nano-scale and as a force sensor for the measurement of the shear adhesion force between the cell and the substrate. After the cell imaging, the measurement of cellular shear adhesion forces was made based on the different positions of the cell on the nano-scale. Moreover, different pushing speeds of probe and various locations of cells were used in experiments to study their influences. In this study, the measurement of the cell adhesion in the upper portion of the cell is different from that in the lower portion. It may reveal that the cancer cells have the metastasis tendency after cultured for 16 to 20 hours, which is significant for preventing metastasis in the patients diagnosed with early cancer lesions. Furthermore, the cellular shear adhesion forces of two types of living cancer cells were obtained based on the measurements of AFM cantilever deflections in the torsional and vertical directions. The results demonstrate that the shear adhesion force of cancer cells is twice as much as the same type of cancer cells with TRAIL. The method can also provide a way for the measurement of the cellular shear adhesion force between the cell and the substrate, and for the simultaneous exploration of cells using the AFM imaging and manipulation.2

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“…The atomic force microscope (AFM) nanoindentation can offer an accurate mechanical measurement of individual living cells [7][8][9]. The adhesion phenomenon, characterized as negative force in the experimental force-displacement curves obtained in AFM nanoindentation, was widely reported over the last two decades [10][11][12][13]. The adhesion behavior of cells with other nanoparticles is crucial for the biocompatibility of implants [14].…”

Section: Introductionmentioning

confidence: 99%

Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nano-indentation

Zhu

Liu

Zhang

et al. 2015

2015 International Conference on Manipulation, Manufacturing and Measurement on the Nanoscale (3m-Nano)

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In this study, we presented an investigation of mechanical properties by AFM nanoindentation on human hepatocellular carcinoma cells treated with fullerenol for 24, 48 and 72 h. AFM nanoindentation was routinely applied to investigate the morphology and biomechanical properties of living carcinoma cells, and adhesion phenomena (negative force) were detected in the obtained force-displacement curves. Conventionally, Hertz contact model has been widely used for determination of cell elasticity, however this contact model cannot account for adhesion. Alternatively, JKR contact model, as expected for adhesion circumstance, has been applied to fit the obtained force-displacement curves. In this investigation, we have derived both the work of adhesion and the elastic modulus of biological cells (human hepatocellular carcinoma) under fullerenol treatment. The results show that the chosen JKR model can provide better fitting results than Hertz contact model. The results show that both Young's modulus and work of adhesion exhibit significant variation as the treatment time increases. The calculated mechanical properties of elastic modulus and work of adhesion can be used as an effective bio-index to evaluate the effects of fullerenol or other anticancer agents on cancer cells and thus to provide insight into cancer progression in the treatment.

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Single-cell force spectroscopy as a technique to quantify human red blood cell adhesion to subendothelial laminin

Cited by 19 publications

References 45 publications

Determination of work of adhesion of biological cell under AFM bead indentation

Determination of work of adhesion of biological cell under AFM bead indentation

Cellular Shear Adhesion Force Measurement and Simultaneous Imaging by Atomic Force Microscope

Investigation of work of adhesion of biological cell (human hepatocellular carcinoma) by AFM nano-indentation

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