Single cell full-length transcriptome of human subcutaneous adipose tissue reveals unique and heterogeneous cell populations

Whytock, Katie L; Sun, Yifei; Divoux, Adeline; Smith, Steven R.; Walsh, Martin J.; Sparks, Lauren M.

doi:10.1016/j.isci.2022.104772

Cited by 29 publications

(22 citation statements)

References 64 publications

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“…Historically, unilocular adipocytes were considered to be uniform in both form and function; however, we now appreciate that this somewhat simplistic view is likely inaccurate. Multiple snRNAseq studies have identified adipocyte subpopulations within murine and human WAT depots [ 12 ▪▪ , 14 , 15 ▪▪ , 16 ▪ , 17 ▪ ] (Table 1 ). In line with earlier findings identifying the presence of distinct adipocyte subpopulations in epididymal WAT from lean and obese male mice [ 14 ], human abdominal SAT was also found to predominantly consist of two distinct subclasses of adipocytes [ 15 ▪▪ ].…”

Section: The Existence Of White Adipocyte Subpopulationsmentioning

confidence: 99%

“…A recent study has demonstrated that the quality of adipocyte transcriptomic profiling may be improved by using full-length snRNAseq, which results in an enhanced gene coverage that may permit more accurate profiling and identification of adipocyte subtypes within WAT [ 16 ▪ ]. This work also suggested that full-length snRNAseq of whole human SAT facilitated the identification of distinct adipocyte subpopulations whilst scRNAseq using isolated adipocytes from the same sample failed to identify any distinct subpopulations.…”

Section: The Existence Of White Adipocyte Subpopulationsmentioning

confidence: 99%

“…This work also suggested that full-length snRNAseq of whole human SAT facilitated the identification of distinct adipocyte subpopulations whilst scRNAseq using isolated adipocytes from the same sample failed to identify any distinct subpopulations. This highlighted that the latter approach may not be appropriate for the identification of adipocyte subtypes [ 16 ▪ ]. It is also important to consider that studies using snRNAseq are marked with inherent transcript enrichment and detection bias as nuclear transcripts do not represent the total transcriptome of a single cell.…”

Section: The Existence Of White Adipocyte Subpopulationsmentioning

confidence: 99%

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Heterogeneity of white adipocytes in metabolic disease

Bilson

Sethi

Byrne

2023

Current Opinion in Clinical Nutrition &Amp; Metabolic Care

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Purpose of review This review aims to discuss the most recent evidence identifying the presence of distinct white adipocyte subpopulations in white adipose tissue (WAT) and how these may be altered with increasing adiposity and/or metabolic disease. We conceptualize how changes in adipocyte subpopulations may contribute to alterations in WAT function and the development of metabolic diseases such as type 2 diabetes mellitus (T2DM), nonalcoholic fatty liver disease (NAFLD) and cardiovascular disease (CVD). Recent findings Studies utilizing novel analytical approaches support the existence of distinct white adipocyte subpopulations in both human and murine WAT. Adipocyte subtypes are potentially functionally distinct and may have different roles in WAT function and obesity-associated metabolic diseases. Summary The exploration of white adipocyte heterogeneity using novel analytical technologies, has unveiled a new layer of complexity in the study of WAT biology. Interrogation of potential functional differences between adipocyte subpopulations and their role in the function of different WAT depots is now needed. Through understanding the mechanisms regulating white adipocyte subtype development and potential pathophysiological consequences of changes in the presence of adipocyte subpopulations, studies could provide novel therapeutic targets for the treatment of T2DM, NAFLD, and CVD.

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Section: The Existence Of White Adipocyte Subpopulationsmentioning

confidence: 99%

Section: The Existence Of White Adipocyte Subpopulationsmentioning

confidence: 99%

Section: The Existence Of White Adipocyte Subpopulationsmentioning

confidence: 99%

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Heterogeneity of white adipocytes in metabolic disease

Bilson

Sethi

Byrne

2023

Current Opinion in Clinical Nutrition &Amp; Metabolic Care

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“…For more details, refer to Whytock et al., (2022). 1 Quality control was performed with R package scran. 3 Intergration and clustering were performed with R package Seurat.…”

Section: Expected Outcomesmentioning

confidence: 99%

“…Figure reprinted with permission from Whytock et al., 2022. 1 (B) Pseudotime analysis showing adipocyte differentiation in vivo using snRNA-Seq. (C) RNA velocity analysis of pre-adipocyte and adipocyte subpopulations using snRNA-Seq.…”

Section: Expected Outcomesmentioning

confidence: 99%

Isolation of nuclei from frozen human subcutaneous adipose tissue for full-length single-nuclei transcriptional profiling

et al. 2023

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Mitochondrial Function and Dysfunction in White Adipocytes and Therapeutic Implications

Wang,

Huynh,

2024

Comprehensive Physiology

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For a long time, white adipocytes were thought to function as lipid storages due to the sizeable unilocular lipid droplet that occupies most of their space. However, recent discoveries have highlighted the critical role of white adipocytes in maintaining energy homeostasis and contributing to obesity and related metabolic diseases. These physiological and pathological functions depend heavily on the mitochondria that reside in white adipocytes. This article aims to provide an up‐to‐date overview of the recent research on the function and dysfunction of white adipocyte mitochondria. After briefly summarizing the fundamental aspects of mitochondrial biology, the article describes the protective role of functional mitochondria in white adipocyte and white adipose tissue health and various roles of dysfunctional mitochondria in unhealthy white adipocytes and obesity. Finally, the article emphasizes the importance of enhancing mitochondrial quantity and quality as a therapeutic avenue to correct mitochondrial dysfunction, promote white adipocyte browning, and ultimately improve obesity and its associated metabolic diseases. © 2024 American Physiological Society. Compr Physiol 14:5581‐5640, 2024.

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Single cell full-length transcriptome of human subcutaneous adipose tissue reveals unique and heterogeneous cell populations

Cited by 29 publications

References 64 publications

Heterogeneity of white adipocytes in metabolic disease

Heterogeneity of white adipocytes in metabolic disease

Isolation of nuclei from frozen human subcutaneous adipose tissue for full-length single-nuclei transcriptional profiling

Mitochondrial Function and Dysfunction in White Adipocytes and Therapeutic Implications

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