Single cell gel electrophoresis assay: methodology and applications

Rojas, Emilio; López, Marcos Valdivia; Valverde, Mahara

doi:10.1016/s0378-4347(98)00313-2

Cited by 472 publications

(273 citation statements)

References 240 publications

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“…The alkaline comet assay is increasingly used in industrial in vitro genotoxicity testing (Rojas et al, 1999;Hartmann et al, 2001) and has also been used an important tool to evaluate the genotoxic potential of compounds in vivo (Rojas et al, 1999;Sekihashi et al, 2002). Our comet assay results for peripheral blood leukocytes from blood taken from 12-day old female and male Swiss mice 4 h and 24 h after treatment are summarized in Table 1.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae)

2008

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The northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) is very rich in anthocyanins, natural pigments which have strong antioxidant properties and potential health benefits, resulting in the worldwide use the blueberry as a medicinal plant. We investigated the mutagenic potential of simple hydroalcoholic extracts of V. corymbosum acutely administrated by gavage to Swiss mice at doses of 1 g kg -1 , 1.5 g kg -1 and 2 g kg -1 . Peripheral blood cells were collected 4 h and 24 h post-gavage and assessed by the alkaline comet assay, with further blood samples being collected at 48 h and 72 h for assessment using the micronucleus (MN) assay. Our results show that the V. corymbosum extracts did not induce any statistically significant increase in the average amount of DNA damage in peripheral blood leukocytes. However, we did record a significant increase in the frequency of micronucleated polychromatic erythrocytes at the three doses tested.

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Section: Resultsmentioning

confidence: 99%

Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae)

2008

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“…Para realização do Teste Cometa foi utilizado como amostra biológica o sangue periférico. A metodologia utilizada foi modificada a partir de procedimentos previamente descritos por Rojas et al 23 , Pitozzi et al 24 , Hartman et al 25 , de Tice et al 26 e Azqueta et al 27 . O uso de controles na realização desse procedimento analí-tico permite verificar se o teste foi satisfatório, pois muitas substâncias podem provocar danos ao DNA e, assim, o teste pode funcionar como controle positivo.…”

Section: Análise Do Teste Cometa Em Células Sanguíneasunclassified

Avaliação da exposição ocupacional ao benzeno em trabalhadores frentistas e analistas de combustíveis utilizando o Teste Cometa como biomarcador de genotoxicidade

Campos

Fernandes

André

2017

Rev. bras. saúde ocup.

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Resumo Introdução: frentistas e analistas de combustíveis estão expostos a vários compostos orgânicos voláteis presentes na gasolina, incluindo benzeno, que se destaca por sua importância toxicológica. Objetivo: avaliar a exposição ocupacional ao benzeno na gasolina, utilizando o Teste Cometa como biomarcador de genotoxicidade em comparação ao ácido trans,trans-mucônico urinário (AttM) como biomarcador de exposição ao benzeno. Métodos: estudo de corte transversal com análises de biomarcadores de exposição e de genotoxicidade em grupo de expostos ocupacionalmente ao benzeno através da gasolina e grupo controle. Resultados: o Teste Cometa mostrou uma média (desvio padrão) de índice de dano, expresso em unidades arbitrárias, no grupo exposto de 28,4 (10,1) significativamente mais elevado do que no grupo não-exposto, 18,4 (10,1). O AttM urinário, em mg/g de creatinina, foi significativamente maior no grupo exposto, 1,13 (0,45), em relação ao grupo não exposto, 0,44 (0,33). Os dois biomarcadores apresentaram boa correlação linear (r=0,81; p<0,05), indicando uma forte associação entre o biomarcador de exposição e o biomarcador de efeito. Conclusão: os resultados sugerem que uma maior exposição ocupacional ao benzeno está associada a um risco aumentado de dano genotóxico entre indivíduos expostos à gasolina.

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“…The assessment of DNA stand breaks in the cells seems to be a good marker since it can give information on the genotoxic potential of the habitat (Rojas et al 1999;Dhawan et al 2009). The information is valuable since the effects of genotoxic substances (DNA damage) are detectable at a very early stage, before disadvantageous consequences of this damage appear (Jha 2008).…”

Section: Introductionmentioning

confidence: 99%

“…It enables the investigator both to detect the DNA (single and double-strand) breaks, caused by various mutagenic and toxic factors and to assess the repairing potential (Rojas et al 1999;Collins 2009;Decordier et al 2010;Collins 2014;Augustyniak et al 2016a). Such metals as Cu, Pb, Ni, Cd, Co, Zn can be found among the substances that were studied and analyzed by means of the comet assay to check whether they can generate breaks of DNA strands (Mitchelmore and Chipman 1998;Rojas et al 1999;Mourón et al 2001;Woźniak and Błasiak 2002;Augustyniak et al 2016a;Augustyniak et al 2016b).…”

Section: Introductionmentioning

confidence: 99%

Cross tolerance in beet armyworm: long-term selection by cadmium broadens tolerance to other stressors

et al. 2017

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Long lasting exposure of animals to stressing factor may lead to the selection of population able to cope with the stressor at lower cost than unexposed individuals. The aim of this study was to assess whether 130-generational selection of a beet armyworm to cadmium in food might have induced tolerance also to other stressors. The potential tolerance was assessed by means of unspecific stress markers: HSP70 concentration, DNA damage level, and energy budget indices in L5 larval instars of beet armyworm. The animals originated from Cd-exposed and control strains exposed additionally in a short-term experiment to high/low temperature or pesticide-spinosad. The application of the additional stressors caused, in general, an increase in the levels of studied parameters, in a straindependent manner. The most significant increase was found in HSP70 level in the individuals from the Cd-strain exposed to various spinosad concentration. Therefore, multigenerational contact with cadmium caused several changes that enable the insect to survive under a chronic stress, preparing the organism to the contact with an additional, new stressor. This relationship may be described as a sort of cross tolerance. This may, possibly, increase the probability of population survivorship and, at the same time, decrease the efficiency of pesticide-based plant protection efforts.

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Single cell gel electrophoresis assay: methodology and applications

Cited by 472 publications

References 240 publications

Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae)

Evaluation of the in vivo mutagenic potential of hydroalcoholic extracts of the northern highbush blueberry (Vaccinium corymbosum L. Ericales, Ericaceae) on peripheral blood cells of Swiss mice (Mus musculus Rodentia, Muridae)

Avaliação da exposição ocupacional ao benzeno em trabalhadores frentistas e analistas de combustíveis utilizando o Teste Cometa como biomarcador de genotoxicidade

Cross tolerance in beet armyworm: long-term selection by cadmium broadens tolerance to other stressors

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