Single cell gel electrophoresis on peripheral blood leukocytes of patients with oral squamous cell carcinoma

Rao, G. V.; Kumar, Gopinatha Suresh; Ahuja, Y.R.

doi:10.1111/j.1600-0714.1997.tb00234.x

Cited by 22 publications

(21 citation statements)

References 13 publications

(4 reference statements)

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“…Nevertheless, endogenous cellular processes may induce DNA damage and serve as a source of genomic instability. Previous studies have showed DNA damage in blood cells of oral cancer patients assessed by the single cel gel (comet) assay (Rao et al 1997). The results support the concept of a systemic host response in malignancy.…”

Section: Discussionsupporting

confidence: 75%

Genomic instability in blood cells is able to predict the oral cancer risk: an experimental study in rats

2008

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This study was undertaken to investigate the genomic instability on blood cells during 4-nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinogenesis by means of single cell gel (comet) and micronucleus assays. Male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12, and 20 weeks. Ten animals were used as negative control. Although no histopathological abnormalities were induced in the epithelium after 4 weeks of carcinogen exposure, genetic damage was found in blood cells as depicted by the mean tail moment and an increase of micronucleated polychromatic erythrocytes. After 12 and 20 weeks treatment, the same picture occurred, being the strong effect observed in the micronucleus induction. These periods correspond to pre-neoplastic lesions and well-differentiated squamous cell carcinomas, respectively. Taken together, our results support the idea that genomic instability on blood cells appears to be associated with the risk and progression of oral cancer, being a reliable tool for detecting early systemic conditions of malignancy.

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Section: Discussionsupporting

confidence: 75%

Genomic instability in blood cells is able to predict the oral cancer risk: an experimental study in rats

2008

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“…25 Using the same technique, the PBL of patients with OSCC, showed a significant increase in DNA damage, depending on the clinical staging and histopathological grading. 19 The DNA damage levels in PBL in patients with precancerous lesions of the cervix, showed a significant stepwise increase in the mean DNA damage in the leukocytes, from normal or control groups to patients with different grades of dysplasia.…”

Section: Discussionmentioning

confidence: 99%

“…The blood samples were then transferred in to glass bottles containing 3.8% sodium citrate. Single cell electrophoresis was carried out for both these groups and the DNA damage was quantified using a fluorescence microscope following the method outlined by Rao et al 19 and Singh et al 20 …”

Section: Collection Of Samplesmentioning

confidence: 99%

“…30 A pilot study using comet assay was done on OSCC patients. 19 This study reported increased DNA damage in peripheral blood cells of OSCC patients. …”

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confidence: 99%

“…11 In various studies, the systemic host response plays a major role in determining the findings in peripheral blood in malignancy. 19,25,27,28 As premalignant changes recognized as epithelial dysplasias and regarded as a fore runner of malignancy, a systemic host response in premalignant lesion is suggested, on the basis of the findings from this study.…”

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confidence: 99%

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Assessment of DNA Damage in Leukoplakia Patients with Different Degrees of Dysplasia

Vellappally

Binmgren

Huraib

et al. 2015

The Journal of Contemporary Dental Practice

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Background: Single cell gel electrophoresis (SCGE) assay also known as comet assay is a rapid and highly sensitive fluorescent molecular technique for detecting various forms of deoxyribonucleic acid (DNA) damage at individual cellular level. Materials and methods:The present study was done to detect the extent of DNA damage in oral leukoplakia (OL) and compare with normal individuals. The sample population was obtained from an outpatient clinic of a tertiary teaching dental institute. A total of 36 consecutive patients with leukoplakia and 10 healthy normal volunteers were recruited for the study and assessed for the extent of DNA damage using SCGE following clinical diagnosis and histological grading. Peripheral blood was obtained by venipuncture and SCGE assay was performed. Mean comet tail length was recorded and analyzed statistically to compare the extent of damage in each group. Results:The mean comet tail length seen in leukoplakia patients with moderate to severe dysplasia was 1.25 ± 0.14 mm while for the control subjects, it was 0.31 ± 0.10 mm. The difference was statistically significant (p = 0.000). On comparing within the grades of leukoplakia, a progressive trend of increasing tail length was observed with increasing grades of dysplasia. Assessment of DNA Damage in Leukoplakia Patients Conclusion:Deoxyribonucleic acid damage as measured by SCGE is seen in leukoplakia. A stepwise increase in DNA damage levels from healthy controls, through patients with non-dysplastic epithelium to varying grades of dysplasia has been observed indicating the extent of DNA damage in this high risk group.

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In vitro benzo[a]pyrene diol epoxide‐induced DNA damage and chromosomal aberrations in primary lymphocytes, smoking, and risk of squamous cell carcinoma of the head and neck

Xiong¹,

Hu²,

Li³

et al. 2007

Intl Journal of Cancer

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Cigarette smoking is a major risk factor for squamous cell carcinoma of the head and neck (SCCHN), but only a fraction of those exposed to cigarette smoke develops SCCHN, suggesting variation in individual susceptibility. Tobacco smoke contains a number of carcinogens that cause various kinds of damage to DNA. In this study, we simultaneously measured benzo [a]pyrene diol epoxideinduced DNA damage and chromosomal aberrations by the comet assay and the mutagen sensitivity assay, respectively, in cultured primary lymphocytes from newly recruited 123 patients with SCCHN and 136 age-and sex-matched controls. Using the control median as the cut-off, the elevated risk of SCCHN was 2.35 (95% CI, 1.37-4.03), 2.28 (95% CI, 1.34-3.98) and 3.25 (95% CI, 1.85-5.07) for high levels of tail extension, tail length and oliver tail moment of the comet assay, respectively, and 1.75 (95% CI, 1.04-2.94) for high levels of chromosomal aberrations of the mutagen sensitivity assay. The effects of these 2 types of measurements were additive; subjects with high levels of both DNA damage and chromosomal aberrations had a 4.77-fold increased risk (95% CI, 2.73-8.36) of SCCHN. Cigarette smoking further elevated this risk to more than 20-fold (OR 23.6; 95% CI,. These data support our previous finding that suboptimal repair contributed to susceptibility to SCCHN and the new data further suggests a possible gene-environment interaction that may play an important role in the etiology of SCCHN. Further validation studies are warranted. ' 2007 Wiley-Liss, Inc.Key words: benzo[a]pyrene diol epoxide; comet assay; cancer susceptibility; gene-environment interaction; mutagen sensitivity Squamous cell carcinoma of the head and neck (SCCHN) is the 5th common cancer in the U.S. with 40,566 new patients in 2007. 1 Cigarette smoking and alcohol consumption are the known major risk factors 2 ; however, genetic factors likely play a role in SCCHN etiology, because only a fraction of those exposed to tobacco smoke develop the disease. Benzo[a]pyrene, a classic DNA-damaging carcinogen found in tobacco smoke, is metabolized by phase I enzymes to an ultimate carcinogen, benzo[a]pyrene-7,8-diol 9,10-epoxide (BPDE), which can induce DNA damage including DNA adducts through covalent binding or oxidation, leading subsequent mutations, 3,4 if not effectively repaired. It is believed that DNA repair capacity (DRC) exist to protect the DNA from mutagenic effects of exogenous and endogenous carcinogens and that inter-individual variation in DRC may contribute to cancer susceptibility. 5-7 Rare, recessive chromosomal instability syndromes, such as xeroderma pigmentosum (XP), are characterized by various defects in DNA repair and high cancer risk. 8 In addition, the capacity to repair DNA damage also varies in the general population. 9,10 Suboptimal DRC and a high level of induced DNA damage and chromosomal aberrations have been found to be associated with enhanced susceptibility to several cancers, such as those of the lung. 4,9,11 In our previously published pilot st...

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Single cell gel electrophoresis on peripheral blood leukocytes of patients with oral squamous cell carcinoma

Cited by 22 publications

References 13 publications

Genomic instability in blood cells is able to predict the oral cancer risk: an experimental study in rats

Genomic instability in blood cells is able to predict the oral cancer risk: an experimental study in rats

Assessment of DNA Damage in Leukoplakia Patients with Different Degrees of Dysplasia

In vitro benzo[a]pyrene diol epoxide‐induced DNA damage and chromosomal aberrations in primary lymphocytes, smoking, and risk of squamous cell carcinoma of the head and neck

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