2023
DOI: 10.1101/2023.05.31.539801
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Single cell-guided prenatal derivation of primary epithelial organoids from the human amniotic and tracheal fluids

Abstract: Despite advances in prenatal diagnosis, it is still difficult to predict severity and outcomes of many congenital malformations. New patient-specific prenatal disease modelling may optimise personalised prediction. We and others have described the presence of mesenchymal stem cells in amniotic fluid (AFSC) that can generate induced pluripotent stem cells (iPSCs). The lengthy reprogramming processes, however, limits the ability to define individual phenotypes or plan prenatal treatment. Therefore, it would be a… Show more

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“…Via 3D culture in defined media, we not only preserve these progenitors, but also specifically enrich for either kidney or lung progenitors, which self-organize into tissue-specific organoids. Interestingly, a recent preprint reported derivation of organoids from AF 125 . Although these expressed kidney and lung markers, they were not analyzed at single-cell level, making it hard to determine their exact composition.…”
Section: Discussionmentioning
confidence: 99%
“…Via 3D culture in defined media, we not only preserve these progenitors, but also specifically enrich for either kidney or lung progenitors, which self-organize into tissue-specific organoids. Interestingly, a recent preprint reported derivation of organoids from AF 125 . Although these expressed kidney and lung markers, they were not analyzed at single-cell level, making it hard to determine their exact composition.…”
Section: Discussionmentioning
confidence: 99%