Single cell imaging reveals cisplatin regulating interactions between transcription (co)factors and DNA

Lin, Yu; Wu, Kui; Jia, Feifei; Chen, Ling; Wang, Zhaoying; Zhang, Yanyan; Luo, Qun; Liu, Suyan; Qi, Li; Li, Nan; Pu, Dong; Gao, Fei; Zheng, Wei; Fang, Xiaohong; Zhao, Yao; Wang, Fuyi

doi:10.1039/d0sc06760a

Cited by 18 publications

(23 citation statements)

References 50 publications

(98 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A first metal-based anticancer drug, cisplatin, mainly targets DNA [ 38 , 39 ]. Based on the success of cisplatin, much research has focused on metal complex DNA interactions [ [5] , [6] , [7] , 23 ].…”

Section: Resultsmentioning

confidence: 99%

Multi-target activity of copper complexes: Antibacterial, DNA binding, and molecular docking with SARS-CoV-2 receptor

Arthi

Mahendiran

Kaya

et al. 2023

Chemico-Biological Interactions

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Multi-target activity of copper complexes: Antibacterial, DNA binding, and molecular docking with SARS-CoV-2 receptor

Arthi

Mahendiran

Kaya

et al. 2023

Chemico-Biological Interactions

View full text Add to dashboard Cite

“…Secondary‐ion mass spectrometry (SIMS), including NanoSIMS and ToF‐SIMS, is a high‐resolution surface analysis technique recently applied for imaging botanical, microbiological, and biomedical samples at single cell level [53–56] . However, SIMS imaging of specific biomacromolecules in single cells is still highly challenging due to the lack of characteristic fragment or molecular ions.…”

Section: Resultsmentioning

confidence: 99%

“…Secondary-ion mass spectrometry (SIMS), including NanoSIMS and ToF-SIMS, is a high-resolution surface analysis technique recently applied for imaging botanical, microbiological, and biomedical samples at single cell level. [53][54][55][56] However, SIMS imaging of specific biomacromolecules in single cells is still highly challenging due to the lack of characteristic fragment or molecular ions. Ln elements had been conjugated with antibodies for Nano-SIMS imaging of multiple biomarkers in tumor sections benefited from their wide selection of elements or isotopes and low natural abundance in human body.…”

Section: Forschungsartikelmentioning

confidence: 99%

Bioorthogonal Lanthanide Molecular Probes for Near‐Infrared Fluorescence and Mass Spectrometry Imaging

et al. 2022

Self Cite

View full text Add to dashboard Cite

We report here the development of clickable and highly near‐infrared (NIR) fluorescent lanthanide (Ln) complexes for bioorthogonal labeling of biomolecules. These azide‐ or alkyne‐functionalized Ln complexes are hydrophilic and fluorogenic, exhibiting a strong increase of NIR fluorescence upon conjugation with biomolecules. Metabolic labeling of biomolecules with azide or alkyne, followed by click labeling with the Ln complexes, enables NIR fluorescence (NIRF) imaging of DNA, RNA, proteins, and glycans in cells. Furthermore, multicolor imaging is performed by combining click‐labeling with the Ln complexes and immunostaining. In addition, the Ln complexes is compatible with click‐expansion microscopy (click‐ExM), which enables high‐resolution NIRF imaging of cellular glycoproteins. Finally, the Ln complexes can be used for time‐of‐flight secondary‐ion mass spectrometry (ToF‐SIMS) imaging, thus achieving the first example of dual‐modal imaging combining NIRF and SIMS microscopies.

show abstract

“…With a spatial resolution of 200 -300 nm, ToF-SIMS image is able to visualize the subcellular distribution of chemicals. 26,[35][36][37][38][39][40] However, the low content of trace elements and complex components in cells made it difficult to obtain high quality of images which can discriminate the trace element located in cytoplasm and nuclei of the single cells as shown in Fig. 4 and Fig.…”

Section: Resultsmentioning

confidence: 99%

The Disturbance of Anticancer Drug Cisplatin to Cellular Homeostasis of Trace Elements Revealed by ICP-MS and ToF-SIMS

Jia¹,

Li²,

Zhang³

et al. 2021

At.Spectrosc.

Self Cite

View full text Add to dashboard Cite

Trace elements play important roles in many physiological processes. The disorder in the metabolism and/or homeostasis of the trace elements will cause pathogenic changes, and finally lead to the development of diseases. This present work aimed to investigate the effect of anticancer metallodrug cisplatin on the homeostasis of trace metal elements, iron (Fe), zinc (Zn) and copper (Cu), in both human HEK293 normal cells and A549 lung cancer cells by using inductively coupled plasma mass spectrometry (ICP-MS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). The levels of trace metal elements in both whole cells and nuclei subjected to cisplatin treatment were measured by ICP-MS, and results showed that accompanying with the increasing concentration of cisplatin, the content of Fe inside whole cells and nuclei of both normal and cancer cells increased, implying that cisplatin may induce ferroptosis, contributing to its anticancer activity. While the level of Cu and Zn in the whole cells increased with increase in cisplatin concentration, the level of Cu and Zn, in particular the latter, in nuclei decreased with increase of cisplatin concentration. These implicate that cisplatin may compete with Zn for binding to metalloproteins in nuclei, leading to efflux of Zn from nucleus. The normalized signal intensity of Fe, Zn, and Cu in single cells detected by ToF-SIMS increased accompanying with increased cisplatin concentration, being in line with that obtained by quantitative ICP-MS on large quantity of cells. These findings provide novel insights into better understanding in the mechanism of action of cisplatin.Trace metal elements, such as iron, zinc, copper, are essential in the human body as they consist of many metalloproteins or enzymes which are involved in vital physiological regulation processes and play crucial roles in maintaining the normal function

show abstract

Single cell imaging reveals cisplatin regulating interactions between transcription (co)factors and DNA

Abstract: Cisplatin is an extremely successful anticancer drug, and is commonly thought to target DNA. However, the way in which cisplatin-induced DNA lesions regulate interactions between transcription factors/cofactors and genomic DNA...

Cited by 18 publications

References 50 publications

Multi-target activity of copper complexes: Antibacterial, DNA binding, and molecular docking with SARS-CoV-2 receptor

Multi-target activity of copper complexes: Antibacterial, DNA binding, and molecular docking with SARS-CoV-2 receptor

Bioorthogonal Lanthanide Molecular Probes for Near‐Infrared Fluorescence and Mass Spectrometry Imaging

The Disturbance of Anticancer Drug Cisplatin to Cellular Homeostasis of Trace Elements Revealed by ICP-MS and ToF-SIMS

Contact Info

Product

Resources

About