Single-cell isolation using a DVD optical pickup

Kasukurti, A.; Potcoava, Mariana; Desai, Sanjay A.; Eggleton, Charles D.; Marr, David W. M.

doi:10.1364/oe.19.010377

Cited by 24 publications

(22 citation statements)

References 33 publications

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“…For the lower optical powers required for manipulating polystyrene microparticles we use an inexpensive and compact off-the-shelf DVD optical pickup unit 26 . With the added benefit of reduced cost and small footprint, this unit includes a 500 mW 660 nm laser diode that is focused into a 2 µm spot (70 mW at sample plane due to losses in the optical path) using the integrated optics (NA ~ 0.6) (Figure 4) to generate up to 15 pN of optical trapping force 26 . We also employ the electromagnetically suspended movable focusing lens of the optical pickup unit driven by an inexpensive programmable microcontroller (Arduino UNO).…”

Section: Methodsmentioning

confidence: 99%

“…We demonstrate function with red blood cells (RBCs) isolated with a bench-top optical trap onto microscope slides. We also demonstrate an inexpensive and compact implementation of the same with 4.18 µm fluorescent polystyrene microparticles isolated with an off-the-shelf DVD optical pickup generated optical trap 26 . With no need for external pumps or active components this is an inexpensive and easy to operate system.…”

Section: Introductionmentioning

confidence: 99%

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A simple microfluidic dispenser for single-microparticle and cell samples

et al. 2014

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Non-destructive isolation of single-cells has become an important need for many biology research laboratories; however, there is a lack of easily employed and inexpensive tools. Here, we present a single-particle sample delivery approach fabricated from simple, economical components that may address this need. In this, we employ unique flow and timing strategies to bridge the significant force and length scale differences inherent in transitioning from single particle isolation to delivery. Demonstrating this approach, we use an optical trap to isolate individual microparticles and red blood cells that are dispensed within separate 50 µl droplets off a microfluidic chip for collection into microscope slides or microtiter plates.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A simple microfluidic dispenser for single-microparticle and cell samples

et al. 2014

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“…The Michelson interferometer required for the MR-OCT can easily be integrated into a PUH. Considering the availability of such PUHs for a purchase price down to about 10 USD, it can be envisioned to build the opto-mechanical core system for a MR-OCT system at a very competitive price [23]. All other required components are widely available and can be used to build a MR-OCT system with low acquisition costs.…”

Section: Introductionmentioning

confidence: 99%

Dermascope guided multiple reference optical coherence tomography

Dsouza¹,

Subhash²,

Neuhaus³

et al. 2014

Biomed. Opt. Express

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Abstract:In this paper, we report the feasibility of integrating a novel low cost optical coherence tomography (OCT) system with a dermascope for point-of-care applications. The proposed OCT system is based on an enhanced time-domain optical coherence tomographic system, called multiple reference OCT (MR-OCT), which uses a single miniature voice coil actuator and a partial mirror for extending the axial scan range. The system can simultaneously register both the superficial dermascope image and the depth-resolved OCT sub-surface information by an interactive beam steering method. A practitioner is able to obtain the depth resolved information of the point of interest by simply using the mouse cursor. The proposed approach of combining a dermascope with a low cost OCT provides a unique powerful optical imaging modality for a range of dermatological applications. Hand-held dermascopic OCT devices would also enable point of care and remote health monitoring.

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“…In the past, the OPHs have been used to detect the linear and angular displacements, to be an optical accelerometer, and to detect the profile of a surface [1]- [3]. They were also used as optical tweezer and light source of the laser direct WRITE system [4]- [6]. There were a few studies to use the OPH to be an optical microscope (OM).…”

Section: Introductionmentioning

confidence: 99%

A Compact and Low-Cost Optical Pickup Head-Based Optical Microscope

Lee

Chao

2014

IEEE Trans. Magn.

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An optical pickup head (OPH) is a compact and low-cost but of high-precision optical system. In the past, it has been used for much scientific research besides optical disc storage, in particular, for the length-related measurements. In this paper, we propose a compact and low-cost OPH-based optical microscope (OM). A CMOS sensor disassembled from the web camera is fixed below the fold mirror of the OPH. The revised structure is simple and low cost but without permanently altering the original structure of the OPH. The user can choose operation modes of either the OPH or OM by a simple switching. We estimated that the optical magnification of the OM is 15.5X. The experimentally measured resolution of the OM is ∼2 µm for the visible light, and it achieved diffraction limit for narrow-band light source. From the results of optical simulation, the main factor deteriorating the resolution is chromatic aberration. Astigmatic aberration is not important. Some plant cells investigation results using the microscope are also demonstrated in this paper.Index Terms-Chromatic aberration, optical microscope (OM), optical pickup head (OPH), plant cell, resolution.

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Single-cell isolation using a DVD optical pickup

Cited by 24 publications

References 33 publications

A simple microfluidic dispenser for single-microparticle and cell samples

A simple microfluidic dispenser for single-microparticle and cell samples

Dermascope guided multiple reference optical coherence tomography

A Compact and Low-Cost Optical Pickup Head-Based Optical Microscope

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