Single Cell Isolation Using Optical Tweezers

Keloth, Anusha; Anderson, Owen A.; Risbridger, Donald R.; Paterson, Lynn

doi:10.20944/preprints201806.0371.v1

Cited by 42 publications

(41 citation statements)

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“…The proposed NOTOD is not only compact, having high optical efficiency, but also a flexible tool for optical method to control trapped bead in space and to stretch different kind of DNA molecules. Using organic dye with high nonlinearity, the optical trap efficiency of NOTOD is high enough, which can trap nanoparticle [21,22] and stretch DNA molecule [44][45][46][47], instead of using ultrafast pulsed laser.…”

Section: Discussionmentioning

confidence: 99%

Nonlinear Optical Tweezers As an Optical Method for Controlling Particles with High Trap Efficiency

Quy¹

2019

Comm. in Phys.

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Optical tweezers have seen as an essential tool for the manipulation dielectric microparticles and nanoparticles due to its non-contact action and high resolution of optical force. Up to now, there has been a lot of optical tweezers applications in the fields of biophysics, chemistry, medical science and nanoscience. Recently, optical tweezers have been theoretically and experimentally developing for the nanomechanical characterization of various kinds of biological cells. The configuration of optical tweezers has been day after day improving to enhance the trapping efficiency, spatial and temporal resolution and easy to control trapped objects. In common trend of optical tweezers improvements, we will discuss in detail of the several configurations of nonlinear optical tweezers using nonlinear materials as the added lens. We will also address the advantages of nonlinear optical tweezers, such as enhance optical efficiency, reduce trapping region, simplify controlling all-optical method. Finally, we present discussions about the specific properties of nonlinear optical tweezers used for stretch DNA molecule as example and an ideal to improve nonlinear optical tweezers using thin layer of organic dye proposed for going time.

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Section: Discussionmentioning

confidence: 99%

Nonlinear Optical Tweezers As an Optical Method for Controlling Particles with High Trap Efficiency

Quy¹

2019

Comm. in Phys.

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“…However, it can as yet not be used as the only technique for any kind of diagnostics, and is either combined with other tests to create assays [63,66], or needs massive automation and robotics [156] or artificial intelligence to arrive at a diagnosis [156,157]. The usage of optical traps for single cell diagnosis is mostly limited to optical stretching [158], cell-sorting [66,154,[159][160][161][162], and measuring of refractive indices [140,[162][163][164]. Especially in combination with automatization, optical traps for single cell diagnostics have a great potential, but currently it is among the more expensive and less-performing technologies.…”

Section: Figurementioning

confidence: 99%

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

Hochstetter

2020

Micromachines

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In the last three decades, microfluidics and its applications have been on an exponential rise, including approaches to isolate rare cells and diagnose diseases on the single-cell level. The techniques mentioned herein have already had significant impacts in our lives, from in-the-field diagnosis of disease and parasitic infections, through home fertility tests, to uncovering the interactions between SARS-CoV-2 and their host cells. This review gives an overview of the field in general and the most notable developments of the last five years, in three parts: 1. What can we detect? 2. Which detection technologies are used in which setting? 3. How do these techniques work? Finally, this review discusses potentials, shortfalls, and an outlook on future developments, especially in respect to the funding landscape and the field-application of these chips.

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“…3(B). In brief, microfluidic enrichment systems have been developed making use of acoustophoresis, 192 electrophoresis, 186 dielectrophoresis, 193 magnetophoresis, 194 or optical forces, 195 as well as passive systems that use inertial effects, 196 filters, 197 or immobilization processes. 198 While demonstrating significant potential, many of the separation systems explored in the literature are in the prototype or proof-of-concept stage, 189 with few demonstrating the performance required for fully integrated systems.…”

Section: Cell Enrichment Strategiesmentioning

confidence: 99%

A Review of Design Considerations for Hemocompatibility within Microfluidic Systems

Szydzik

Brazilek

Nesbitt

2020

Semin Thromb Hemost

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The manipulation of blood within in vitro environments presents a persistent challenge, due to the highly reactive nature of blood, and its multifaceted response to material contact, changes in environmental conditions, and stimulation during handling. Microfluidic Lab-on-Chip systems offer the promise of robust point-of-care diagnostic tools and sophisticated research platforms. The capacity for precise control of environmental and experimental conditions afforded by microfluidic technologies presents unique opportunities that are particularly relevant to research and clinical applications requiring the controlled manipulation of blood. A critical bottleneck impeding the translation of existing Lab-on-Chip technology from laboratory bench to the clinic is the ability to reliably handle relatively small blood samples without negatively impacting blood composition or function. This review explores design considerations critical to the development of microfluidic systems intended for use with whole blood from an engineering perspective. Material hemocompatibility is briefly explored, encompassing common microfluidic device materials, as well as surface modification strategies intended to improve hemocompatibility. Operational hemocompatibility, including shear-induced effects, temperature dependence, and gas interactions are explored, microfluidic sample preparation methodologies are introduced, as well as current techniques for on-chip manipulation of the whole blood. Finally, methods of assessing hemocompatibility are briefly introduced, with an emphasis on primary hemostasis and platelet function.

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Single Cell Isolation Using Optical Tweezers

Abstract: Optical tweezers offer a non-contact method for selecting single cells and translocating

Cited by 42 publications

References 0 publications

Nonlinear Optical Tweezers As an Optical Method for Controlling Particles with High Trap Efficiency

Nonlinear Optical Tweezers As an Optical Method for Controlling Particles with High Trap Efficiency

Lab-on-a-Chip Technologies for the Single Cell Level: Separation, Analysis, and Diagnostics

A Review of Design Considerations for Hemocompatibility within Microfluidic Systems

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