Single cell mechanics of keratinocyte cells

Lulevich, Valentin; Yang, Hsin-Ya; Isseroff, Roslyn Rivkah; Liu, Gang-yu

doi:10.1016/j.ultramic.2010.07.009

Cited by 82 publications

(87 citation statements)

References 40 publications

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“…Using atomic force microscopy (AFM)-based single-cell compression to examine resistance against external pressure and global rupturing forces, keratinocytes were 6 -70 times stiffer than most other cell types. Selective disruption of actin filaments and microtubules did not significantly alter keratinocyte mechanics (Lulevich et al 2010). However, application of AFM to keratin-deficient individual keratinocytes revealed a significant softening of keratin-deficient cells (Ramms et al 2013).…”

Section: Keratins As Main Determinants For the Mechanical Integrity Omentioning

confidence: 99%

Desmosomes and Intermediate Filaments: Their Consequences for Tissue Mechanics

Hatzfeld

Keil

Magin

2017

Cold Spring Harb Perspect Biol

116

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Adherens junctions (AJs) and desmosomes connect the actin and keratin filament networks of adjacent cells into a mechanical unit. Whereas AJs function in mechanosensing and in transducing mechanical forces between the plasma membrane and the actomyosin cytoskeleton, desmosomes and intermediate filaments (IFs) provide mechanical stability required to maintain tissue architecture and integrity when the tissues are exposed to mechanical stress. Desmosomes are essential for stable intercellular cohesion, whereas keratins determine cell mechanics but are not involved in generating tension. Here, we summarize the current knowledge of the role of IFs and desmosomes in tissue mechanics and discuss whether the desmosome-keratin scaffold might be actively involved in mechanosensing and in the conversion of chemical signals into mechanical strength.

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Section: Keratins As Main Determinants For the Mechanical Integrity Omentioning

confidence: 99%

Desmosomes and Intermediate Filaments: Their Consequences for Tissue Mechanics

Hatzfeld

Keil

Magin

2017

Cold Spring Harb Perspect Biol

116

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“…Unstructured, diffuse distribution of actin in the cytoplasm is typical for non-confluent keratinocytes. [47,48]. Concentration of actin on the cell periphery and at cell-cell junctions reveal that PCL and PCL/collagen nanofibrous scaffolds support cellular interactions among keratinocytes and direct them for the formation of strong cell sheets required for wound healing and epidermal tissue engineering [50,51].…”

Section: Discussionmentioning

confidence: 99%

Electrospinning of nanofibrous polycaprolactone (PCL) and collagen-blended polycaprolactone for wound dressing and tissue engineering

Zeybek¹,

Duman²,

Ürkmez³

2014

uujms

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Fabrication of nanofibrous biomaterials based on natural materials through various techniques is a popular research topic, particularly for biomedical applications. Electrospinning, a well-established technique for nanofiber production has also been extended for producing nanofibrous structures of natural materials that mimic natural extracellular matrix of mammalian tissues. Collagen nanofiber production utilizes hexafluoro propanol (HFP) as a solvent for electrospinning. A novel cost-effective electrospun nanofibrous membrane is established for wound dressing and allogeneic cultured epidermal substitute through the cultivation of human dermal keratinocytes for skin defects. Several synthetic polymers such as polycaprolactone (PCL) are generally electrospun for tissue engineering applications because of their remarkable mechanical stability and slow degradation rates. The large surface area of the polymer nanofibers with specific modifications facilitates cell adhesion and control of their cellular functions. The objectives of this study were to optimize fabrication parameters of electrospun nanofibrous membranes from biodegradable PCL and collagen-blended nanofibrous membranes to combine mechanical integrity and spinnability of PCL with high biocompatibility of collagen, and to examine keratinocyte attachment, morphology, proliferation, and cell-matrix interactions. Results prove that the porous nanofibrous PCL and modified PCL-blended collagen nanofibrous membranes are suitable for the attachment and proliferation of keratinocytes, and might have the potential to be applied as wound dressing as well as in tissue engineering as an epidermal substitute for the treatment of skin defects and burn wounds.

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“…Oberleithner et al [37] investigated the influence of potassium on the stiffness of endothelium cells and found that the increase in potassium can soften the endothelial cells. Lulevich et al [60] found that keratinocytes were 6-70 times stiffer than other cell types (e.g., white blood cell, breast epithelial, fibroblast, or neuronal cells) using 40-lm-diameter sphere tip. Nikkhah [39] found that the growth medium composition can influence the elastic properties of cells.…”

Section: Principle and Methods Of Afm Indentation Techniquesmentioning

confidence: 99%

Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy

Liu

et al. 2014

Chin. Sci. Bull.

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The advent of atomic force microscopy (AFM) provides a powerful tool for investigating the behaviors of single living cells under near physiological conditions. Besides acquiring the images of cellular ultra-microstructures with nanometer resolution, the most remarkable advances are achieved on the use of AFM indenting technique to quantify the mechanical properties of single living cells. By indenting single living cells with AFM tip, we can obtain the mechanical properties of cells and monitor their dynamic changes during the biological processes (e.g., after the stimulation of drugs). AFM indentation-based mechanical analysis of single cells provides a novel approach to characterize the behaviors of cells from the perspective of biomechanics, considerably complementing the traditional biological experimental methods. Now, AFM indentation technique has been widely used in the life sciences, yielding a large amount of novel information that is meaningful to our understanding of the underlying mechanisms that govern the cellular biological functions. Here, based on the authors' own researches on AFM measurement of cellular mechanical properties, the principle and method of AFM indentation technique was presented, the recent progress of measuring the cellular mechanical properties using AFM was summarized, and the challenges of AFM single-cell nanomechanical analysis were discussed.

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Single cell mechanics of keratinocyte cells

Cited by 82 publications

References 40 publications

Desmosomes and Intermediate Filaments: Their Consequences for Tissue Mechanics

Desmosomes and Intermediate Filaments: Their Consequences for Tissue Mechanics

Electrospinning of nanofibrous polycaprolactone (PCL) and collagen-blended polycaprolactone for wound dressing and tissue engineering

Research progress in quantifying the mechanical properties of single living cells using atomic force microscopy

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