Single-Cell Metabolomics: Analytical and Biological Perspectives

Zenobi, Renato

doi:10.1126/science.1243259

Cited by 578 publications

(503 citation statements)

References 90 publications

Supporting

Mentioning

488

Contrasting

Unclassified

Order By: Relevance

“…Another interesting topic is the analysis of metabolic fluxes, also called fluxomics (Mo et al 2009;Celton et al 2012). In budding yeast, the first single-cell metabolomic studies have recently appeared (Ibanez et al 2013;Zenobi 2013), and methods for highthroughput screening of low-volume cultures from 96-well plates are also being developed (Ewald et al 2009 …”

Section: Discussionmentioning

confidence: 99%

Metabolomic Analysis of Schizosaccharomyces pombe: Sample Preparation, Detection, and Data Interpretation

Pluskal

Yanagida

2016

Cold Spring Harb Protoc

View full text Add to dashboard Cite

Metabolomics is a modern field of chemical biology that strives to simultaneously quantify hundreds of cellular metabolites. Techniques for metabolomic analysis in Schizosaccharomyces pombe have only recently been developed. Here we introduce methods that provide a complete workflow for metabolomic analysis in S. pombe. Based on available literature, we estimate the yeast metabolome to comprise on the order of several thousand different metabolites. We discuss the feasibility of extraction and detection of such a large number of metabolites, and the influences of various parameters on the results. Among the parameters addressed are cell cultivation conditions, metabolite extraction techniques, and detection and quantification methods. Further, we provide recommendations on data management and data processing for metabolomic experiments, and describe possible pitfalls regarding the interpretation of metabolomic data. Finally, we briefly discuss potential future developments of this technique.

show abstract

Section: Discussionmentioning

confidence: 99%

Metabolomic Analysis of Schizosaccharomyces pombe: Sample Preparation, Detection, and Data Interpretation

Pluskal

Yanagida

2016

Cold Spring Harb Protoc

View full text Add to dashboard Cite

show abstract

“…There are four major methods used to quantify the metabolome of single cells: Fluorescence-Based Detection, Fluorescence Biosensors, Fluorescence resonance energy transfer (FRET) based-biosensors and Mass Spectroscopy. The first three methods listed use fluorescence microscopy to detect molecules in a cell [9]. Usually these assays use small fluorescent tags attached to molecules of interest.…”

Section: Analytical Methodologies For Single-cell Metabolomicsmentioning

confidence: 99%

Single-Cell Metabolomics by Mass Spectrometry for Drug Discovery: Moving Forward

Raddassi¹,

Wu²,

Sf³

et al. 2017

Drug Des

View full text Add to dashboard Cite

Metabolomics is one omics approach that can be used to acquire comprehensive information on the composition of a metabolite pool to provide a functional screen of the cellular state. Single-cell metabolomics is proving useful at the molecular level for major biological research in drug development. One of the main goals of single-cell metabolomics research is to gain knowledge of cellular interaction with and in response to environmental influences on a molecular level, through a comprehensive understanding of cellular functions. In this short review, we discuss the importance of single-cell metabolomics, the concentrations of metabolites in cells, single-cell isolation, analytical methodologies for single-cell metabolomics, future prospects and a possible strategy for studying single-cell metabolomics by combining Fluorescence-activated cell sorting (FACS), Cytometry by time-of-flight (CyTOF) and Liquid Chromatography with tandem mass spectrometry (LC-MS/MS).

show abstract

“…At this point, we want to emphasize the many comprehensive review articles that discuss the challenges and current approaches of single cell analysis (e.g., Zenobi [5] Fritzsch et al [6], Schmid et al [7], Borland et al [8]). Furthermore, in a recent issue of ABC (406/14) entitled "Multiplex platforms in diagnostics and bioanalytics," the state of the art of those methods is discussed, which are already fit for purpose of measuring at cellular levels.…”

mentioning

confidence: 99%