Single-Cell Proteomics: Technology and Applications

Prasad, Minakshi; Kumar, Rajesh; Ghosh, Mayukh; Saini, Hari Mohan; Ranjan, Koushlesh; Brar, Basanti; Prasad, Gaya

doi:10.1016/b978-0-12-814919-5.00014-2

Cited by 20 publications

(22 citation statements)

References 67 publications

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“…The most common hurdle in SCM analyses is the isolation of single target cell followed by its careful handling and delivery to the downstream analytical platforms because it is directly reflected over the precision of SCM data. Limiting dilutions, manual cell-picking by micromanipulator and laser micro dissection are the common manual methods having relatively low throughput yet applied for single cell analyses where as fluorescence-based cell sorting, high-density microarrays and microfluidics are the most popular automatic high throughput single-cell isolation techniques employed for SCM (Gross et al, 2015;Minakshi et al, 2019b; Figure 1 and Table 2). High cost involvement is a major limitation associated with all of these high end techniques.…”

Section: Sample Preparation Methods For Single Cell Isolation For Scmmentioning

confidence: 99%

Single Cell Metabolomics: A Future Tool to Unmask Cellular Heterogeneity and Virus-Host Interaction in Context of Emerging Viral Diseases

et al. 2020

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Section: Sample Preparation Methods For Single Cell Isolation For Scmmentioning

confidence: 99%

Single Cell Metabolomics: A Future Tool to Unmask Cellular Heterogeneity and Virus-Host Interaction in Context of Emerging Viral Diseases

et al. 2020

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“…Single cell proteomics is basically the snapshot of the protein landscape of a single cell for a given time [ 134 ]. Single cell omics is a powerful depiction of tumor heterogeneity, among which scProteomics data is the most reliable one, as it shows the actual translational status of each and every cell.…”

Section: Future Prospect: Aptamers In Stem Cell Research Single Cell Proteomics and Immunotherapymentioning

confidence: 99%

“…However, available technologies (Flow cytometry, Mass cytometry, etc.) do have several pitfalls, like poor sensitivity, inefficient sampling procedure, reduced multiplexing capacity, etc., [ 134 ]. The analysis of a maximum of 50–100 proteins in a single go has been achieved to date, which is actually a very minute part of the entire proteome [ 135 ].…”

Section: Future Prospect: Aptamers In Stem Cell Research Single Cell Proteomics and Immunotherapymentioning

confidence: 99%

Identification and Engineering of Aptamers for Theranostic Application in Human Health and Disorders

Basu

Chakraborty

Pal

et al. 2021

IJMS

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An aptamer is a short sequence of synthetic oligonucleotides which bind to their cognate target, specifically while maintaining similar or higher sensitivity compared to an antibody. The in-vitro selection of an aptamer, applying a conjoining approach of chemistry and molecular biology, is referred as Systematic Evolution of Ligands by Exponential enrichment (SELEX). These initial products of SELEX are further modified chemically in an attempt to make them stable in biofluid, avoiding nuclease digestion and renal clearance. While the modification is incorporated, enough care should be taken to maintain its sensitivity and specificity. These modifications and several improvisations have widened the window frame of aptamer applications that are currently not only restricted to in-vitro systems, but have also been used in molecular imaging for disease pathology and treatment. In the food industry, it has been used as sensor for detection of different diseases and fungal infections. In this review, we have discussed a brief history of its journey, along with applications where its role as a therapeutic plus diagnostic (theranostic) tool has been demonstrated. We have also highlighted the potential aptamer-mediated strategies for molecular targeting of COVID-19. Finally, the review focused on its future prospective in immunotherapy, as well as in identification of novel biomarkers in stem cells and also in single cell proteomics (scProteomics) to study intra or inter-tumor heterogeneity at the protein level. Small size, chemical synthesis, low batch variation, cost effectiveness, long shelf life and low immunogenicity provide advantages to the aptamer over the antibody. These physical and chemical properties of aptamers render them as a strong biomedical tool for theranostic purposes over the existing ones. The significance of aptamers in human health was the key finding of this review.

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“…), can be used as a bait to trap caner stem cell from heterogeneous population (Figure 7A). Single cell proteomics is basically the snapshot of protein landscape of a single cell for a given time (Minakshi, 2019). Single cell omics is a powerful depiction of tumor heterogeneity at single cell level among which scProteomics data is the most reliable one as it shows the actual translational status of each and every cell.…”

Section: Therapeuticsmentioning

confidence: 99%

Identification and Engineering of Aptamers for Theranostic Application in Human Health and Disorders

Sarkar¹,

Sharma²,

Basu³

et al. 2020

Preprint

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Aptamer is short sequence of synthetic oligonucleotides which bind to their cognate target specifically while maintaining similar or higher sensitivity as antibody. Small size, chemical synthesis, low batch variation, cost effectiveness, long shelf life and above all low immunogenicity provide advantages over antibody. The in-vitro selection of aptamer applying a conjoining approach of chemistry and molecular biology is referred as Systematic Evolution of Ligands by Exponential enrichment (SELEX). These initial products of SELEX are considered as first generation aptamers, further modified chemically in an attempt to make it stable in biofluid avoiding nuclease digestion and renal clearance. These types of aptamers are called second generation aptamers. While modification is incorporated, enough care should be taken to maintain its sensitivity and specificity. These modifications and several improvisations have widened the window frame of aptamer application that is currently not only restricted to in-vitro system, but have been used in molecular imaging for disease pathology and treatment. In food industry it is used as sensor for detection of different diseases or fungal infections. In this review we have discussed a brief history of its journey, process of synthesis, different types of modifications to improve its characters. We have also focused on its applications and highlighted its role as therapeutic plus diagnostic; theranostic tools. Finally, the review is concluded with a brief discussion on future prospective in immunotherapy, as well as in identification of novel biomarkers in stem cell, and also in single cell proteomics (scProteomics) to study intra or intertumor heterogeneity at protein level.

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Single-Cell Proteomics: Technology and Applications

Cited by 20 publications

References 67 publications

Single Cell Metabolomics: A Future Tool to Unmask Cellular Heterogeneity and Virus-Host Interaction in Context of Emerging Viral Diseases

Single Cell Metabolomics: A Future Tool to Unmask Cellular Heterogeneity and Virus-Host Interaction in Context of Emerging Viral Diseases

Identification and Engineering of Aptamers for Theranostic Application in Human Health and Disorders

Identification and Engineering of Aptamers for Theranostic Application in Human Health and Disorders

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