Single‐cell RNA‐Seq reveals a highly coordinated transcriptional program in mouse germ cells during primordial follicle formation

Abstract

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Effects and potential mechanism of Ca2+/calmodulin‑dependent protein kinase II pathway inhibitor KN93 on the development of ovarian follicle

“…1). It has been previously reported that the initial stage of primordial follicle formation in mouse ovaries is embryonic 17.5 dpc (27). In the present study, CaMKII was mainly expressed in the ovarian cortex of 17.5 dpc embryos, where only a small number of primordial follicles were revealed (Fig.…”

“…Formation of primordial follicles not only determines whether there are oocytes available for fertilization, establishment and maintenance of the primordial follicle reserve pool also directly determines the reproductive life span of female animals (32,33). It has been reported that in the ovaries of mice at 17.5 days of embryonic stage, only part of the cysts rupture to form a small number of primordial follicles (27). By contrast, in the ovaries of mice after birth, a large number of cysts rupture and primordial follicles are formed continuously, before formalizing the primordial follicle pool during the first 4-5 days after birth (34).…”

Pervasive 3′-UTR Isoform Switches During Mouse Oocyte Maturation

“…We next sought to dissect the alternative RNA processing events that contributed to isoform changes between GV and MII oocytes. According to a benchmarking study ( Mehmood et al, 2020 ) and our experience ( He et al, 2021b ), we first adopted SUPPA2 ( Trincado et al, 2018 ) and rMATS ( Shen et al, 2014 ) for comparison of annotated alternative events. SUPPA2 investigated seven types of alternative events including skipped exons, retained introns, mutually exclusive exons, alternative 5′ or 3′ splice sites, and alternative first or last exons; however tandem UTRs were not included.…”

FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination

“…In an SKLRB (State Key Lab of Reproductive Medicine)independent project, many differentially expressed genes (DEGs) were screened to be the regulators of primordial follicle destiny (in chronological order, germ cells in cysts, germ cells undergoing cyst breakdown, and germ cells in follicles) in PND (post-natal-day) 0.5 ovaries through single-cell RNA sequencing, DEGs with a gradually decreasing expressing level from cyst towards follicles are thought to be more important for those in cysts or even for germ cells in meiotic prophase. 22 One of these DEGs was Fbxw24 (gene ID 382106), a gene encoding a protein with both F-box-like and WD-40 domains. The F-box domain mediates protein-protein interactions in a variety of contexts, including polyubiquitination, 23 whereas the WD-40 domain is proposed to coordinate interactions with other proteins and/or small ligands, playing a wide variety of functions in numerous eukaryotes.…”