Single-cell RNA-Seq reveals intracellular microbial diversity within immune cells during SARS-CoV-2 infection and recovery

Yadav, Sunita; Mehta, Priyanka; Soni, Jyoti; Chattopadhyay, Partha; Devi, Priti; Habyarimana, Thierry; Tardalkar, Kishore; Joshi, Meghnad; Pandey, Rajesh

doi:10.1016/j.isci.2023.108357

Cited by 4 publications

(2 citation statements)

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“…Recently, Yadav et al. reported the presence of dynamic intracellular microbial species within peripheral blood mononuclear cells (PBMCs) in healthy, infected and recovered individuals from SARS-CoV-2 using scRNA-seq ( 12 ). Furthermore, unique tools such as PathogenTrack and Yeskit have been developed to identify intracellular pathogens from scRNA-seq datasets, allowing for the assessment of transcriptomic characteristics at the individual cell level.…”

Section: Application Of Genomics To Understanding Cell-type-specific ...mentioning

confidence: 99%

“…This article emphasizes the genomics approach in understanding the interaction between host and intracellular microorganisms. These microorganisms may preferentially infect specific cell types for their survival and replication, and this preference may be influenced by factors such as functional responsibilities, availability of nutrients, immune evasion mechanisms, and replication opportunities ( 12 ). These elements collectively contribute to the microorganisms’ capacity to flourish within the host cell.…”

Section: Introductionmentioning

confidence: 99%

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Single cell genomics based insights into the impact of cell-type specific microbial internalization on disease severity

Soni,

Pandey

2024

Front. Immunol.

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Host-microbe interactions are complex and ever-changing, especially during infections, which can significantly impact human physiology in both health and disease by influencing metabolic and immune functions. Infections caused by pathogens such as bacteria, viruses, fungi, and parasites are the leading cause of global mortality. Microbes have evolved various immune evasion strategies to survive within their hosts, which presents a multifaceted challenge for detection. Intracellular microbes, in particular, target specific cell types for survival and replication and are influenced by factors such as functional roles, nutrient availability, immune evasion, and replication opportunities. Identifying intracellular microbes can be difficult because of the limitations of traditional culture-based methods. However, advancements in integrated host microbiome single-cell genomics and transcriptomics provide a promising basis for personalized treatment strategies. Understanding host-microbiota interactions at the cellular level may elucidate disease mechanisms and microbial pathogenesis, leading to targeted therapies. This article focuses on how intracellular microbes reside in specific cell types, modulating functions through persistence strategies to evade host immunity and prolong colonization. An improved understanding of the persistent intracellular microbe-induced differential disease outcomes can enhance diagnostics, therapeutics, and preventive measures.

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Section: Application Of Genomics To Understanding Cell-type-specific ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%