Single-cell RNA sequencing analysis of liver reveals the enhanced entry and release abilities of human adenovirus F41, partially explaining acute hepatitis in children

Luan, Junwen; Zhou, Yixuan; Wang, Xiao; Ming, Annan; Liu, Yuwen; Yuan, Yupei; Liang, Junhan; Zhang, Leiliang

doi:10.1016/j.jinf.2022.06.007

Cited by 4 publications

(2 citation statements)

References 11 publications

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“…The role of HAdV‐F41 in the pathogenesis of this illness in immunocompetent children has not been established yet. Some authors proposed that potential entry and release factors expressed in both fetal and adult livers may underlie the incidence of HAdV‐F41 infection 20 . Other hypotheses include an abnormal host response, possibly because of a lack of exposure to adenoviruses during the coronavirus disease 2019 (COVID‐19) pandemic, a higher susceptibility as a result of the widespread adoption of nonpharmaceutical interventions (including physical distancing, travel restrictions, masks, and regular hand washing) and subsequent reduction of exposure to various pathogens leading to increased HAdV‐F41 susceptibility.…”

Section: Discussionmentioning

confidence: 99%

Adenovirus F41 infection and liver cytolysis in adult hematopoietic stem cell transplant recipients

Lefeuvre,

Thepot,

Mercier‐Delarue

et al. 2023

Journal of Medical Virology

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Human adenoviruses (HAdVs) of the F species are commonly responsible for acute gastroenteritis. A few cases of systemic infections have been described in adults or children who have received a hematopoietic stem cell transplant (HSCT), but with no report of liver cytolysis. Since January 2022, several countries have reported an increase in cases of acute hepatitis of unknown cause in children. Adenovirus species F type 41 (HAdV‐F41) infection was predominantly identified. The objective of this study is to describe HAdV‐F41 infections diagnosed since January 2022 in adult HSCT recipients in two French hospitals. All four patients had diarrhea and liver cytolysis at the time of diagnosis of infection. HAdV viremia was observed in three patients (#1, #3, and #4), but no disseminated disease was reported. HAdV whole genome sequencing and metagenomics characterization were performed on stool and blood samples. The complete HAdV‐F41 genome sequence was obtained for three patients and phylogenetic analysis showed that the strains consisted of similar lineage (2b). We did not identify any new HAdV‐F41 strains. Metagenomics analysis found adeno‐associated virus 2 and torque‐teno virus infection in patient #1 and Epstein–Barr virus in patient #4. This is the first case series reporting liver cytolysis during HAdV‐F41 infection in adult HSCT patients.

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Section: Discussionmentioning

confidence: 99%

Adenovirus F41 infection and liver cytolysis in adult hematopoietic stem cell transplant recipients

Lefeuvre,

Thepot,

Mercier‐Delarue

et al. 2023

Journal of Medical Virology

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“…In Alabama, HAdV-F41 was detected in all five infected patients (Baker et al, 2021). A recent investigation has pointed to the synergism between HAdV-F41 and SARS-CoV-2 as responsible to activate higher levels of inflammation, which eventually can cause acute hepatitis in children (Luan et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

New primer sets to detect recent human adenovirus F41 variants in wastewater: Is it linked to the new acute hepatitis?

Pérez-Zabaleta¹,

Williams

Çetecioğlu³

2022

Preprint

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Human adenovirus type F-41 has been pursued as one of the potential reasons for the new acute hepatitis cases of unknown cause in young children. Tracking the spread of this virus in the population using wastewater-based epidemiology tools can help clinical investigations to determine its relation to this new hepatitis outbreak. In this study, methods to detect human adenovirus type F (40 and 41) and specifically type F41 were designed and implemented to quantify the amount of these pathogens in wastewater samples from Stockholm, Sweden. An assay based on reverse transcriptase quantitative polymerase chain reaction using TaqMan technology and primers targeting the three main capsid genes of adenoviruses: hexon, penton and fiber, was designed. The hexon primers were specific to adenovirus F41, while fiber primers could quantify both adenoviruses, F40 and F41. Wastewater samples from Stockholm were used to validate the designed assay and, in addition, pepper mild mottle virus (PMMoV) levels were quantified to study the data normalization. Our results can help link the occurrence of the virus variant with new cases of acute hepatitis and contribute to a better understanding of the possible causes. It can also provide valuable information that can be used in future investigations on the monitoring of human adenovirus type F in wastewater.

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Development and implementation of assays to monitor human adenovirus F40/41 in wastewater: Trends preceding, during, and following the non-A-to-E hepatitis outbreak in Stockholm

Perez-Zabaleta,

Williams,

Cetecioglu

2024

Environment International

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Single-cell RNA sequencing analysis of liver reveals the enhanced entry and release abilities of human adenovirus F41, partially explaining acute hepatitis in children

Cited by 4 publications

References 11 publications

Adenovirus F41 infection and liver cytolysis in adult hematopoietic stem cell transplant recipients

Adenovirus F41 infection and liver cytolysis in adult hematopoietic stem cell transplant recipients

New primer sets to detect recent human adenovirus F41 variants in wastewater: Is it linked to the new acute hepatitis?

Development and implementation of assays to monitor human adenovirus F40/41 in wastewater: Trends preceding, during, and following the non-A-to-E hepatitis outbreak in Stockholm

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