Single-cell RNA-sequencing reveals the transcriptional landscape of ND-42 mediated spermatid elongation via mitochondrial derivative maintenance in Drosophila testes

Integrative analysis of single-nucleus RNA-seq and bulk RNA-seq reveals germline cells development dynamics and niches in the Pacific oyster gonad

“…In single cell RNA-seq studies characterizing human and Drosophila spermatogenesis, it was discovered that male germ cell migration and spermatid elongation heavily rely on oxidative phosphorylation and mitochondrial ATP generation. 5 , 38 We also discovered that migration phase spermatocytes possessed upregulated ATP synthesis-related genes. This may be connected to the fact that in early gametogenic gonads, a small number of spermatocytes migrating toward the gonadal tubules are accompanied by the process of spermatocyte formation.…”

Single‐cell transcriptome characteristics of testicular terminal epithelium lineages during aging in the Drosophila

“…After filtering, 2114 high quality epithelial cells were retained, with a median of 1215.5 genes and 6099 unique unique molecular identifiers (UMIs) per cell. According to recently reported studies and our previously established marker genes (Witt et al., 2019 ; Yu, Fu, et al., 2023 ; Yu, Li, et al., 2023 ), we identified the testicular TE populations using MtnA gene expression. Testicular TE populations were further subdivided into six novel cell clusters (EP0–EP5).…”

“…Notably, several markers, e.g. Fasciclin III (Fas3), Shg, and hedgehog (hh), share similar expression patterns in hub cells and epithelial cell clusters (Raz et al., 2023 ; Witt et al., 2019 ; Yu, Fu, et al., 2023 ; Yu, Li, et al., 2023 ). Importantly, hh, Fas3, and Shg are highly enriched in hub cells and are required for stem cell maintenance in the niche (Lee et al., 2016 ; Michel et al., 2012 ; Voog et al., 2008 ).…”

“…Raw data of the 2 days and 10 days groups were obtained from previous studies (Yu, Fu, et al., 2023 ; Yu, Li, et al., 2023 ). An internal pipeline was used to process the raw reads of the 2, 10, and 40 Day groups to provide gene expression profiles.…”

“…Single‐cell transcriptome sequencing (scRNA‐seq) technology is used for the high‐throughput detection of transcription signatures at single‐cell resolution, and is widely used in studies of the reproductive system (Shi et al., 2021 ; Tan et al., 2023 ; Zhao et al., 2020 ). Recently, several scRNA‐seq studies were performed to generate a single‐cell atlas or cellular landscapes in different study models in Drosophila testis (Witt et al., 2019 ; Yu, Fu, et al., 2023 ; Yu, Li, et al., 2023 ). All these scRNA‐seq studies identified epithelial cell populations with under‐appreciated functions in testicular biology.…”