Single‐cell <scp>RNA</scp> sequencing analysis of the embryogenic callus clarifies the spatiotemporal developmental trajectories of the early somatic embryo in <i>Dimocarpus longan</i>

Zhang, Shuting; Zhu, Chen; Zhang, Xueying; Liu, Mengyu; Xue, Xiaodong; Lai, Chunwang; XuHan, Xu; Chen, Yukun; Zhang, Zihao; Zhang, Lai; Lin, Yuling

doi:10.1111/tpj.16319

Cited by 14 publications

(6 citation statements)

References 70 publications

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“…Transcription factors (TFs) are essential proteins that regulate cell fate decisions, developmental modes, and specific pathways (Shin et al., 2023; Zhang et al., 2023). They play pivotal roles in tissue differentiation, growth, development and response to environmental cues in both plants and animals (Weidemuller et al., 2021).…”

Section: Resultsmentioning

confidence: 99%

Environmental and molecular regulation of diapause formation in a scyphozoan jellyfish

Li,

Xu,

Sun

et al. 2023

Molecular Ecology

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Understanding the mechanisms underlying diapause formation is crucial for gaining insight into adaptive survival strategies across various species. In this study, we aimed to uncover the pivotal role of temperature and food availability in regulating diapausing podocyst formation in the jellyfish Aurelia coerulea. Furthermore, we explored the cellular and molecular basis of diapause formation using single‐cell RNA sequencing. Our results showed cell‐type‐specific transcriptional landscapes during podocyst formation, which were underscored by the activation of specific transcription factors and signalling pathways. In addition, we found that the heat shock protein‐coding genes HSC70 and HSP90a potentially act as hub genes that regulate podocyst formation. Finally, we mapped the single‐cell atlas of diapausing podocysts and identified cell types involved in metabolism, environmental sensing, defence and development that may collectively contribute to the long‐term survival and regulated excystment of diapausing podocysts. Taken together, the findings of this study provide novel insights into the molecular mechanisms that regulate diapause formation and contributes to a better understanding of adaptive survival strategies in a variety of ecological contexts.

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Section: Resultsmentioning

confidence: 99%

Environmental and molecular regulation of diapause formation in a scyphozoan jellyfish

Li,

Xu,

Sun

et al. 2023

Molecular Ecology

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“…Total RNA was isolated using the FastPure Plant Total RNA Isolation Kit (Vazyme) to serve as the template for the subsequent first-strand cDNA synthesis, which was finished using the PerfectStart Uni RT & qPCR Kit (TransGen). Three biological replicates were prepared for the quantitative real-time PCR (qRT-PCR) analysis using the Roche LightCycler 480 instrument and the PerfectStart Green qPCR Super-Mix (TransGen) as previously described by Zhang et al (2020Zhang et al ( , 2023.…”

Section: Total Rna Extraction and Gene Expression Analysismentioning

confidence: 99%

Role of hydrogen peroxide receptors in endocarp browning of postharvest longan fruit

Zhang,

Shan,

et al. 2024

Food Frontiers

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Longan (Dimocarpus longan) is an important subtropical evergreen fruit tree (family Sapindaceae) in southern China and exhibits a high commercial and nutritional value. Endocarp browning of longan fruit once harvested considerably reduces the commercial value. The excessive accumulation of reactive oxygen species (ROS) can induce the rapid endocarp browning of longan fruit during storage, but the ROS‐mediated mechanism remains relatively unclear. In this investigation, a hydrogen peroxide scavenger, 1,3‐dimethylthiourea (DMTU), was used to treat longan fruit to elucidate the physiological and molecular bases of the ROS‐mediated endocarp browning of longan fruit. Application of 5 mM DMTU significantly delayed endocarp browning, inhibited respiration rate, suppressed the accumulations of H2O2 and malondialdehyde (MDA), and activated the antioxidant system of longan fruit during storage, such as 3.8 of the DMTU‐treated fruit versus 1.6 of non‐DMTU‐treated fruit of the browning index, 398 versus 346 μg kg−1 S−2 of the respiration rate, and 47.5 versus 37.8 nmol g−1 of the MDA content after 6 days of storage. Furthermore, the DMTU treatment significantly affected the expression levels of the H2O2 receptors and hydrogen peroxide‐induced Ca2+ increases (HPCA)‐LIKEs (HPCALs). Additionally, the regulatory network comprising transcription factors controlling the HPCAL expression in longan was constructed. The major transcription factors, including GATA1, GATA28, TCP20, and AGL20, may contribute to the ROS‐mediated endocarp browning of longan fruit during storage by regulating the HPCAL expression levels. This study could provide new insights into the involvement of the HPCALs in the ROS‐mediated endocarp browning of longan fruit during storage.

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“…2016年, Hou等人 [26] 建立了三重组学测序技术(sin- 模式植物拟南芥 [30,31] , 农作物水稻 [32] 、玉米 [33,34] 为主, 未曾涉及药用植物的研究. 2021年及之后, 除了大豆 [35] 、草莓 [36] 、茶树 [37] 、木薯 [38] 、白菜 [39] 、杨树 [40] 等农作物以外, 在苜蓿 [41] 、长春花 [42] 、龙眼 [43] 、荆芥 [44] 等药用植物中也有了研究. 细胞核是细胞中复杂且高度特化的细胞器, 包含细胞的大部分遗传信息, 对于一些植物中特定组织来说, 分离单细胞核相对简单 [45] .…”

Section: 微流控技术的发展使单细胞测序技术得到了极大的提unclassified

单细胞测序在植物研究中的应用

An,

Zhang,

Tan

2024

Chin. Sci. Bull.

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Single‐cell RNA sequencing analysis of the embryogenic callus clarifies the spatiotemporal developmental trajectories of the early somatic embryo in Dimocarpus longan

Cited by 14 publications

References 70 publications

Environmental and molecular regulation of diapause formation in a scyphozoan jellyfish

Environmental and molecular regulation of diapause formation in a scyphozoan jellyfish

Role of hydrogen peroxide receptors in endocarp browning of postharvest longan fruit

单细胞测序在植物研究中的应用

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