“…In recent years, a large number of studies have been reported using single cell analysis to analyze individual tumor cells isolated from breast cancer (Navin et al, 2011; Deng et al, 2014; Wang et al, 2014; Eirew et al, 2015), colon cancer (Zong et al, 2012; Yu et al, 2014), pancreatic adenocarcinomas (Ruiz et al, 2011), muscle-invasive bladder cancer (Li et al, 2012b), intestinal cancer (Grün et al, 2015), lung adenocarcinoma cancer (Kim et al, 2015), renal cell carcinoma (Gerlinger et al, 2012; Li et al, 2012b), and acute myeloid leukemia (Ding et al, 2012; Hughes et al, 2014; Paguirigan et al, 2015). For example, Navin and colleagues investigated copy number variation in single tumor cells using DOP WGA followed by DNA sequencing to determine cell population structure and tumor evolution patterns in a single breast tumor (Navin et al, 2011).…”