Single-Cell Sequencing Revealed Pivotal Genes Related to Prognosis of Myocardial Infarction Patients

Abstract

Objectives. Myocardial infarction (MI) is a common cardiovascular disease. Histopathology is a main molecular characteristic of MI, but often, differences between various cell subsets have been neglected. Under this premise, MI-related molecular biomarkers were screened using single-cell sequencing. Methods. This work examined immune cell abundance in normal and MI samples from GSE109048 and determined differences in the activated mast cells and activated CD4 memory T cells, resting mast cells. Weighted gene coexpression network analysis (WGCNA) demonstrated that activated CD4 memory T cells were the most closely related to the turquoise module, and 10 hub genes were screened. Single-cell sequencing data (scRNA-seq) of MI were examined. We used t -distributed stochastic neighbor embedding ( t -SNE) for cell clustering. Results. We obtained 8 cell subpopulations, each of which had different marker genes. 7 out of the 10 hub genes were detected by single-cell sequencing analysis. The expression quantity and proportion of the 7 genes were different in 8 cell clusters. Conclusion. In general, our study revealed the immune characteristics and determined 7 prognostic markers for MI at the single-cell level, providing a new understanding of the molecular characteristics and mechanism of MI.

Inflammation and Oxidative Stress Role of S100A12 as a Potential Diagnostic and Therapeutic Biomarker in Acute Myocardial Infarction

“…Currently, the levels of myocardial enzyme (CKMB) and cardiac troponin I (cTnI) are still the gold standards for the clinical diagnosis of AMI, but they are not specific for AMI. Previous studies indicated that patients with heart failure, chronic kidney disease, and septicemia also have elevated cardiac troponin I (cTnI) levels, which may lead to false-positive results in AMI diagnosis [7,8]. Current studies have suggested that the circulating miRNAs, such as miRNA-499, miRNA-22, miR-223-3p, and miR-483-5p, could be used as potential biomarkers of AMI [9][10][11].…”

Single-Cell RNA Sequencing and Microarray Analysis Reveal the Role of Lipid-Metabolism-Related Genes and Cellular Immune Infiltration in Pre-Eclampsia and Identify Novel Biomarkers for Pre-Eclampsia

“…The initial step of the analysis involved preprocessing the scRNA-seq data using the “Seurat” package. This preprocessing encompassed several procedures, including assessing the proportion of mitochondrial genes using the PercentageFeatureSet function and conducting correlation analysis to explore relationships between sequencing depth, mitochondrial gene sequences, and total intracellular sequences [ 35 ].…”

Post-myocardial infarction fibrosis: Pathophysiology, examination, and intervention

“…Furthermore, CXCL10 and CXCL12 have leukocyte-independent mediatory effects, directly modulating CFs ( Chen and Frangogiannis, 2021 ). Additionally, single-cell sequencing has found different immune cell abundance (resting and activated mast cells, activated CD4 memory T cells) and high expression of chemokines in MI patients (CCL3, CXCL3, CXCL8, and CXCL16 in CD1C-CD141-dendritic cells and CCL4 and CCL5 in natural killer cells) ( Zhou et al, 2022 ).…”