Single‐cell transcriptome analysis reveals cellular and molecular alterations in small cell lung cancer tumors following chemotherapy

成晟,; Zhu, Guonian; Li, Yangqian; Wang, Haoyu; Wang, Suyan; Li, Chengping; Feng, Jiaming; Wang, Zhoufeng; Li, Wei Min

doi:10.1002/ijc.34629

Cited by 3 publications

References 48 publications

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The chromatin landscape of high-grade serous ovarian cancer metastasis identifies regulatory drivers in post-chemotherapy residual tumour cells

Croft,

Pounds,

Jeevan

et al. 2024

Commun Biol

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Disease recurrence following chemotherapy is a major clinical challenge in ovarian cancer (OC), but little is known regarding how the tumour epigenome regulates transcriptional programs underpinning chemoresistance. We determine the single cell chromatin accessibility landscape of omental OC metastasis from treatment-naïve and neoadjuvant chemotherapy-treated patients and define the chromatin accessibility profiles of epithelial, fibroblast, myeloid and lymphoid cells. Epithelial tumour cells display open chromatin regions enriched with motifs for the oncogenic transcription factors MEIS and PBX. Post chemotherapy microenvironments show profound tumour heterogeneity and selection for cells with accessible chromatin enriched for TP53, TP63, TWIST1 and resistance-pathway-activating transcription factor binding motifs. An OC chemoresistant tumour subpopulation known to be present prior to treatment, and characterised by stress-associated gene expression, is enriched post chemotherapy. Nuclear receptors RORa, NR2F6 and HNF4G are uncovered as candidate transcriptional drivers of these cells whilst closure of binding sites for E2F2 and E2F4 indicate post-treated tumour having low proliferative capacity. Delineation of the gene regulatory landscape of ovarian cancer cells surviving chemotherapy treatment therefore reveals potential core transcriptional regulators of chemoresistance, suggesting novel therapeutic targets for improving clinical outcome.

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The chromatin landscape of high-grade serous ovarian cancer metastasis identifies regulatory drivers in post-chemotherapy residual tumour cells

Croft,

Pounds,

Jeevan

et al. 2024

Commun Biol

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The underlying mechanism of cardiac injury in exertional heat stroke rats based on the scRNA-seq analysis

Luo,

Li,

Tang

et al. 2024

Preprint

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Background Heat dissipation relies on an intact cardiovascular system to dilate cutaneous blood vessels and increase cardiac output. However, the heart becomes a vulnerable organ and is susceptible to cardiac arrhythmias, functional failure, and focal myocardial necrosis in a hyperthermic heat-damaged state. In particular, people with cardiovascular dysfunction are at a much higher risk of exertional heat stroke (EHS). This study aimed to investigate and validate the cell signaling pathways and key genes associated with EHS by analyzing single-cell RNA sequencing (scRNA-seq) data from cardiac apical tissue of EHS rats. The findings are intended to elucidate the mechanisms underlying cardiac injury and to provide a theoretical basis for the early identification of biomarkers for cardiac injury in EHS. Results After exertional heat radiation, the heart's functionality was compromised. Annotation analysis revealed that the cell type and quantity did not differ between the EHS and control (CTL) groups. Cellchat analysis showed that the signal of EHS cardiac apex cells was enhanced in chemokine signaling pathway. The cardiac apical cells of the EHS group had the highest number of enriched genes in the oxidative stress pathway, according to GO/KEGG analysis of endothelial cells with the biggest proportion of cells. A total of 310 genes with changes in expression between the two groups were evaluated based on the Seurat-FindAllMarkers tools for all cell types. Of these, 18 genes with substantial variability were chosen for further verification. By using RT-qPCR verification, the expression differences of 12 genes were confirmed to be consistent with the above bioinformation analysis. Finally, Additional immunohistochemistry tests verified that Hspa8 and Hspe1 were up-regulated once more, while Id1, Ndufa4, and Cd36 were down-regulated. Conclusions The gene expression levels of Id1, Ndufa4, Cd36 were significantly reduced, and Hspa8, Hspe1 were significantly increased. These screened hypervariable genes play different roles in heat stress-induced mitochondrial and myocardial mechanical damage, protein misfolding, and they may become potential biomarkers in the mechanism of cardiac injury or keep an important link in the functional pathway of action described above.

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Multi-omics research progress in lung cancer

2024

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Lung cancer is a malignant tumor that seriously threatens human health, which has become one of the major health concerns that people care. Clinically, lung cancer is divided into small-cell lung cancer and non-small cell lung cancer, with non-small cell lung cancer accounting for 80% -85% of all pathological types of lung cancer. With the research on lung cancer, it has been found that most cases of lung cancer are already in the advanced stage, so early diagnosis of lung cancer is crucial. With the development of high-throughput technology, multi-omics analysis has provided us with a large amount of data for studying lung cancer. The study has discovered the important roles of genomics, transcriptomics, proteomics, and metabolomics in lung cancer. Based on the comprehensive analysis of multi-omics for lung cancer, we can identify the crucial biomarkers that are beneficial for early diagnosis of lung cancer for providing a better prognosis. This review provides the research progress of various omics technologies in lung cancer, so as to understand the molecular prognosis of lung cancer in clinical practice.

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Single‐cell transcriptome analysis reveals cellular and molecular alterations in small cell lung cancer tumors following chemotherapy

Cited by 3 publications

References 48 publications

The chromatin landscape of high-grade serous ovarian cancer metastasis identifies regulatory drivers in post-chemotherapy residual tumour cells

The chromatin landscape of high-grade serous ovarian cancer metastasis identifies regulatory drivers in post-chemotherapy residual tumour cells

The underlying mechanism of cardiac injury in exertional heat stroke rats based on the scRNA-seq analysis

Multi-omics research progress in lung cancer

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