Single-cell transcriptome mapping identifies a local, innate B cell population driving chronic rejection after lung transplantation

Smirnova, Natalia; Riemondy, Kent; Bueno, Marta; Collins, Susan; Suresh, Pavan; Wang, Xingan; Patel, Kapil; Königshoff, Mélanie; Sharma, Nirmal; Eickelberg, Oliver

doi:10.1172/jci.insight.156648

Cited by 9 publications

(8 citation statements)

References 72 publications

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“…10× Genomics Mice, human [136,139,140] Biomarker discovery: The scRNA-seq technique has been used to find gene expression signatures that can be considered biomarkers for predicting organ rejection or checking the responses to immunosuppressive therapy.…”

Section: Key Findings Methods/technologies Donor/recipient Species Re...mentioning

confidence: 99%

“…10× Genomics Mice, human [136,143] Macrophage polarization: Macrophages are found to be heterogeneous cell populations, which upon activation, polarize into various phenotypes. After transplantation, tissue-resident macrophages quickly change their gene expression profile into that of the host organ markers.…”

Section: Key Findings Methods/technologies Donor/recipient Species Re...mentioning

confidence: 99%

“…Because of chronic rejection, bronchiolitis obliterans syndrome is a big challenge and the key reason for weak lung transplantation outcomes. In a recent work [136], researchers used single-cell RNA sequencing to provide an atlas of bronchiolitis obliterans syndrome after lung transplantation outcomes. This atlas can be used to identify the changes in the cell compositions and their individual gene expression profiles during lung rejection.…”

Section: Lung Transplantationmentioning

confidence: 99%

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Single-Cell RNA Sequencing in Organ and Cell Transplantation

Abedini-Nassab,

Taheri,

Emamgholizadeh

et al. 2024

Biosensors

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Single-cell RNA sequencing is a high-throughput novel method that provides transcriptional profiling of individual cells within biological samples. This method typically uses microfluidics systems to uncover the complex intercellular communication networks and biological pathways buried within highly heterogeneous cell populations in tissues. One important application of this technology sits in the fields of organ and stem cell transplantation, where complications such as graft rejection and other post-transplantation life-threatening issues may occur. In this review, we first focus on research in which single-cell RNA sequencing is used to study the transcriptional profile of transplanted tissues. This technology enables the analysis of the donor and recipient cells and identifies cell types and states associated with transplant complications and pathologies. We also review the use of single-cell RNA sequencing in stem cell implantation. This method enables studying the heterogeneity of normal and pathological stem cells and the heterogeneity in cell populations. With their remarkably rapid pace, the single-cell RNA sequencing methodologies will potentially result in breakthroughs in clinical transplantation in the coming years.

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Section: Key Findings Methods/technologies Donor/recipient Species Re...mentioning

confidence: 99%

Section: Key Findings Methods/technologies Donor/recipient Species Re...mentioning

confidence: 99%

Section: Lung Transplantationmentioning

confidence: 99%

See 1 more Smart Citation

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Abedini-Nassab,

Taheri,

Emamgholizadeh

et al. 2024

Biosensors

View full text Add to dashboard Cite

show abstract

“…Numerous publications have analysed different immune cell profiles and their connection to lung allograft dysfunction [109][110][111]. BROSSEAU et al [110] demonstrated that LTRs with a frequency of CD9 + B-cells below 6.6% in peripheral blood had a significantly higher incidence of BOS.…”

Section: Lymphocyte Subset Assaysmentioning

confidence: 99%

“…However, these approaches face a major limitationan intrinsic bias determined by using prespecified surface markers. This might be aided by analysis of entire sets of transcripts by single-cell RNA sequencing [111]. Given the cost of the method and low clinical applicability, none of these approaches are yet ready for application in routine clinical practice, despite some promising results.…”

Section: Lymphocyte Subset Assaysmentioning

confidence: 99%

Molecular monitoring of lung allograft health: is it ready for routine clinical use?

Pradère,

Zajacova,

Bos

et al. 2023

Eur Respir Rev

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Maintenance of long-term lung allograft health in lung transplant recipients (LTRs) requires a fine balancing act between providing sufficient immunosuppression to reduce the risk of rejection whilst at the same time not over-immunosuppressing individuals and exposing them to the myriad of immunosuppressant drug side-effects that can cause morbidity and mortality. At present, lung transplant physicians only have limited and rather blunt tools available to assist them with this task. Although therapeutic drug monitoring provides clinically useful information about single time point and longitudinal exposure of LTRs to immunosuppressants, it lacks precision in determining the functional level of immunosuppression that an individual is experiencing. There is a significant gap in our ability to monitor lung allograft health and therefore tailor optimal personalised immunosuppression regimens. Molecular diagnostics performed on blood, bronchoalveolar lavage or lung tissue that can detect early signs of subclinical allograft injury, differentiate rejection from infection or distinguish cellular from humoral rejection could offer clinicians powerful tools in protecting lung allograft health. In this review, we look at the current evidence behind molecular monitoring in lung transplantation and ask if it is ready for routine clinical use. Although donor-derived cell-free DNA and tissue transcriptomics appear to be the techniques with the most immediate clinical potential, more robust data are required on their performance and additional clinical value beyond standard of care.

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Murine orthotopic lung transplant models: A comprehensive overview of genetic mismatch degrees and histopathological insights into chronic lung allograft dysfunction

Coppens,

Verleden,

Claes

et al. 2024

American Journal of Transplantation

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Single-cell transcriptome mapping identifies a local, innate B cell population driving chronic rejection after lung transplantation

Abstract: CO, USA) after they developed end-stage BOS. Paraffin-embedded samples of healthy lung tissue collected from organ donors served as controls.

Cited by 9 publications

References 72 publications

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Single-Cell RNA Sequencing in Organ and Cell Transplantation

Molecular monitoring of lung allograft health: is it ready for routine clinical use?

Murine orthotopic lung transplant models: A comprehensive overview of genetic mismatch degrees and histopathological insights into chronic lung allograft dysfunction

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