Single-cell transcriptomics of immune cells in lymph nodes reveals their composition and alterations in functional dynamics during the early stages of bubonic plague

Zhao, Yifan; Wang, Tong; Liu, Ziyang; Ke, Yuehua; Li, Ruoyan; Chen, Hongyan; You, Yang; Wu, Gengshan; Cao, Shiyang; Du, Zongmin; Bai, Fan; Yang, Ruifu

doi:10.1007/s11427-021-2119-5

Cited by 12 publications

(10 citation statements)

References 48 publications

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“…Y. pestis only circulates within a narrow host range between rodent reservoir hosts and f lea vectors in natural settings. The first challenge for Y. pestis survival in its lifecycle is sensing and adapting to temperature shifts while avoiding host innate immune cells during the early stage of infection and in host blood after release from innate immune cells, including macrophages, [27] is problematic as Y. pestis develops into a systemic infection. During the complex lifestyle of Y. pestis, the intense or even life-threatening environmental changes are concomitant with a series of dynamic regulatory physiologic responses.…”

Section: Unknown 1: Regulation Of Y Pestis Physiology In Vitro and In...mentioning

confidence: 99%

“…Recently, to describe the critical events occurring during the initial interactions between Y. pestis and the host innate immune cells, single-cell RNA sequencing (scR-NA-seq) was utilized to characterize the composition and alterations of immune cells in the functional dynamics of dLNs during the early stages of bubonic plague, which revealed a dynamic immune landscape at single-cell resolution of murine dLNs in response to Y. pestis infection [27]. The data suggested that DCs respond to the presence of Y. pestis within 2 hpi, followed by the activation of Mφs and monocytes (Mons) and recruitment of PMNs to dLNs at 24 hpi.…”

Section: Unknown 5: Early Interactions Between Y Pestis and The Host ...mentioning

confidence: 99%

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Yersinia pestis and Plague: Some Knowns and Unknowns

et al. 2023

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Since its first identification in 1894 during the third pandemic in Hong Kong, there has been significant progress in understanding the lifestyle of Yersinia pestis, the pathogen that is responsible for plague. Although we now have some understanding of the pathogen’s physiology, genetics, genomics, evolution, gene regulation, pathogenesis and immunity, there are many unknown aspects of the pathogen and its disease development. Here, we focus on some of the knowns and unknowns related to Y. pestis and plague. We notably focus on some key Y. pestis physiologic and virulence traits that are important for its mammal-flea-mammal life cycle, but also its emergence from the enteropathogen, Yersinia pseudotuberculosis. Some aspects of the genetic diversity of Y. pestis, the distribution and ecology of plague, as well as the medical countermeasures to protect our population are also provided. Lastly, we present some biosafety and biosecurity information related to Y. pestis and plague.

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Section: Unknown 1: Regulation Of Y Pestis Physiology In Vitro and In...mentioning

confidence: 99%

Section: Unknown 5: Early Interactions Between Y Pestis and The Host ...mentioning

confidence: 99%

Yersinia pestis and Plague: Some Knowns and Unknowns

et al. 2023

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“…The first challenge for Y. pestis survival in its lifecycle is sensing and adapting to temperature shifts while avoiding host innate immune cells during the early stage of infection and in host blood after release from innate immune cells, including macrophages. [ 86 ] This is problematic as Y. pestis develops into a systemic infection. During the complex lifestyle of Y. pestis , the intense or even life-threatening environmental changes are concomitant with a series of dynamic regulatory physiologic responses.…”

Section: The Evolution Of Y Pestismentioning

confidence: 99%

Overview of Yersinia pestis Metallophores: Yersiniabactin and Yersinopine

Chaaban

Mohsen²,

Ezzeddine

et al. 2023

Biology

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The pathogenic anaerobic bacteria Yersinia pestis (Y. pestis), which is well known as the plague causative agent, has the ability to escape or inhibit innate immune system responses, which can result in host death even before the activation of adaptive responses. Bites from infected fleas in nature transmit Y. pestis between mammalian hosts causing bubonic plague. It was recognized that a host’s ability to retain iron is essential in fighting invading pathogens. To proliferate during infection, Y. pestis, like most bacteria, has various iron transporters that enable it to acquire iron from its hosts. The siderophore-dependent iron transport system was found to be crucial for the pathogenesis of this bacterium. Siderophores are low-molecular-weight metabolites with a high affinity for Fe3+. These compounds are produced in the surrounding environment to chelate iron. The siderophore secreted by Y. pestis is yersiniabactin (Ybt). Another metallophore produced by this bacterium, yersinopine, is of the opine type and shows similarities with both staphylopine and pseudopaline produced by Staphylococcus aureus and Pseudomonas aeruginosa, respectively. This paper sheds light on the most important aspects of the two Y. pestis metallophores as well as aerobactin a siderophore no longer secreted by this bacterium due to frameshift mutation in its genome.

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“…Single-cell transcriptomics is indispensable for understanding cellular mechanisms of complex tissues and organisms (Guan et al, 2021;Jiang et al, 2021;Plass et al, 2018;Xie et al, 2021;Zhao et al, 2022). As single-cell technologies rapidly developed over recent years, its experimental throughput increased substantially, allowing to profile increasingly complex and diverse samples, and accumulating vast numbers of datasets over time.…”

Section: Introductionmentioning

confidence: 99%

Privacy-preserving integration of multiple institutional data for single-cell type identification with scPrivacy

Chen

Duan

Zhu

et al. 2022

Sci. China Life Sci.

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The rapid accumulation of large-scale single-cell RNA-seq datasets from multiple institutions presents remarkable opportunities for automatically cell annotations through integrative analyses. However, the privacy issue has existed but being ignored, since we are limited to access and utilize all the reference datasets distributed in different institutions globally due to the prohibited data transmission across institutions by data regulation laws. To this end, we present scPrivacy , which is the first and generalized automatically single-cell type identification prototype to facilitate single cell annotations in a data privacy-preserving collaboration manner. We evaluated scPrivacy on a comprehensive set of publicly available benchmark datasets for single-cell type identification to stimulate the scenario that the reference datasets are rapidly generated and distributed in multiple institutions, while they are prohibited to be integrated directly or exposed to each other due to the data privacy regulations, demonstrating its effectiveness, time efficiency and robustness for privacy-preserving integration of multiple institutional datasets in single cell annotations. Supporting Information The supporting information is available online at 10.1007/s11427-022-2224-4. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

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Single-cell transcriptomics of immune cells in lymph nodes reveals their composition and alterations in functional dynamics during the early stages of bubonic plague

Cited by 12 publications

References 48 publications

Yersinia pestis and Plague: Some Knowns and Unknowns

Yersinia pestis and Plague: Some Knowns and Unknowns

Overview of Yersinia pestis Metallophores: Yersiniabactin and Yersinopine

Privacy-preserving integration of multiple institutional data for single-cell type identification with scPrivacy

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