Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes

The precise delivery of targeted cells through magnetic field-driven microrobots/carriers is a promising technique for targeted therapy and tissue regeneration. This paper presents a microrobot designed with a burr-like porous spherical structure for carrying and delivering targeted cells in vivo under a magnetic gradient field-driven mechanism. The robot was fabricated by using three-dimensional laser lithography and coated with Ni for magnetic actuation and Ti for biocompatibility. Numerical and experimental studies demonstrated that the proposed microrobot design could enhance magnetic driving capability, promote cell-carrying capacity, and benefit cell viability. Microrobots loaded with cells could be automatically controlled to reach a desired site by using a self-constructed electromagnetic coil system, as verified by in vivo transport of cell-cultured microrobots in zebrafish embryos. The carried cells could be spontaneously released from the microrobot to the surrounding tissues; in vitro experiments showed that cells from the microrobot were directly released onto the desired site or were able to pass through the blood vessel-like microchannel to arrive at the delivery area. Further in vivo cell-releasing tests were performed on nude mice, followed by histological study. This research provides a microrobotic device platform for regenerative medicine and cell-based therapy.

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“…Last, the cell-cultured microrobot was detached by a home-designed micromanipulator ( fig. S5) (50,51).…”

Section: Cell Culture On Microrobotmentioning

confidence: 99%

Development of a magnetic microrobot for carrying and delivering targeted cells

et al. 2018

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“…AC, DC or pulsed electric fields (PEFs) are adopted for the electromanipulation of biological cells in various applications, such as dielectrophoresis, electrorotation, electrodeformation, electrodisruption and electroporation [1]. Several methods including mechanical (microinjection), chemical (lipofection reagents), biological (viral vectors) and physical (sonoporation, magnetofection, electroporation) phenomena have been developed to facilitate the transport of molecules through the cell membrane [2]. In particular, during the past four decades electroporation (EP) technique has gained increasing importance in biology and in medicine [3].…”

Section: Introductionmentioning

confidence: 99%

Design of Electroporation Process in Irregularly Shaped Multicellular Systems

Mescia

Chiapperino

Bia³

et al. 2019

Electronics

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Electroporation technique is widely used in biotechnology and medicine for the transport of various molecules through the membranes of biological cells. Different mathematical models of electroporation have been proposed in the literature to study pore formation in plasma and nuclear membranes. These studies are mainly based on models using a single isolated cell with a canonical shape. In this work, a space–time (x,y,t) multiphysics model based on quasi-static Maxwell’s equations and nonlinear Smoluchowski’s equation has been developed to investigate the electroporation phenomenon induced by pulsed electric field in multicellular systems having irregularly shape. The dielectric dispersion of the cell compartments such as nuclear and plasmatic membranes, cytoplasm, nucleoplasm and external medium have been incorporated into the numerical algorithm, too. Moreover, the irregular cell shapes have been modeled by using the Gielis transformations.

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“…Microcapillary pipettes are ideal for single cell manipulation, such as cell picking and loading. Submicron‐sized capillary tubes allow microinjection and delivery of exogeneous materials into cells by piecing through cell membranes 8, 9, 10, 11, 12. The long capillary filled with aqueous liquid can also function as an electrode for measuring cell properties or performing electroporation 13, 14.…”

Section: Introductionmentioning

confidence: 99%

Liquid Metal‐Based Multifunctional Micropipette for 4D Single Cell Manipulation

et al. 2018

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A novel manufacturing approach to fabricate liquid metal‐based, multifunctional microcapillary pipettes able to provide electrodes with high electrical conductivity for high‐frequency electrical stimulation and measurement is proposed. 4D single cell manipulation is realized by applying multifrequency, multiamplitude, and multiphase electrical signals to the microelectrodes near the pipette tip to create 3D dielectrophoretic trap and 1D electrorotation, simultaneously. Functions such as single cell trapping, patterning, transfer, and rotation are accomplished. Cell viability and multiday proliferation characterization has confirmed the biocompatibility of this approach. This is a simple, low‐cost, and fast fabrication process that requires no cleanroom and photolithography step to manufacture 3D microelectrodes and microchannels for easy access to a wide user base for broad applications.

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Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes

Abstract: Cell transfection is

Cited by 93 publications

References 32 publications

Development of a magnetic microrobot for carrying and delivering targeted cells

Development of a magnetic microrobot for carrying and delivering targeted cells

Design of Electroporation Process in Irregularly Shaped Multicellular Systems

Liquid Metal‐Based Multifunctional Micropipette for 4D Single Cell Manipulation

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