Single-chain polymer nanoparticles: Mimic the proteins

Huo, Meng; Wang, Niejun; Fang, Tommy; Sun, Mengzhen; Wei, Yen; Yuan, Jinying

doi:10.1016/j.polymer.2015.04.011

Cited by 59 publications

(58 citation statements)

References 90 publications

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“…Consequently, SCPNs have gained interest as potential mimetics of biomacromolecules such as proteins 5,6 and for application in different fields including nanomedicine.…”

Section: Synthesis and Functionalization Of Dextran-based Single-chaimentioning

confidence: 99%

“…[2][3][4] SCPNs based on synthetic polymers benefit from the possibility of a controlled construction of the precursors in order to prepare tuned SCPNs with desired size and functionality.3 Additionally, a wide variety of biocompatible, non-toxic and ready-to-use natural polymers are available. Consequently, SCPNs have gained interest as potential mimetics of biomacromolecules such as proteins 5,6 and for application in different fields including nanomedicine. [7][8][9] Among natural polymers, polysaccharides can be envisaged as natural analogues of polyethylene glycol (PEG).…”

Section: Synthesis and Functionalization Of Dextran-based Single-chaimentioning

confidence: 99%

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Synthesis and functionalization of dextran-based single-chain nanoparticles in aqueous media

et al. 2017

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Synthesis and Functionalization of Dextran-Based Single-Chain Nanoparticles in Aqueous Media IK4-CIDETEC, Pº Miramón 196, 20014, Donostia-San Sebastián, Spain. b. Radiochemistry and Nuclear Imaging Group, CIC biomaGUNE, Pº Miramón 182, 20014, Donostia-San Sebastián, Spain. Water-dispersible dextran-based single-chain polymer nanoparticles (SCPNs) were prepared in aqueous media and mild conditions. Radiolabeling of the resulting biocompatible materials allowed the study of lung deposition of aqueous aerosols after intratracheal nebulization by means of single-photon emission computed tomography (SPECT), demostrating their potential use as imaging contrast agents.Advances in engineering polymer chains at the molecular level 1 have pushed the development of synthetic strategies for the controlled compaction of single polymer coils into unimolecular soft nano-objects, named single-chain polymer nanoparticles (SCPNs).2-4 SCPNs based on synthetic polymers benefit from the possibility of a controlled construction of the precursors in order to prepare tuned SCPNs with desired size and functionality.3 Additionally, a wide variety of biocompatible, non-toxic and ready-to-use natural polymers are available. Consequently, SCPNs have gained interest as potential mimetics of biomacromolecules such as proteins 5,6 and for application in different fields including nanomedicine.7-9 Among natural polymers, polysaccharides can be envisaged as natural analogues of polyethylene glycol (PEG). For example, dextrans have been used in several biomedical applications due to aqueous solubility, biocompatibility, biodegradability, wide availability, ease of modification and non-fouling properties.10,11 However, in vivo application of SCPNs can be limited due to their small size. Rapid blood clearance is expected after intravenous administration of particles below 5 nm size. 12 In the last years, non-invasive lung administration route has been broadly studied, 13 and SCPNs could be beneficial due to their small size.14 Synthetic routes to SCPNs are mainly based on intrachain homo-and hetero-coupling or cross-linking-induced collapseof pre-functionalized polymers through covalent, dynamic covalent, and non-covalent bonding. 15,16 Most of the covalent strategies are performed in organic solvents and require highly diluted polymer solutions (usually <1 mg/mL), high temperatures and/or the presence of metal catalysts. The preparation of SCPNs through "continuous addition" avoids ultra-dilution conditions, which is beneficial for multigram scale preparations. 17 Recently, it has been described that the presence of oligo(ethylene glycol) brushes as side-chains allows to achieve SCPNs at high polymer concentrations (100 mg/mL). 18 However, the development of general procedures to obtain functionalizable SCPNs in aqueous media, mild conditions and scalable conditions is still a challenging issue.Our group reported a strategy to generate structurally defined and water-dispersible poly(methacrylic acid)-based SCPNs.19 In pursuit of novel types of...

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“…Consequently, SCPNs have gained interest as potential mimetics of biomacromolecules such as proteins 5,6 and for application in different fields including nanomedicine.…”

Section: Synthesis and Functionalization Of Dextran-based Single-chaimentioning

confidence: 99%

Section: Synthesis and Functionalization Of Dextran-based Single-chaimentioning

confidence: 99%

Synthesis and functionalization of dextran-based single-chain nanoparticles in aqueous media

et al. 2017

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“…[8][9][10][11][12][13][14] Although the design and synthesis of single chain objects has recently received great attention, 15 the development in this field is still in its initial phase. So far, several types of strategies to mediate the single chain collapse to form SCNPs have been explored, [16][17][18][19][20][21][22] ranging from hydrogen bonding, 23-27, 10, 28-31 covalent bonding, [32][33][34][35][36] to dynamic covalent bonding. [37][38][39][40] All these recent advances have provided versatile approaches to induce the folding of a single polymer chain.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Sequence-Controlled Multiblock Single Chain Nanoparticles by a Stepwise Folding–Chain Extension–Folding Process

et al. 2016

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Copyright and reuse:The Warwick Research Archive Portal (WRAP) makes this work by researchers of the University of Warwick available open access under the following conditions. Copyright © and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable the material made available in WRAP has been checked for eligibility before being made available.Copies of full items can be used for personal research or study, educational, or not-for profit purposes without prior permission or charge. Provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. Publisher's statement:"This document is the Accepted Manuscript version of a Published Work that appeared in final form in Macromolecules copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work http://pubs.acs.org/page/policy/articlesonrequest/index.html ." A note on versions:The version presented here may differ from the published version or, version of record, if you wish to cite this item you are advised to consult the publisher's version. Please see the 'permanent WRAP URL above for details on accessing the published version and note that access may require a subscription. ABSTRACTThe specific activity of proteins can be traced back to their highly defined tertiary structure, which is a result of a perfectly controlled intra-chain folding process. In the herein presented work the folding of different distinct domains within a single macromolecule is demonstrated. RAFT polymerization was used to produce multi-block copolymers, which are decorated with pendant hydroxyl groups in foldable sections, separated by non-functional spacer blocks in between. OH-bearing blocks were folded using an isocyanate cross linker prior to chain extension to form single chain nanoparticles (SCNP). After addition of a spacer block and a further OH decorated block, folding was repeated to generate individual SCNP within a polymer chain. Control experiments were performed indicating the absence of inter block cross linking. SCNP were found to be condensed by a combination of covalent and supra molecular (hydrogen bonds) linkage. The approach was used to create a highly complex penta-block copolymer having three individually folded subdomains with an overall dispersity of 1.21. The successful formation of SCNP was confirmed by size exclusion chromatography (SEC), nuclear magnetic resonance (NMR), differential scanning calorimetry (DSC) and atomic force microscopy (AFM).

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“…Single‐chain folding is also an effective method to simulate biomacromolecules. Especially, to achieve single‐chain folding via intramolecular noncovalent interaction is analogous to many folding process in nature, and it is envisaged as a simple pattern to mimic the behavior of biomacromolecules such as proteins, which exist in a well‐defined secondary, tertiary, and quaternary structure, resulting from accurate molecular weight, tacticity, monomer sequence, and so forth . Diverse crosslinking strategies, including covalent bonding, dynamic covalent bonding, and supramolecular interaction, have been adopted to achieve intramolecular collapsing.…”

Section: Introductionmentioning

confidence: 99%

Supramolecular Nanoparticles via Single-Chain Folding Driven by Ferrous Ions

Wang

Jin

et al. 2016

Macromol. Rapid Commun.

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Single-chain nanoparticles can be obtained via single-chain folding assisted by intramolecular crosslinking reversibly or irreversibly. Single-chain folding is also an efficient route to simulate biomacromolecules. In present study, poly(N-hydroxyethylacrylamide-co-4'-(propoxy urethane ethyl acrylate)-2,2':6',2''-terpyridine) (P(HEAm-co-EMA-Tpy)) is synthesized via reversible addition fragmentation chain transfer polymerization. Single-chain folding and intramolecular crosslinking of P(HEAm-co-EMA-Tpy) are achieved via metal coordination chemistry. The intramolecular interaction is characterized on ultraviolet/visible spectrophotometer (UV-vis spectroscopy), proton nuclear magnetic resonance ((1)H NMR), and differential scanning calorimetry (DSC). The supramolecular crosslinking mediated by Fe(2+) plays an important role in the intramolecular collapsing of the single-chain and the formation of the nanoparticles. The size and morphology of the nanoparticles can be controlled reversibly via metal coordination chemistry, which can be characterized by dynamic light scattering (DLS), transmission electron microscope (TEM), and atomic force microscope (AFM).

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Single-chain polymer nanoparticles: Mimic the proteins

Cited by 59 publications

References 90 publications

Synthesis and functionalization of dextran-based single-chain nanoparticles in aqueous media

Synthesis and functionalization of dextran-based single-chain nanoparticles in aqueous media

Synthesis of Sequence-Controlled Multiblock Single Chain Nanoparticles by a Stepwise Folding–Chain Extension–Folding Process

Supramolecular Nanoparticles via Single-Chain Folding Driven by Ferrous Ions

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