Single-channel recording in brain slices reveals heterogeneity of nicotinic receptors on individual neurons within the chick lateral spiriform nucleus

Weaver, William R.; Chiappinelli, Vincent A.

doi:10.1016/0006-8993(96)00391-5

Cited by 9 publications

(6 citation statements)

References 41 publications

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“…Nicotinic events were also blocked by ␣-cobrotoxin (100 -200 nM, n ϭ 7), whereas ␣-bungarotoxin (100 -200 nM, n ϭ 6) had no effect, even if applied for Ͼ30 min. This indicates that the nicotinic receptor subtypes expressed by developing cortical neurons are not the ␣7-9 subunit-containing receptors that are blocked by ␣-bungarotoxin (Role and Berg, 1996;Weaver and Chiapinelli, 1996). Spontaneous cholinergic synaptic currents reversed between ϩ15 and ϩ40 mV (mean, ϩ23.8 mV; SEM 8.6 mV; n ϭ 4; Fig.…”

Section: Acetylcholine and Serotonin Mediate Fast Spontaneous Synaptimentioning

confidence: 88%

Fast Synaptic Signaling by Nicotinic Acetylcholine and Serotonin 5-HT₃Receptors in Developing Visual Cortex

1997

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Cholinergic and serotonergic fiber systems invade the developing visual cortex several weeks before eye opening; both transmitters have been implicated in plasticity of neocortical circuits. These transmitters have been presumed to act predominantly through second messenger-coupled receptors, because fast cholinergic or serotonergic neurotransmission has never been observed in neocortex. However, acetylcholine and serotonin also act on ligand-gated ion channels; the nicotinic acetylcholine receptor and the serotonin 5-HT 3 receptor, respectively. Here, using whole-cell patch-clamp techniques in developing ferret visual cortex, we pharmacologically isolated fast, spontaneous, and evoked cholinergic and serotonergic synaptic events in pyramidal cells and interneurons of all cortical layers. The number of cells receiving such inputs increased with the ingrowth of thalamic afferents, and the frequencies of the spontaneous events increased at eye opening. Thus, both acetylcholine and serotonin can mediate fast synaptic transmission in the visual cortex; the early onset of these mechanisms suggests a role during initial stages of circuit formation and during subsequent experience-dependent remodeling of cortical connections.

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Section: Acetylcholine and Serotonin Mediate Fast Spontaneous Synaptimentioning

confidence: 88%

Fast Synaptic Signaling by Nicotinic Acetylcholine and Serotonin 5-HT₃Receptors in Developing Visual Cortex

1997

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“…Buisson and Bertrand (2001) reported that there were two types of conductance levels in ␣4␤2 AChRs expressed in HEK293 cells with the conductances 38.8 pS and 43.4 pS (high conductance type) and 16.7 pS and 23.5 pS (low conductance type). Several other studies reported multiple conductance levels of currents in neuronal nicotinic AChRs (Bormann and Matthaei, 1983;Weaver and Chiappinelli, 1996).…”

Section: Two Conductance Components Of ␣4␤2 Achrsmentioning

confidence: 95%

Single‐Channel Analyses of Ethanol Modulation of Neuronal Nicotinic Acetylcholine Receptors

Zuo

Nagata

Yeh

et al. 2004

Alcoholism Clin & Exp Res

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“…Fifth, single-channel study of the muscle receptor showed a very high open probability [P open ϭ ␤/(␣ϩ␤)] of about 0.98 (Colquhoun and Sakmann, 1985). Previous data suggest that the nnAChRs have a much lower open probability, although no maximum P open value is available (Weaver and Chiappinelli, 1996). Because no such data are available, we chose 0.67 for P open of ACh-opened channels, as in our previous simulations .…”

Section: Jpetaspetjournalsorgmentioning

confidence: 99%

Dual Action of n-Butanol on Neuronal Nicotinic α4β2 Acetylcholine Receptors

Zuo

Yeh²,

Narahashi³

2002

J Pharmacol Exp Ther

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n-Alcohols exert a dual action on neuronal nicotinic acetylcholine (ACh) receptors with short-chain alcohols exhibiting potentiating action and long-chain alcohols exhibiting inhibitory action. n-Butanol lies at the transition point from potentiation to inhibition. To elucidate the mechanism of dual action of alcohols, the effects of n-butanol on the human ␣4␤2 ACh receptors expressed in the HEK293 cell line were analyzed in detail by the whole-cell patch-clamp technique. Prolonged applications of n-butanol evoked small currents with an EC 50 value of 230 Ϯ 90 mM and a Hill coefficient of 1.8 Ϯ 0.4. This current was blocked by either the ACh channel blocker mecamylamine or the receptor blocker dihydro-␤-erythroidine, indicating that butanol activated receptors as a partial agonist. As expected from its partial agonist action, n-butanol also modulated AChinduced currents in a concentration-dependent manner. Butanol at 300 mM potentiated currents induced by low concentrations of ACh (Յ30 M), while inhibiting the currents induced by high concentrations of ACh (100 -3000 M). In addition, butanol at a low concentration (10 mM) suppressed the currents evoked by 10 to 3000 M ACh, a result consistent with a channel-blocking action. Most features of n-butanol effects were satisfactorily simulated by a model in which butanol acts as a partial agonist and as a channel blocker.

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Single-channel recording in brain slices reveals heterogeneity of nicotinic receptors on individual neurons within the chick lateral spiriform nucleus

Cited by 9 publications

References 41 publications

Fast Synaptic Signaling by Nicotinic Acetylcholine and Serotonin 5-HT₃Receptors in Developing Visual Cortex

Fast Synaptic Signaling by Nicotinic Acetylcholine and Serotonin 5-HT₃Receptors in Developing Visual Cortex

Single‐Channel Analyses of Ethanol Modulation of Neuronal Nicotinic Acetylcholine Receptors

Dual Action of n-Butanol on Neuronal Nicotinic α4β2 Acetylcholine Receptors

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Single-channel recording in brain slices reveals heterogeneity of nicotinic receptors on individual neurons within the chick lateral spiriform nucleus

Cited by 9 publications

References 41 publications

Fast Synaptic Signaling by Nicotinic Acetylcholine and Serotonin 5-HT3Receptors in Developing Visual Cortex

Fast Synaptic Signaling by Nicotinic Acetylcholine and Serotonin 5-HT3Receptors in Developing Visual Cortex

Single‐Channel Analyses of Ethanol Modulation of Neuronal Nicotinic Acetylcholine Receptors

Dual Action of n-Butanol on Neuronal Nicotinic α4β2 Acetylcholine Receptors

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Fast Synaptic Signaling by Nicotinic Acetylcholine and Serotonin 5-HT₃Receptors in Developing Visual Cortex

Fast Synaptic Signaling by Nicotinic Acetylcholine and Serotonin 5-HT₃Receptors in Developing Visual Cortex