Abstract-This paper addresses the problem of single channel speech separation to extract and enhance the desired speech signals from mixed speech signals. We propose a new speech separation algorithm by utilizing Bayesian approach for the case in which, underlying sources are mixed at different levels of energies. This situation is not considered in many single channel speech separation methods. To validate the effectiveness of our proposed method, it is compared with a state-of-the art method which is a gain adapted Maximum Likelihood estimator. Through the experiments, we show that our proposed method outperforms the compared method.Index Terms-Single channel speech separation, bayesian approach, gain estimation.