Single-copy sequence hybridizes to polymorphic and homologous loci on human X and Y chromosomes.

Page, David C.; Martinville, B de; Barker, David F.; Wyman, Arlene R.; White, Ray; Francke, Uta; Botstein, David

doi:10.1073/pnas.79.17.5352

Cited by 150 publications

(45 citation statements)

References 16 publications

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“…This same genomic clone also appears to hybridize to DNA located within the distal end of the Y chromosome long arm. The hybridization pattern of this clone, which detects X and Y chromosome homology, is in contrast to that found at another DNA locus on the X chromosome, DXYS1 (34) (Figure 5) and is localized to Yq. Analysis of this cDNA clone HeLa #71 has led to the study of three different chromosomes: an autosomal region, the X chromosome and the Y chromosome.…”

Section: Discussionmentioning

confidence: 60%

Identification and isolation of transcribed human X chromosome DNA sequences

et al. 1983

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A human X chromosome specific phage library has been used as a source of X-specific genomic DNA clones which hybridize with cellular RNA. Random cDNA clones were mapped for X chromosome sequence localization and 8 were identified as hybridizing to X chromosome Hind III fragments. All eight also hybridized with autosomal Hind III fragments. The X chromosome genomic sequences corresponding to two of these cDNA clones were isolated from a phage library constructed with the Hind III endonuclease digest products of X enriched DNA. One genomic DNA segment, localized to the short arm of the X, shared sequence homology with at least one region of the human Y chromosome. The methodology developed represents a rapid means to obtain a specific genomic DNA clone from a single chromosome when multiple different genomic loci homologous to an expressed DNA sequence exist.INTRODUCTION:

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Section: Discussionmentioning

confidence: 60%

Identification and isolation of transcribed human X chromosome DNA sequences

et al. 1983

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“…In these investigations (3,6,7,(23)(24)(25), several families of repetitive sequences have been characterized which together compose up to 70% of the total Y chromosomal DNA. In addition, some unique sequences have been described which hybridize to sequences on the Y chromosome (10,34).…”

Section: Discussionmentioning

confidence: 99%

“…Characterization and chromosomal localization of repetitive and unique sequences located on the Y. It may be assumed (5,10,31,(33)(34)(35) that, for a consider- It has been speculated (5,191 that the Y chromosome may contain a number of genes in addition to those associated with primary sexual development (probably located in the distal part of its short arm (5)). According to these ideas, these genes might take part in crossing over events with homologous X chromosomal regions.…”

Section: Discussionmentioning

confidence: 99%

Preparative dual‐beam sorting of the human Y chromosome and in situ hybridization of cloned DNA probes

Cremer¹,

Rappold²,

Gray³

et al. 1984

Cytometry

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Bivariate Hoechstkhromomycin flow karyotypes for chromosomes from a Chinese hamster-human hybrid cell line (CH-Y-VII) were established that have retained a human Y chromosome. These bivariate flow karyotypes showed the human Y chromosome to be completely separated from the peaks for the Chinese hamster chromosomes. In preparative dual-beam sorting experiments, 3 x lo6 chromosomes were sorted from the Y peak into frozen petri dishes. An examination of Qbanded samples of sorted chromosomes revealed that 82% * 5% of them were human Y chromosomes.The DNA from the sorted chromosomes (approximately 250 ng) was isolated and used to establish a genomic library (vector X gt WESAB). Three clones (YACG 45, 52, 54) of Genomic libraries representative of the DNA of a specific chromosome (chromosome specific genomic library) may have a wide range of applications; e.g., for an analysis of the correlation of specific genetic traits with polymorphisms of the length of restriction fragments (4, 11,21,32), for establishment of DNA probes specific for particular chromosomes or chromosome regions (32,38), for investigations of tumor-related genetic occurrences (ll), or for studies concerning chromosome evolution (7,25). In principle, such investigations may also be performed using a library of the entire genome; however, the availability of chromosome-specific libraries should considerably facilitate them.The most direct way to construct a chromosome-specific library is to purify the chromosome of interest by fluorescence activated sorting (20) and to establish a genomic library from the DNA of the sorted chromosomes. This approach has recently been used to establish a genomic library for DNA from the X chromosomes this library containing inserts of repetitive human DNA were used for chromosomal localization by means of in situ hybridization to metaphase spreads of male human lymphocytes and of CH-Y-VII cells. In all three cases, a significant binding to the human Y chromosome was observed. A more detailed study of the chromosomal distribution of sequences homologous to the insert of YACG 45 suggested the existence of minor binding sites on several human autosomes. Southern blot analysis revealed the existence of other human specific sequences without Y chromosome specificity.Key terms: Isolated chromosomes, chromosome sorting, genomic libraries, in situ hybridization from man (12) and mouse (261, and from human chromosomes 21 and 22 (22), respectively.In this paper, we report on the preparative dual-beam sorting of the human Y chromosome for the construction of a Y chromosomal genomic library and a characterization of the Y specificity of cloned DNA probes. The human Y chromosomes (in this context also simply referred to as "Y chromosomes") were purified by dualbeam sorting (13,141 from a Chinese hamster-man hybrid cell line retaining the Y as the only free human chromosome (9).

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“…Most well-described DNA sequence polymorphisms involve single nucleotide changes, or small insertions or deletions, which result in the presence or absence of a particular restriction enzyme recognition site (18)(19)(20)(21). In another type of polymorphism, a large segment of DNA of unknown function varies widely in length among individuals (22,23).…”

Section: Introductionmentioning

confidence: 99%

Origin of cell populations after bone marrow transplantation. Analysis using DNA sequence polymorphisms.

et al. 1985

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After successful bone marrow transplantation, patient hematopoietic and lymphoid cells are replaced by cells derived from the donor marrow. To document and characterize successful engraftment, host and donor cells must be distinguished from each other. We have used DNA sequence polymorphism analysis to determine reliably the host or donor origin of posttransplant cell populations. Using a selected panel of six cloned DNA probes and associated sequence polymorphisms, at least one marker capable of distinguishing between a patient and his sibling donor can be detected in over 95% of cases. Posttransplant patient peripheral leukocytes were examined by DNA restriction enzyme digestion and blot hybridization analysis. We have studied 18 patients at times varying from 13 to 1,365 d after marrow transplantation. Mixed lymphohematopoietic chimerism was detected in 3 patients, with full engraftment documented in 15. One patient with severe combined immunodeficiency syndrome was demonstrated to have T cells of purely donor origin, with granulocytes and B cells remaining of host origin. Posttransplant leukemic relapse was studied in one patient and shown to be of host origin. DNA analysis was of particular clinical value in three cases where failure of engraftment or graft loss was suspected. In two of the three cases, full engraftment was demonstrated and in the third mixed lymphohematopoietic chimerism was detected. DNA sequence polymorphism analysis provides a powerful tool for the documentation of engraftment after bone marrow transplantation, for the evaluation of posttransplant lymphoma or leukemic relapse, and for the comprehensive study of mixed hematopoietic and lymphoid chimeric states.

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Single-copy sequence hybridizes to polymorphic and homologous loci on human X and Y chromosomes.

Cited by 150 publications

References 16 publications

Identification and isolation of transcribed human X chromosome DNA sequences

Identification and isolation of transcribed human X chromosome DNA sequences

Preparative dual‐beam sorting of the human Y chromosome and in situ hybridization of cloned DNA probes

Origin of cell populations after bone marrow transplantation. Analysis using DNA sequence polymorphisms.

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