Single count of radicle emergence and mean germination time estimate seed vigour of Chinese milk vetch (<i>Astragalus sinicus</i>)

Tao, Qibo; Sun, Jiawei; Zhang, Yaqi; Sun, Xiaotong; Li, Zhenyi; Zhong, Shangzhi; Sun, Juan

doi:10.15258/sst.2022.50.1.06

Cited by 8 publications

(9 citation statements)

References 37 publications

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“…All seed lots used in the present experiment were from a single cultivar; thus, any differences in their germination and emergence patterns and stress tolerance were not due to their genotypes. Therefore, from a seed physiology perspective, the difference in the above traits between seed lots was most likely due to different vigor levels caused by seed aging and deterioration [18]. From the start of seed imbibition to germination or emergence, metabolic repair, including DNA repair, is required and is a necessary step for the subsequent events leading to germination and emergence [55].…”

Section: Discussionmentioning

confidence: 99%

“…Previous research has suggested the presence of a positive linear relationship between seed vigor and seedling emergence in the field [16,17]. Tao et al found that there was an advantage in using high-vigor CMV seeds to achieve better seedling emergence, whereas low-vigor seeds caused limited seedling emergence [18]. The selection of high-vigor seeds has also been proven to be helpful for seedling emergence in other forage legumes, such as alfalfa (Medicago sativa L.) [19], white clover (Trifolium repens L.) [20], and birdsfoot trefoil (Lotus corniculatus L.) [21].…”

Section: Introductionmentioning

confidence: 99%

“…There are many ways to test seed vigor, including electrical conductivity [24], DNA replication [25], accelerated aging [26], controlled deterioration [27], and radicle emergence [14,28]. Previously, Tao et al indicated that an individual count of radicle emergence may have the potential for CMV seed-vigor testing [18]. The methods based on germination characteristics under stress conditions also have the potential for seed-vigor testing.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hydrotime Model Parameters Estimate Seed Vigor and Predict Seedling Emergence Performance of Astragalus sinicus under Various Environmental Conditions

Tao

Chen

Bai

et al. 2023

Plants

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Seed vigor is an important aspect of seed quality. High-vigor seeds show rapid and uniform germination and emerge well, especially under adverse environmental conditions. Here, we determined hydrotime model parameters by incubating seeds at different water potentials (0.0, −0.2, −0.4, −0.6, and −0.8 MPa) in the laboratory, for 12 seed lots of Chinese milk vetch (Astragalus sinicus) (CMV), a globally important legume used as forage, green manure, and a rotation crop. Pot experiments were conducted to investigate the seedling emergence performance of 12 CMV seed lots under control, water stress, salinity stress, deep sowing, and cold stress conditions. Meanwhile, the field emergence performance was evaluated on two sowing dates in June and October 2022. Correlation and regression analyses were implemented to explore the relationships between hydrotime model parameters and seedling emergence performance under various environmental conditions. The seed germination percentage did not differ significantly between seed lots when seeds were incubated at 0.0 MPa, whereas it did differ significantly between seed lots at water potentials of −0.2, −0.4, and −0.6 MPa. The emergence percentage, seedling dry weight, and simplified vigor index also differed significantly between the 12 seed lots under various environmental conditions. Ψb(50) showed a significant correlation with germination and emergence performance under various environmental conditions; however, little correlation was observed between θH or σφb and germination and emergence. These results indicate that Ψb(50) can be used to estimate seed vigor and predict seedling emergence performance under diverse environmental conditions for CMV and similar forage legumes. This study will enable seed researchers, plant breeders, and government program directors to target higher seed vigor more effectively for forage legumes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Hydrotime Model Parameters Estimate Seed Vigor and Predict Seedling Emergence Performance of Astragalus sinicus under Various Environmental Conditions

Tao

Chen

Bai

et al. 2023

Plants

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“…Seed vigor testing is an important component of quality assurance in crop and forage seed production [23,24]. The traditional standard germination (SG) test, conducted according to the International Seed Testing Association, is generally used to evaluate seed quality [25].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, the mean germination time (MGT) of pant seed lots determined during RE test in the laboratory was found to be related to FE [39,40]. In low-vigor seeds, the RE is delayed, and evidence has indicated that the vigor level determines the length of this delay, which has been referred to as the lag period and described by the MGT [24]. Thus, the test based on the measurement of MGT also has great potential as an efficient way to test seed vigor [15].…”

Section: Introductionmentioning

confidence: 99%

Siberian Wildrye (Elymus sibiricus) Seed Vigor Estimation for the Prediction of Emergence Performance under Diverse Environmental Conditions

Tao,

Xing,

Meng

et al. 2024

Agronomy

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Seed vigor is an important aspect of seed quality. It is critical to predict seed vigor and plant seedling emergence under diverse environmental conditions using the laboratory vigor test. Accordingly, laboratory experiments were conducted to determine the standard germination (SG), early individual counts of radicle emergence (RE), mean germination time (MGT), and vigor index (VI) for 16 commercially available seed lots of Siberian wildrye (Elymus sibiricus), an economically and ecological important grass species. The field emergence (FE) for seed lots was explored using a three-year field trial from 2021 to 2023. Meanwhile, pot experiments were carried out to determine the seedling emergence performance under different environmental conditions, including control, drought, and salinity stress. The correlation and regression analysis were done to investigate the links between laboratory vigor test with emergence performance under both field and pot conditions. The results showed that the SG of 16 seed lots were high, similar, and did not differ significantly between seed lots. However, the seedling emergence performance (emergence percentage, seedling dry weight, and simplified vigor index) differed significantly between seed lots under both field and pot conditions. The SG was not significantly correlated with seedling emergence performance under either the field or pot conditions. The counts of RE at selected timing, MGT, and VI showed a significant relationship with seed vigor as reflected by seedling emergence performance under diverse environmental conditions, and we found that RE at 108 h was highly predictive of seed vigor and seedling emergence. The RE at 108 h and VI were positively related to seed vigor, while MGT was negatively related. The seed lots with low vigor had lower RE at 108 h and VI but longer MGT. Overall, both RE at 108 h, MGT, and VI can be used to estimate seed vigor and predict seedling emergence under different environmental conditions, and we highly recommend RE at 108 h as a quick, precise, and convenient vigor test and early warning sign for seed storage of E. sibiricus. These results will enable seed researchers, plant breeders, farmers, and government program directors to target higher seed vigor more effectively for E. sibiricus and similar grass species.

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Effect of Dissolved Oxygen and Chemical Scarification on Andrographis paniculata Seed Germination in Macrobubble Conditions

Promwee

Islam

Khomphet

2023

International Journal of Agronomy

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Andrographis paniculata is used in Thai traditional medicine. This plant contains a bitter compound called andrographolide, which is highly effective in the prevention of many diseases. It is an effective treatment for infectious diseases and has a prophylactic effect owing to its powerful immunity-boosting benefits. Recently, it has been widely used to treat COVID-19. However, commercial planting of A. paniculata is performed by seeding, which leads to seed germination problems. The seed germination is relatively low and not efficient under normal conditions for various reasons, such as a combined dormancy of physical and innate nature, the diversity of the seeds in different lots, and the fact that the germination duration was not uniform in the same lot. An easily applied and inexpensive method for farmers to develop mass plantings to stimulate germination is by using macrobubble conditions by aerating seeds in sterile water in collaboration with chemical scarification, which is the idea of creating a hard seed coat that causes seed dormancy to break while root germination occurs at 25°C. Germination was completed after 16 days. The dissolved oxygen (DO) concentrations in this environment were 5, 6, 7, 8, and 9 mg·L−1. The oxygen intensity of 9 mg·L−1 showed the highest germination percentage (26.33%). It was found to be optimal for macrobubble conditions. Seedlings were treated with chemicals (PEG, NaCl, H2SO4, KCl, KNO3, NaHClO3, and GA3) after soaking in macrobubbles with optimum DO. The results showed that NaHClO3 conc. (30 min) showed a generation percentage reaching 92%, which could greatly promote up to 3.63 folds compared with the control in the macrobubble aeration system.

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Single count of radicle emergence and mean germination time estimate seed vigour of Chinese milk vetch (Astragalus sinicus)

Cited by 8 publications

References 37 publications

Hydrotime Model Parameters Estimate Seed Vigor and Predict Seedling Emergence Performance of Astragalus sinicus under Various Environmental Conditions

Hydrotime Model Parameters Estimate Seed Vigor and Predict Seedling Emergence Performance of Astragalus sinicus under Various Environmental Conditions

Siberian Wildrye (Elymus sibiricus) Seed Vigor Estimation for the Prediction of Emergence Performance under Diverse Environmental Conditions

Effect of Dissolved Oxygen and Chemical Scarification on Andrographis paniculata Seed Germination in Macrobubble Conditions

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