The advocacy of smart living results in a high demand for wearable and flexible sensors to monitor human motions. Among these, sensors based on strain−optics conversion are attractive due to their inherent electrical safety and electromagnetic immunity in comparison to strain−electricity conversion sensors. Particularly, hydrogel-based optical fiber sensors are biocompatible, flexible, and stretchable and thus are potentially applicable to health monitoring, human−machine intelligence, and soft robots. Nonetheless, hydrogelbased optical fibers still demonstrate challenges such as limited stretch ratios from chemical cross-linking networks and insufficient light transmittance from dehydration or nucleation of water. Herein, flexible and stretchable strain sensors based on glycerol-introducing nanocomposite hydrogel fibers (GN-Fibers) were achieved via dynamic stretching of a reactive pregel from monomer/nanoparticle hybrid precursors in a glycerol−water cosolvent. The resultant GN-Fibers evolved with anisotropic microstructures, displaying excellent tensile strength (9.76 MPa), high elastic modulus (32.63 MPa), low light propagation attenuation (0.26 dB cm −1 ), and broad strain range. Owing to the use of glycerol−water, such GN-Fibers also exhibited long-term moisture-retaining and antifreezing properties. In addition, GN-Fibers functioned well as sensors based on strain−optics conversion to monitor stretching and compressing behaviors. It is believed that such an optical fiber based strain sensor is a gateway to fabrication of next-generation wearable and flexible devices for health monitoring or artificial intelligence.