Single domain von Willebrand factor type C “cytokines” and the regulation of the stress/immune response in insects

Labropoulou, Vassiliki; Wang, Luoluo; Magkrioti, Christiana; Smagghe, Guy; Swevers, Luc

doi:10.1002/arch.22071

Cited by 5 publications

(1 citation statement)

References 69 publications

(152 reference statements)

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“…In these modules, expression in the cross-fostered larvae was consistently higher than control larvae. This stress effect was corroborated by significant functional enrichment in module 5 of regulation of metabolic and catabolic processes (GO:0019222, rank = 3, p = 2.9×10 −3 ; GO0031323, rank = 7, p = 5.3×10 −3 ; GO:0031329, rank = 1, p = 2.2×10 −3 ; GO:0009894, rank = 4, p = 3.8×10 −3 ; Figure S8F) and cytokine-like and interferon signaling and response (GO:0060338, rank = 5, p = 4.2×10 −3; GO:0060759, rank = 30, p = 2.2×10 −2 ), which are associated with immune system responses (Labropoulou et al, 2024; Rosche et al, 2021). Module 9 was enriched for midgut development (GO:0055123, GO:0007496, GO:0007494) and endothelial cell development and regeneration (Figure S8G).…”

Section: Resultsmentioning

confidence: 99%

Evolved and plastic gene expression in adaptation to a novel niche

Steward,

Ortega Giménez,

Choudhary

et al. 2024

Preprint

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Gene expression, resulting from complex regulatory interactions, plays an important role in adaptation and speciation. While gene expression historically has been studied in the context of reproductive isolation in speciation research, a role for evolved differences in gene expression in adaptation to novel niches is increasingly appreciated. How gene expression evolves and enables divergent ecological adaptation, and how changes in gene expression relate to genomic architecture and genetic divergence are pressing questions in understanding the processes of adaptation and ecological speciation. Further, how plasticity in gene expression can both contribute to and be affected by the process of ecological adaptation is a crucial component in understanding gene expression evolution. To address these questions, we investigate the role of evolved and plastic gene expression differences in adaptation leveraging an established host plant shift in the peacock fly Tephritis conura. Using a cross-fostering design where larvae feed on either natal or alternate host plants, we uncover extensive evolved differences in gene expression between the ecotypes, strikingly in genes associated with processing of host plant chemicals. We find limited evidence for plasticity, with some indications of higher plasticity in the ancestral ecotype where the expression of three gene coexpression modules is altered when larvae are cross-fostered to the derived host plant. Interestingly, we find an enrichment of differentially expressed genes within a large, ecotype-specific inversion in the T. conura genome. This finding adds to evidence that inversions are important for enabling diversification in the face of gene flow and underscores that effects on gene expression may be key to understanding the role of inversions.

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Section: Resultsmentioning

confidence: 99%

Evolved and plastic gene expression in adaptation to a novel niche

Steward,

Ortega Giménez,

Choudhary

et al. 2024

Preprint

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Single von Willebrand factor C-domain protein-2 confers immune defense against bacterial infections in the silkworm, Bombyx mori

Ma,

Wang,

Liu

et al. 2024

International Journal of Biological Macromolecules

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Aedes aegypti VLG-1challenges the assumed antiviral nature ofVagogenes

Couderc,

Crist,

Daron

et al. 2024

Preprint

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Arthropod-borne viruses (arboviruses) such as dengue virus (DENV) and Zika virus (ZIKV) pose a significant threat to global health. Novel approaches to control the spread of arboviruses focus on harnessing the antiviral immune system of their primary vector, theAedes aegyptimosquito. In arthropods, genes of theVagofamily are often presented as analogs of mammalian cytokines with potential antiviral functions, but the role ofVagogenes upon virus infection inAe. aegyptiis largely unknown. We conducted a phylogenetic analysis of theVagogene family in Diptera, which led us to focus on aVago-like gene that we namedVLG-1. Using CRISPR/Cas9-mediated gene editing, we generated aVLG-1mutant line ofAe. aegyptithat revealed a proviral effect of this gene upon DENV and ZIKV infection. In the absence ofVLG-1, virus dissemination throughout the mosquito’s body was impaired, albeit not altering virus transmission rates. A tissue-specific transcriptome analysis revealed that the loss ofVLG-1impacted numerous biological processes potentially linked to viral replication, such as the oxidative stress response. Our results challenge the conventional understanding ofVago-like genes as antiviral factors and underscores the need for further research to elucidate the molecular mechanisms underlying mosquito-arbovirus interactions.

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Single domain von Willebrand factor type C “cytokines” and the regulation of the stress/immune response in insects

Cited by 5 publications

References 69 publications

Evolved and plastic gene expression in adaptation to a novel niche

Evolved and plastic gene expression in adaptation to a novel niche

Single von Willebrand factor C-domain protein-2 confers immune defense against bacterial infections in the silkworm, Bombyx mori

Aedes aegypti VLG-1challenges the assumed antiviral nature ofVagogenes

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