Single equipment combines simultaneous application of mechanical ultrasound and photodynamic action for microbial control

Evaluation of photodynamic therapy on fibroblast viability and cytokine production

“…The concentration of curcumin PS associated with 72 J cm 2 of blue Led was biocompatible agreement with a previous in vivo study [28]. The present study employed a blue Led 480 nm, because curcumin PS has an absorption peak ranging between 300 and 500 nm, according with Paschoal et al [6].…”

“…The differences can be due to the photosensitizer, treatment protocol, or cell types. In the present study, it was used the previously described drug and light parameters [6]. After PDT, molecular biological mechanisms lead to biostimulation (increased proliferation rate), repair of the damage leading to rescue of the cells, autophagy, apoptosis and necrosis.…”

“…The PDT was performed with curcumin at 500 mg/L [1,7bis-(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione] (PDTPharma, Cravinhos, SP, Brazil), in the period of 60 s of preirradiation as recommended by Paschoal et al, 2013 [6]. Then the PS was activated with blue Led for 480 nm, 72 J cm 2 , (Physics Institute of São Carlos, University of São Paulo, São Carlos, SP, Brazil) for 300 s [6]. During the pre-irradiation of PS and also during the activation of the LED, the lighting of the laminar flow chamber was turned off and the ambient light in the laboratory was reduced to avoid PS degradation.…”

“…Recently new methods as photodynamic therapy (PDT) are used on treatments to promote disinfection in periodontitis, dental caries, endodontic diseases, among other dental specialties [5][6][7]. Photodynamic therapy uses specific wavelength light Laser (light amplification by stimulated emission of radiation) or Led http://dx.doi.org/10.1016/j.pdpdt.2016.01.007 1572-1000/© 2016 Elsevier B.V. All rights reserved.…”

“…Several kinds of PS may be associated with Laser or Led: hematoporphyrin derivatives, porphyrin, phenothiazine (toluidine blue and methylene blue), chlorophyll, fluorones (rhodamine B), cyanine phytotherapic agents, phthalocyanines and curcumin [5,11]. Curcumin, a compound from Curcuma longa L., the drug has a variety of applications, including treatment of liver diseases, wounds, inflamed joints and microbial effects [6,12,13].…”