Single-event tandem ICP-mass spectrometry for the quantification of chemotherapeutic drug-derived Pt and endogenous elements in individual human cells

Liu, Tong; Bolea-Fernández, Eduardo; Mangodt, Christophe; Wever, Olivier De; Vanhaecke, Frank

doi:10.1016/j.aca.2021.338797

Cited by 21 publications

(14 citation statements)

References 52 publications

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“…73 Furthermore, ICP-QMS/QMS has been covered by principle studies and reviews on the ICP-MS reaction/collision cell technology, 72,74,75 gas chromatography (GC-) ICP-MS, 76 and high performance liquid chromatography (HPLC-) ICP-MS for quantitative profiling analysis of metalloids in biological samples, 77 respectively. ion-gas molecule reaction, 73 isotope, 3,4,22-24,26-28,43-47,62-66,68, single particle, 5,6,15,31,40,[54][55][56][57] and chemical speciation, 7,8,[10][11][12]16,76,77 respectively.…”

Section: References Investigatedmentioning

confidence: 99%

“…Due to spectral interferences, the determination of lower concentrations (ng/mL order or lower) of P, S, Si, Cl, Br, and I is often challenging for traditional ICP-MS. Measurement by ICP-QMS/QMS at massshift mode using O2 as the cell gas permitted a better performance for analysis of these element and has been reported for P, 15,19,34,41,45,[49][50][51][52] S, 15,19,34,40,41,45,[44][45][46][47][48][49][50][51][52] Si, 19,34,45,50,52 Cl, 34,45,52 Br, 34,41 and I, 34,41 respectively.…”

Section: Reaction Gases Used In the Measurement By Icp-qms/qmsmentioning

confidence: 99%

“…24 Single particle analysis. Single particle (or single cell) analysis based on ICP-QMS/QMS was reported for Ti, 5 (Nd, Eu, P), 6 (P, S, Fe, Cu, Zn, Pt), 15 C, 31 Fe, 40,[54][55][56][57] (Au, Ag, Pt), 56 and (Cr, Al, Ni, Cu, Au, Pt) 57 , respectively. Complementary software package for single particle analysis can facilitate the data processing, which can also be achieved by using spreadsheet software such as Microsoft Excel.…”

Section: Reaction Gases Used In the Measurement By Icp-qms/qmsmentioning

confidence: 99%

“…5 Size detection limit of 0.6 μm was achieved for polystyrenebased microplastics by measuring 12 C + at on-mass mode with H2 as the reaction cell gas. 15 Measurement at on-mass mode by using H2 as the cell gas was effective for separating the spectral interferences in Fe3O4 nanoparticle analysis. 54 A slight broadening of peak width was observed with double the signal intensity in comparison to the measurement by HR-ICP-MS at pseudo-medium resolution mode (only the width of the entrance slit but not that of the exit slit being decreased).…”

Section: Reaction Gases Used In the Measurement By Icp-qms/qmsmentioning

confidence: 99%

See 3 more Smart Citations

Trends and Advances in Inductively Coupled Plasma Tandem Quadruple Mass Spectrometry (ICP-QMS/QMS) With Reaction Cell

Zhu¹

2021

At.Spectrosc.

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Trends and advances in the development and application of inductively coupled plasma tandem quadrupole mass spectrometry (ICP-QMS/QMS) with a reaction cell is reviewed mainly based on publications from January 2018 to July 2021. ICP-QMS/QMS has been applied in various research fields covering the sciences of biology, energy, environmental, food/medical, geology, materials, and radionuclide. The objectives of analysis cover the determination of elemental concentration, ion-gas reaction, isotope analysis, single particle analysis, and chemical speciation analysis. Measurement of most elements in the periodic table are reported except for H, N, O, F, rare gas, and some of the radionuclides. In addition to the default reaction/collision gases (i.e., He, H2, O2, and NH3), N2O, CO2, CH4, CH3F, C2H4, and C2H6 have been used as reaction gases to improve the capability of separating spectral interferences or to study the ion-molecule reactions. Typical applications of ICP-QMS/QMS analysis in the major research fields are also discussed.

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Section: References Investigatedmentioning

confidence: 99%

Section: Reaction Gases Used In the Measurement By Icp-qms/qmsmentioning

confidence: 99%

Section: Reaction Gases Used In the Measurement By Icp-qms/qmsmentioning

confidence: 99%

Section: Reaction Gases Used In the Measurement By Icp-qms/qmsmentioning

confidence: 99%

See 2 more Smart Citations

Trends and Advances in Inductively Coupled Plasma Tandem Quadruple Mass Spectrometry (ICP-QMS/QMS) With Reaction Cell

Zhu¹

2021

At.Spectrosc.

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“…Single-cell analysis based on inductively coupled plasma–mass spectrometry (ICP-MS) allows investigating heterogeneous cell populations with regard to cell numbers, cell types, and functionality. 1 , 2 Over the last decade, elemental single-cell analysis of cells in suspension 3 − 9 has evolved to the established strategy of mass cytometry, successfully applied in clinical routines. More recently, the potential of imaging strategies by laser ablation (LA)-ICPMS was recognized and has triggered a wave of exciting studies.…”

Section: Introductionmentioning

confidence: 99%

Cisplatin Uptake in Macrophage Subtypes at the Single-Cell Level by LA-ICP-TOFMS Imaging

et al. 2021

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A high-throughput laser ablation–inductively coupled plasma–time-of-flight mass spectrometry (LA-ICP-TOFMS) workflow was implemented for quantitative single-cell analysis following cytospin preparation of cells. For the first time, in vitro studies on cisplatin exposure addressed human monocytes and monocyte-derived macrophages (undifferentiated THP-1 monocytic cells, differentiated M0 macrophages, as well as further polarized M1 and M2 phenotypes) at the single-cell level. The models are of particular interest as macrophages comprise the biggest part of immune cells present in the tumor microenvironment and play an important role in modulating tumor growth and progression. The introduced bioimaging workflow proved to be universally applicable to adherent and suspension cell cultures and fit-for-purpose for the quantitative analysis of several hundreds of cells within minutes. Both, cross-validation of the method with single-cell analysis in suspension for THP-1 cells and with LA-ICP-TOFMS analysis of adherent M0 cells grown on chambered glass coverslips, revealed agreeing platinum concentrations at the single-cell level. A high incorporation of cisplatin was observed in M2 macrophages compared to the M0 and M1 macrophage subtypes and the monocyte model, THP-1. The combination with bright-field images and monitoring of highly abundant endogenous elements such as phosphorus and sodium at a high spatial resolution allowed assessing cell size and important morphological cell parameters and thus straightforward control over several cell conditions. This way, apoptotic cells and cell debris as well as doublets or cell clusters could be easily excluded prior to data evaluation without additional staining.

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Quantifying Intracellular Platinum Accumulation Using Inductively Coupled Mass Spectrometry

Krishnaraj,

Nair

2024

Current Protocols

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The platinum‐based anticancer drug cisplatin and its analog carboplatin are the most used chemotherapeutic agents worldwide. It is estimated that approximately half of all cancer patients are treated with platinum drugs at some point during the therapy regimen. Cisplatin covalently binds to purine nucleobases to form DNA adducts. Cisplatin therapy is faced with two key challenges. First, despite the initial response, many patients develop cisplatin resistance. Reduced cellular accumulation of cisplatin is one common cause of therapy resistance. Second, cisplatin treatment causes general cytotoxicity, leading to severe side effects. Monitoring the subcellular concentration of platinum chemotherapeutics will help yield clinical efficacy with the minimum possible dose. Inductively coupled plasma‐mass spectrometry (ICP‐MS) is an analytical technique to quantify the elemental composition of various types of liquified bulk samples with high sensitivity. This article describes quantifying cisplatin accumulation in chromatin and total cell lysate using ICP‐MS. The method involves treating cells with cisplatin, isolating RNA‐free DNA, digesting samples, ICP‐MS instrumentation, and data analysis. Although we describe these steps in one cancer cell line, the protocol can be adapted to any cell line or tissue. The protocol should be a valuable resource for investigators interested in accurate measurement of subcellular concentration of platinum and other metallo‐drugs. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Cell culture conditions for A2780 cells and cisplatin treatmentBasic Protocol 2: Isolating cellular fractions and sample quantitationBasic Protocol 3: Sample digestion, ICP‐MS data collection, and analysis

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Single-event tandem ICP-mass spectrometry for the quantification of chemotherapeutic drug-derived Pt and endogenous elements in individual human cells

Cited by 21 publications

References 52 publications

Trends and Advances in Inductively Coupled Plasma Tandem Quadruple Mass Spectrometry (ICP-QMS/QMS) With Reaction Cell

Trends and Advances in Inductively Coupled Plasma Tandem Quadruple Mass Spectrometry (ICP-QMS/QMS) With Reaction Cell

Cisplatin Uptake in Macrophage Subtypes at the Single-Cell Level by LA-ICP-TOFMS Imaging

Quantifying Intracellular Platinum Accumulation Using Inductively Coupled Mass Spectrometry

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