Single-exposure quantitative phase imaging in color-coded LED microscopy

Lee, Wonchan; Jung, Daeseong; Ryu, S.; Joo, Chulmin

doi:10.1364/oe.25.008398

Cited by 55 publications

(24 citation statements)

References 41 publications

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“…20 and Lee et al . 24 also demonstrated single-exposure quantitative phase imaging via color-multiplexed illumination, albeit with different color-encoding strategies and computation algorithms. In our mobile cLEDscope, the LED illumination in Fig.…”

Section: Resultsmentioning

confidence: 99%

Smartphone-based multi-contrast microscope using color-multiplexed illumination

Jung

Choi

Kim

et al. 2017

Sci Rep

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We present a portable multi-contrast microscope capable of producing bright-field, dark-field, and differential phase contrast images of thin biological specimens on a smartphone platform. The microscopy method is based on an imaging scheme termed “color-coded light-emitting-diode (LED) microscopy (cLEDscope),” in which a specimen is illuminated with a color-coded LED array and light transmitted through the specimen is recorded by a color image sensor. Decomposition of the image into red, green, and blue colors and subsequent computation enable multi-contrast imaging in a single shot. In order to transform a smartphone into a multi-contrast imaging device, we developed an add-on module composed of a patterned color micro-LED array, specimen stage, and miniature objective. Simple installation of this module onto a smartphone enables multi-contrast imaging of transparent specimens. In addition, an Android-based app was implemented to acquire an image, perform the associated computation, and display the multi-contrast images in real time. Herein, the details of our smartphone module and experimental demonstrations with various biological specimens are presented.

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Section: Resultsmentioning

confidence: 99%

Smartphone-based multi-contrast microscope using color-multiplexed illumination

Jung

Choi

Kim

et al. 2017

Sci Rep

Self Cite

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“…To mitigate the problem of color leakage or spectral cross talk, we use the color-leakage correction method. 38 In the method, the signal measured in a color channel is expressed as the sum of the light of the desired color and the lights of other colors, which can be written as E Q -T A R G E T ; t e m p : i n t r a l i n k -; e 0 0 1 ; 3 2 6 ; 3 9 5…”

Section: Multiwavelength Separationmentioning

confidence: 99%

Fourier ptychographic microscopy using wavelength multiplexing

Bian

Suo

et al. 2017

J. Biomed. Opt

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Fourier ptychographic microscopy (FPM) is a recently developed technique stitching low-resolution images in Fourier domain to realize wide-field high-resolution imaging. However, the time-consuming process of image acquisition greatly narrows its applications in dynamic imaging. We report a wavelength multiplexing strategy to speed up the acquisition process of FPM several folds. A proof-of-concept system is built to verify its feasibility. Distinguished from many current multiplexing methods in Fourier domain, we explore the potential of high-speed FPM in spectral domain. Compatible with most existing FPM methods, our strategy provides an approach to high-speed gigapixel microscopy. Several experimental results are also presented to validate the strategy.

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“…However, artifacts of phase reconstruction still cannot be completely avoided, since DPC's PTF is not circularly symmetric with only 2-axis measurements. Efforts have been made toward developing high speed, or even single-shot QPI mechanisms, such as color-coded DPC based on wavelength multiplexing [30,32,33]. Other efforts have been made to achieve isotropic DPC by either using more time-consuming intensity measurements along different illumination angles (12-axis) [20] or replacing the conventional half-circle illumination aperture with 3-axis radially asymmetric patterns [34] or 2-axis gradient amplitude patterns [35].…”

Section: Introductionmentioning

confidence: 99%

Optimal illumination scheme for isotropic quantitative differential phase contrast microscopy

et al. 2019

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Differential phase contrast microscopy (DPC) provides high-resolution quantitative phase distribution of thin transparent samples under multi-axis asymmetric illuminations. Typically, illumination in DPC microscopic systems is designed with 2-axis half-circle amplitude patterns, which, however, result in a non-isotropic phase contrast transfer function (PTF). Efforts have been made to achieve isotropic DPC by replacing the conventional half-circle illumination aperture with radially asymmetric patterns with 3-axis illumination or gradient amplitude patterns with 2-axis illumination. Nevertheless, these illumination apertures were empirically designed based on empirical criteria related to the shape of the PTF, leaving the underlying theoretical mechanisms unexplored. Furthermore, the frequency responses of the PTFs under these engineered illuminations have not been fully optimized, leading to suboptimal phase contrast and signal-to-noise ratio (SNR) for phase reconstruction. In this Letter, we provide a rigorous theoretical analysis about the necessary and sufficient conditions for DPC to achieve perfectly isotropic PTF. In addition, we derive the optimal illumination scheme to maximize the frequency response for both low and high frequencies (from 0 to 2N A ob j ), and meanwhile achieve perfectly isotropic PTF with only 2-axis intensity measurements. We present the derivation, implementation, simulation and experimental results demonstrating the superiority of our method over state-of-the-arts in both phase reconstruction accuracy and noise-robustness.

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Single-exposure quantitative phase imaging in color-coded LED microscopy

Cited by 55 publications

References 41 publications

Smartphone-based multi-contrast microscope using color-multiplexed illumination

Smartphone-based multi-contrast microscope using color-multiplexed illumination

Fourier ptychographic microscopy using wavelength multiplexing

Optimal illumination scheme for isotropic quantitative differential phase contrast microscopy

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