Single Fluorescent Probe for Dual-Imaging Viscosity and H₂O₂ in Mitochondria with Different Fluorescence Signals in Living Cells

Abstract

Mitochondria, as essential and interesting organelles within the eukaryotic cells, play key roles in a variety of pathologies, and its abnormalities are closely associated with Alzheimer's disease (AD) and other diseases. Studies have shown that the abnormal of viscosity and concentration of hydrogen peroxide in mitochondria were all associated with AD. Accordingly, the detection of viscosity and hydrogen peroxide in mitochondria has attracted great attention. However, it remains a great challenge to explore a single probe, which can dual-detect the viscosity and HO in mitochondria. Herein, in two ways to prevent the twisted internal charge transfer (TICT) process, we designed and sythesized the first dual-detection fluorescent probe Mito-VH that can visualize viscosity and HO in mitochondria with different fluorescence signals in living cells.

Fluorescent Molecular Rotors for Viscosity Sensors

“…Moreover,t he Lin group reported a dual sensing molecular probe with am itochondria-targeting moiety (pyridinium cation) that could simultaneously visualize viscosity and H 2 O 2 in the mitochondriao fR AW 264.7 cells with two-channel (blue/green)F Ls ignals. [80] Thioflavin-based FMRs (see Figure 8) has widely been used as af luorescent probe for detectiona nd structural studies of amyloid fibrils that are associated with neurodegeneratived iseases such as Alzheimer's and Parkinson's disease. [59,60,81] With ah igh binding affinity,T hioflavin-based FMRs incorporate into amyloid fibrils in the monomer form at its low concentration not exceeding 10 mm [82] and increases severalt housand times its fluorescenceq uantum yield due to the restricted internal rotationbyt he selectivebinding.…”

Research progress in the development of organic small molecule fluorescent probes for detecting H2O2

“…Hydrolysis of benzoborate ester groups was also used, Weiying Lin's research group 55 designed probe 17 (Fig. 12).…”

A simple sensor based on 1,8-naphthalimide with large Stokes shift for detection of hypochlorous acid in living cells

“…[15][16][17][18][19][20] Notably, uorescent methods have advantages of high sensitivity, good selectivity, strong specicity and fast response time, which is widely used in biological imaging. [21][22][23][24][25] In addition, in order to avoid serious selfabsorption of uorescence, probe molecules will be designed with large stokes shi, and when excited at the maximum absorption peak, the interference of excited light on uorescence signal will be greatly reduced.…”