Single Gene Mutations in Tomato Plants Regenerated from Tissue Culture

Abstract

Plants were regenerated from cultured leaf explants of an inbred variety of Lycopersicon esculentum. Seeds were collected from the regenerated plants and sown in the greenhouse. The resultant plants were then evaluated in the field. Several monogenic mutations segregated in the progeny of regenerated plants. The recovery of single gene mutations is evidence that plant tissue culture can be mutagenic. Complementation tests revealed that one mutation was located on the long arm of chromosome 10.

Variation in chromosome number in the seedling progeny of a somaclone of Paspalum dilatatum

“…There have been reports of somaclones of wheat and tomato with some variation in chromosome number, but their seed progeny had a normal chromosome complement [14,15]. In Panicum maximum, an apomictic plant, no variation in either phenotype or chromosome number was found in either the primary somaclones or their seedling progeny [16].…”

Plant Regeneration, Somaclonal Variation and Resistance to Late-blight in Tomato

“…There is frequent recovery of mutant in the R 1 generation (Shepard et al, 1980;Gavazzi et al, 1987). Evidence is lacking on their origin, however whatever the basis of this phenomenon, this is not new in the literature (Evans and Sharp, 1983;Larkin and Scowcroft, 1983). One of the major limitations to utilizing somaclonal variation in a breeding programme is the identification of desired mutant phenotypes.…”

“…The criterion of resistance used in this experiment for selection of a total of 17 seedlings was that most survived zoospore (10 4 /ml) inoculation of Ca 65 under environmental conditions which led to the death of the seedlings of LA 1623 control. The differences in disease response between control and resistant somaclones may be attributable primarily to mutations as they occur spontaneously and apparently at very high rates in cell cultures (Evans and Sharp, 1983). Hoswever, seedling survival may have been due to some seedlings accidentally receiving a smaller zoospore inoculum.…”

Obtaining Mutants from Cell Cultures

“…1983, EVANS and SHARP 1983. In addition, an increased number oi publications report on sequence alterations of DNA in nuclei, plastids and mitochondria (Cuti.is 1985, RODE et al 1987, BRFIMAN et al 1987, DEPAEPE et al 1982, U.MBFCK and GEGENBACH 1983 Lc)RZ and BROWN 1986), on amphfication of certain DNA regions (DL'RANTE et al 1983, CcLLis 1985, and on increased rates of mitotic recombination (SIBJ et al 1984).…”