Single laser source for multimodal coherent anti-Stokes Raman scattering microscopy

Pegoraro, Adrian F.; Slepkov, Aaron D.; Ridsdale, Andrew; Pezacki, John Paul; Stolow, Albert

doi:10.1364/ao.49.000f10

Cited by 19 publications

(13 citation statements)

References 53 publications

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“…Nonetheless, progress in laser technology may enable that these techniques will be implemented in a compact microscope comparable in size to the bright field microscopes nowadays widely used in hospitals. We believe that the implementation of fibre lasers [48][49][50][51] with their small footprint and robustness will bring CARS and nonlinear microscopy from specialized laser labs into hospital.…”

Section: Discussionmentioning

confidence: 99%

Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis

et al. 2011

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Contemporary brain tumor research focuses on two challenges: First, tumor typing and grading by analyzing excised tissue is of utmost importance for choosing a therapy. Second, for prognostication the tumor has to be removed as completely as possible. Nowadays, histopathology of excised tissue using haematoxylin-eosine staining is the gold standard for the definitive diagnosis of surgical pathology specimens. However, it is neither applicable in vivo, nor does it allow for precise tumor typing in those cases when only nonrepresentative specimens are procured. Infrared and Raman spectroscopy allow for very precise cancer analysis due to their molecular specificity, while nonlinear microscopy is a suitable tool for rapid imaging of large tissue sections. Here, unstained samples from the brain of a domestic pig have been investigated by a multimodal nonlinear imaging approach combining coherent anti-Stokes Raman scattering, second harmonic generation, and two photon excited fluorescence microscopy. Furthermore, a brain tumor specimen was additionally analyzed by linear Raman and Fourier transform infrared imaging for a detailed assessment of the tissue types that is required for classification and to validate the multimodal imaging approach. Hence label-free vibrational microspectroscopic imaging is a promising tool for fast and precise in vivo diagnostics of brain tumors.

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Section: Discussionmentioning

confidence: 99%

Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis

et al. 2011

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“…Such feedback is a common nuisance when using SCG-PCF modules [27]. However, because conventional wisdom dictates that for lownoise supercontinuum generation the SCG module must be pumped with short transformlimited pulses, any use of an isolator is invariably complicated by the added need for a pulse compressor [28]. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Spectrally-broad coherent anti-Stokes Raman scattering hyper-microscopy utilizing a Stokes supercontinuum pumped at 800 nm

Porquez

Cole

Tabarangao

et al. 2016

Biomed. Opt. Express

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Abstract:We demonstrate spectral-focusing based coherent anti-Stokes Raman scattering (SF-CARS) hyper-microscopy capable of probing vibrational frequencies from 630 cm 1 to 3250 cm 1 using a single Ti:Sapphire femtosecond laser operating at 800 nm, and a commercially-available supercontinuum-generating fibre module. A broad Stokes supercontinuum with significant spectral power at wavelengths between 800 nm and 940 nm is generated by power tuning the fibre module using atypically long and/or chirped ~200 fs pump pulses, allowing convenient access to lower vibrational frequencies in the fingerprint spectral region. This work significantly reduces the instrumental and technical requirements for multimodal CARS microscopy, while expanding the spectral capabilities of an established approach to SF-CARS.

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“…Whereas compact and mobile Raman systems have already been introduced, the laser sources for CARS are still large, complex, and expensive. The development of new laser-sources such as tunable, femtosecond fiber lasers 38,39,[48][49][50] are key elements to boost the applicability of nonlinear, Raman imaging and lead to further dissemination of the technique.…”

Section: Discussionmentioning

confidence: 99%

“…Generally various innovative laser concepts for coherent Raman microscopy have been realized recently mainly concentrating on compact, ideally turn-key, light sources based on all-fiber laser systems. 38,[48][49][50] …”

Section: Cars Microscopes For Biomedical Applicationsmentioning

confidence: 99%

Raman and coherent anti-Stokes Raman scattering microspectroscopy for biomedical applications

et al. 2012

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Abstract. A tutorial article is presented for the use of linear and nonlinear Raman microspectroscopies in biomedical diagnostics. Coherent anti-Stokes Raman scattering (CARS) is the most frequently applied nonlinear variant of Raman spectroscopy. The basic concepts of Raman and CARS are introduced first, and subsequent biomedical applications of Raman and CARS are described. Raman microspectroscopy is applied to both in-vivo and in-vitro tissue diagnostics, and the characterization and identification of individual mammalian cells. These applications benefit from the fact that Raman spectra provide specific information on the chemical composition and molecular structure in a label-free and nondestructive manner. Combining the chemical specificity of Raman spectroscopy with the spatial resolution of an optical microscope allows recording hyperspectral images with molecular contrast. We also elaborate on interfacing Raman spectroscopic tools with other technologies such as optical tweezing, microfluidics and fiber optic probes. Thereby, we aim at presenting a guide into one exciting branch of modern biophotonics research.

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Single laser source for multimodal coherent anti-Stokes Raman scattering microscopy

Cited by 19 publications

References 53 publications

Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis

Nonlinear microscopy, infrared, and Raman microspectroscopy for brain tumor analysis

Spectrally-broad coherent anti-Stokes Raman scattering hyper-microscopy utilizing a Stokes supercontinuum pumped at 800 nm

Raman and coherent anti-Stokes Raman scattering microspectroscopy for biomedical applications

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