Single-Layer Graphene Enhances the Osteogenic Differentiation of Human Mesenchymal Stem Cells <i>In Vitro</i> and <i>In Vivo</i>

Liu, Yunsong; Chen, Tong; Du, Feng; Gu, Ming; Zhang, Ping; Zhang, Xiao; Liu, Jianzhang; Lv, Longwei; Xiong, Chunyang; Zhou, Yongsheng

doi:10.1166/jbn.2016.2254

Cited by 53 publications

(35 citation statements)

References 39 publications

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“…It has been shown that graphene may enhance osteogenic differentiation of SCs [72,73]. Moreover, our recent data also suggest the beneficial impact of graphene oxide (GO) on proliferative capacity, viability and differentiation potential of umbilical cord tissue-derived MSCs, which confirms the possibility of future graphene employment in tissue repair [76].…”

Section: Synthetic Biomaterialssupporting

confidence: 62%

Biomaterials and Stem Cells: Promising Tools in Tissue Engineering and Biomedical Applications

Sekuła¹,

Zuba‐Surma²

2018

Biomaterials in Regenerative Medicine

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Biomaterial sciences and tissue engineering approaches are currently fundamental strategies for the development of regenerative medicine. Stem cells (SCs) are a unique cell type capable of self-renewal and reconstructing damaged tissues. At the present time, adult SCs isolated from postnatal tissues are widely used in clinical applications. Their characteristics such as a multipotent differentiation capacity and immunomodulatory activity make them a promising tool to use in patients. Modern material technologies allow for the development of innovative biomaterials that closely correspond to requirements of the current biomedical application. Biomaterials, such as ceramics and metals, are already used as implants to replace or improve the functionality of the damaged tissue or organ. However, the continuous development of modern technology opens new insights of polymeric and smart material applications. Moreover, biomaterials may enhance the SCs biological activity and their implementation by establishing a specific microenvironment mimicking natural cell niche. Thus, the synergistic advancement in the fields of biomaterial and medical sciences constitutes a challenge for the development of effective therapies in humans including combined applications of novel biomaterials and SCs populations.

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Section: Synthetic Biomaterialssupporting

confidence: 62%

Biomaterials and Stem Cells: Promising Tools in Tissue Engineering and Biomedical Applications

Sekuła¹,

Zuba‐Surma²

2018

Biomaterials in Regenerative Medicine

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“…Graphene coatings can provide an opportunity to overcome these limitations. Titanium and some of its industrially relevant alloys were shown to exhibit greatly improve cell adhesion and osteogenic differentiation of HMSCs and MC3T3‐E1 cells both in vitro and in vivo, when coated with graphene, rGO, and even GO ( Figure ) using polydopamine or PEI as adhesive layers …”

Section: Interactions Between Graphene Derivatives and Tissuesmentioning

confidence: 99%

Multivalent Interactions between 2D Nanomaterials and Biointerfaces

et al. 2018

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2D nanomaterials, particularly graphene, offer many fascinating physicochemical properties that have generated exciting visions of future biological applications. In order to capitalize on the potential of 2D nanomaterials in this field, a full understanding of their interactions with biointerfaces is crucial. The uptake pathways, toxicity, long-term fate of 2D nanomaterials in biological systems, and their interactions with the living systems are fundamental questions that must be understood. Here, the latest progress is summarized, with a focus on pathogen, mammalian cell, and tissue interactions. The cellular uptake pathways of graphene derivatives will be discussed, along with health risks, and interactions with membranes-including bacteria and viruses-and the role of chemical structure and modifications. Other novel 2D nanomaterials with potential biomedical applications, such as transition-metal dichalcogenides, transition-metal oxide, and black phosphorus will be discussed at the end of this review.

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“…The most dominant cell types used in the included articles were mesenchymal stem cells (MSCs), including human adipose-derived stem cells (hADSCs), [17][18][19][20][21][22] Goat ADSCs, 23 human dental pulp stem cells (hDPSCs), 24 human alveolar bone marrow stem cells (hABMSCs), 25 human periodontal ligament stem cells (hPDLSCs), 26,27 and bone MSCs (BMSCs), [28][29][30][31] such as human BMSCs 19, 32-41 (hBMSCs), murine BMSCs (mBMSCs), 4, 42-49 rabbit BMSCs (rBMSCs), 50 murine MSCs cell line C3H10T1/2 51,52 and Caprine BMSCs (cMBSCs). 53 The type of the MSCs in fifteen studies 19,32,36,40,[54][55][56][57][58][59][60][61][62][63][64] was not defined. Sixteen studies used preosteoblast cells, 14,[65][66][67][68][69][70][71][72][73][74]…”

Section: Cell Sourcesmentioning

confidence: 99%

“…Different types of graphene used in the included studies were pristine graphene, 19 36 GFs, 33 GNSs, 34 GQDs, 94 and nanosized graphene 18 (NGO).…”

Section: Graphene Typesmentioning

confidence: 99%

“…Cell adhesion and spread within the scaffold are usually the first steps following seeding of cells onto the biomaterials, and therefore, have a significant effect on modulating the forthcoming cell responses, including cell proliferation and differentiation. 47 In most reviewed studies, presence of graphene and its derivatives resulted in accelerated cell adhesion and spreading, 19,21,52 yet, there are few studies showing no difference between cell density on GO-coated substrates and noncoated substrate . 62,79 Besides, in a study by Kanayama et al, 70 GO and RGO films were found to show less DNA content compared to controls.…”

Section: Factors Affecting the Cellular Attachmentmentioning

confidence: 99%

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In vitro effect of graphene structures as an osteoinductive factor in bone tissue engineering: A systematic review

Mohammadrezaei

Golzar

Rad

et al. 2018

J Biomedical Materials Res

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Graphene and its derivatives have been well-known as influential factors in differentiating stem/progenitor cells toward the osteoblastic lineage. However, there have been many controversies in the literature regarding the parameters effect on bone regeneration, including graphene concentration, size, type, dimension, hydrophilicity, functionalization, and composition. This study attempts to produce a comprehensive review regarding the given parameters and their effects on stimulating cell behaviors such as proliferation, viability, attachment and osteogenic differentiation. In this study, a systematic search of MEDLINE database was conducted for in vitro studies on the use of graphene and its derivatives for bone tissue engineering from January 2000 to February 2018, organized according to the PRISMA statement. According to reviewed articles, different graphene derivative, including graphene, graphene oxide (GO) and reduced graphene oxide (RGO) with mass ratio ≤1.5 wt % for all and concentration up to 50 μg/mL for graphene and GO, and 60 μg/mL for RGO, are considered to be safe for most cell types. However, these concentrations highly depend on the types of cells. It was discovered that graphene with lateral size less than 5 µm, along with GO and RGO with lateral dimension less than 1 µm decrease cell viability. In addition, the three-dimensional structure of graphene can promote cell-cell interaction, migration and proliferation. When graphene and its derivatives are incorporated with metals, polymers, and minerals, they frequently show promoted mechanical properties and bioactivity. Last, graphene and its derivatives have been found to increase the surface roughness and porosity, which can highly enhance cell adhesion and differentiation. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2284-2343, 2018.

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Single-Layer Graphene Enhances the Osteogenic Differentiation of Human Mesenchymal Stem Cells In Vitro and In Vivo

Cited by 53 publications

References 39 publications

Biomaterials and Stem Cells: Promising Tools in Tissue Engineering and Biomedical Applications

Biomaterials and Stem Cells: Promising Tools in Tissue Engineering and Biomedical Applications

Multivalent Interactions between 2D Nanomaterials and Biointerfaces

In vitro effect of graphene structures as an osteoinductive factor in bone tissue engineering: A systematic review

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