We demonstrate mass-producible, tetherless microgrippers that can be remotely triggered by temperature and chemicals under biologically relevant conditions. The microgrippers use a selfcontained actuation response, obviating the need for external tethers in operation. The grippers can be actuated en masse, even while spatially separated. We used the microgrippers to perform diverse functions, such as picking up a bead on a substrate and the removal of cells from tissue embedded at the end of a capillary (an in vitro biopsy).actuator Í biochemical Í robotics Í thin films B iological function in nature is often achieved by autonomous organisms and cellular components triggered en masse by relatively benign cues, such as small temperature changes and biochemicals. These cues activate a particular response, even among large populations of spatially separated biological components. Chemically triggered activity is also often highly specific and selective in biological machinery. Additionally, mobility of autonomous biological entities, such as pathogens and cells, enables easy passage through narrow conduits and interstitial spaces.As a step toward the construction of autonomous microtools, we describe mass-producible, mobile, thermobiochemically actuated microgrippers. The microgrippers can be remotely actuated when exposed to temperatures ÏŸ40°C or selected chemicals. The temperature trigger is in the range experienced by the human body at the onset of a moderate-to-high fever, and the chemical triggers include biologically benign reagents, such as cell media. Using these microgrippers, we achieved a diverse set of functions, such as picking up beads off substrates and removing cells from tissue samples.Conventional microgrippers are usually tethered and actuated by mechanical or electrical signals (1-6). Recently developed actuation mechanisms using pneumatic (7), thermal (8), and electrochemical triggers (9, 10) have also used tethered operation. Because the functional response of currently available microgrippers is usually controlled through external wires or tubes, direct connections need to be made between the gripper and the control unit. These connections restrict device miniaturization and maneuverability. For example, a simple task such as the retrieval of an object from a tube is challenging at the millimeter and submillimeter scale, because tethered microgrippers must be threaded through the tube. Moreover, many of the schemes used to drive actuation in microscale tools use biologically incompatible cues, such as high temperature or nonaqueous media, which limit their utility. There are novel, untethered tools based on shape memory alloys that use low temperature heating, but they have limited mobility and must rely solely on thermal actuation (11,12). The ability of our gripper design to use biochemical actuation, in addition to thermal actuation, represents a paradigm shift in engineering and suggests a strategy for designing mobile microtools that function in a variety of environments with high specif...